Molecular Characterization of Rotaviruses with Distinct Group Antigens

Electrophoretic variation of avian rotavirus RNA in polyacrylamide gels

“…So far rotaviruses of electropherogroup 4 have been found only in chickens and those of electropherogroup 5 only in turkeys. RNA profiles of mammalian rotaviruses belonging to groups A, B and C (Pedley et al, 1983) are also shown in Fig. 1.…”

A human atypical rotavirus found in an Ecuador infant with diarrhea.

“…RNA species 7 to 9 of Wa strain, a typical group A virus, migrated as a tight triplet whereas, in new isolate, this triple was replaced by a doublet of species 8 and 9, and displaced species 7 migrated with species 5 and 6 to form a triplet. Such a genome profile corresponds to that of atypical rotavirus group C as reported by Pedley et al (1983). However, it still remains undetermined whether the present isolate has common group antigens of rotavirus group C. …”

“…The isolate was morphologically identical to known rotaviruses when observed by negative contrast electron microscopy (EM). The isolate, however, lacked a cross-reacting antigen of rotavirus, denoted as group A antigen by Pedley (Pedley et al 1983), as revealed by a enzyme-linked immunosorbent assay (ELISA) using either hyperimmune sera against Wa strain or a monoclonal antibody reacting with common group antigen A of rotavirus (Nakagomi et al 1984).…”

Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures

“…immunosorbent blocking assay, the existence of antigenically distinct rotaviruses lacking the common rotavirus group antigen has been recognised in humans (Dimitrov et al, 1983;Nicolas et al, 1983;Snodgrass et al, 1984), piglets (Saif et al, 1980;Bridger et al, 1982;Bohl et al, 1982;Pedley et al, 1983;Snodgrass et al, 1984;Bridger and Brown, 1985), calves and lambs (Snodgrass et al, 1984). Our isolate F-29 strain appears to lack the common rotavirus group antigen.…”