Electrophoretic variation of avian rotavirus RNA in polyacrylamide gels

Todd, D.; McNulty, M. S.

doi:10.1080/03079458608436274

Cited by 21 publications

(16 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…AvRV with the atypical RNA profile, characterized mainly by changes in the segregation characteristics of dsRNA segments 7, 8 and 9 were very frequent (21,26). Similarly, in this study AvRV with nine distinct electropherotypes was identified.…”

Section: Resultsmentioning

confidence: 71%

“…Even though there is no direct correlation between electropherotype and serotype, significant variations in eletrophoretic mobility of dsRNA segments can give rise to viral particles with antigenic variation in protein which induces neutralizing antibodies (21,26).…”

Section: Resultsmentioning

confidence: 99%

“…E-mail: alfieri@uel.br segments or electropherotype. This methodology, which is used in human and animal rotavirus diagnosis, is easy to use and allows identification of other segmented dsRNA virus such as picobirnavirus (1,2,3,10,26).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken

Tamehiro

Alfieri

Médici

2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

Segmented double-stranded RNA (dsRNA) viruses were identified by polyacrylamide gel electrophoresis (PAGE) technique in fecal samples from broiler chicken. A total of 378 fecal samples from 1-7 weeks old chickens were analyzed. dsRNA with migration profile characteristic of avian rotavirus (AvRV), reovirus (ARV) or picobirnavirus (PBV) was identified in 32 (8.5%), 7 (1.8%) and 13 (3.4%) samples, respectively. AvRV and ARV occurred more frequently in chickens up to 1 month old and were related with enteritis signs. Considering only fecal samples of chickens with diarrhea, the AvRV was detected in 37.8% (14/37) and the ARV in 13.5% (5/37) of analyzed samples. AvRV was identified in only 1.5% (4/274) and ARV was not detected in normal feces collected from assymptomatic chickens (controls). PBV dsRNA was detected in broiler chickens from two to seven weeks old, more frequently in feces with pasty consistency. The AvRV showed great electrophoretic profile variability in the dsRNA segments and nine different electropherotypes were identified. Variation in genome pattern was not observed in either ARV or PBV.

show abstract

Section: Resultsmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken

Tamehiro

Alfieri

Médici

2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…One was the obvious genomic difference between the pigeon and conventional group A mammalian rotaviruses. The electrophoretic migration profile of genomic RNA from our isolate most probably corresponds to the electrophero-group one described by Todd & McNulty (1986), although comparative co-electrophoresis experiments were not done. Another was the antigenic differences revealed by cross-neutralization tests between the pigeon virus and mammalian rotavirus.…”

Section: Discussionmentioning

confidence: 69%

Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures

Minamoto¹,

Oki²,

Tomita³

et al. 1988

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYAvian rotaviruses were isolated from feral pigeon faeces treated with trypsin using roller tube cultures of mammalian cells. Two pigeon strains, designated as strains PO-8 and PO-13, produced a marked cytopathic effect (CPE), small intracytoplasmic inclusion bodies and high titres of infectious particles in infected MA-104 and MDBK cell lines without cell adaptation and roller drum apparatus. The pigeon rotaviruses shared a common group specific antigen with the Lincoln strain of bovine rotavirus by indirect immunofluorescence, but differed from both the Lincoln strain and the Wa strain of human rotavirus in neutralization tests.

show abstract

“…This teschnique was also used to study avian rotaviruses from chickens (McNulty et al, 1981) and turkeys (Saif et al, 1985) and, according to the migration pattern on silver-stained PAGE, avian rotaviruses were classified into different eletropherogroups (Kang et al, 1986;Todd & McNulty, 1986) and eletropherotypes.…”

Section: Genome Electropherotyping By Polyacrylamide Gel Electrophoresismentioning

confidence: 99%

Enteric viruses in turkey flocks: a historic review

Alavarez

Ferreira

2014

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

In this review, diagnostic techniques and viral agents involved in enteric diseases affecting turkeys are described. Data from field observations and laboratory researches have been reported in turkey flocks for over 70 years, and several viruses have been identified. After a period of 30 years of inoculation experiments and neutralization studies, adequate visualization of the viruses was achieved using electronic microscopy. During the following years, several studies were then conducted to isolate and classify those viruses using cell-culture, embryo-propagation, serological tests, genome electropherotyping by polyacrylamide gel electrophoresis of double-stranded RNA viruses, and recently, nucleic acid studies. Thus, since the 1990s, the nucleicacid technology has focused on genomic surveys and on the detection of specific segments of the genome of each virus using the polymerasechain reaction, resulting in several prevalence studies and phylogenetic analyses of different isolates and proper classification of the viruses.

show abstract

Electrophoretic variation of avian rotavirus RNA in polyacrylamide gels

Cited by 21 publications

References 9 publications

Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken

Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken

Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures

Enteric viruses in turkey flocks: a historic review

Contact Info

Product

Resources

About