Molecular characterization of rotavirus Group A VP6 gene in Egyptian surface water, wastewater and diarrheal specimens

Kamel, Marwa A.; Barakat, Ahmed B.; El-Sayed, Aly F M; El‐Senousy, Waled M.; Elsayed, Omar El-Farouk Rabia

doi:10.21608/ejabf.2020.115070

Cited by 2 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our dataset indicated a high similarity to those of human RVA strains and particularly human Wa-like strains in the USA. These findings were in accordance with another studies in Egypt that reported the similarity between the VP6 and VP4 genes of RVA isolated from clinical and environmental samples and the Wa reference strain in the USA [ 12 , 40 ]. The low variations in VP6 sequences contained in the Egyptian G1P8 genotype may suggest that VP6 recombinant subunit vaccines would be useful candidates for rotavirus vaccination among Egyptian children.…”

Section: Discussionsupporting

confidence: 93%

“…The current study is one of a handful of research projects on rotavirus disease in children under the age of five that has been conducted in Egypt [ 12 , 21 , 38 , 39 , 40 ]. This study reports the distribution of rotavirus isolated from fecal samples in a repeated cross-sectional study of 230 Egyptian children, ranging in age from birth to five years, between 2018 and 2020 in two major university hospitals in Egypt.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, Propagation and Genotyping of Human Rotaviruses Circulating among Children with Gastroenteritis in Two Egyptian University Hospitals

El-Gayar

Saleh

Mohamed³

et al. 2022

Biology

View full text Add to dashboard Cite

The most prevalent cause of infectious neonatal diarrhea is Group A rotavirus (RVA). Unfortunately, there is a dearth of data on the incidence of rotavirus-associated infections among Egyptian children. The present study aimed to isolate, propagate, and genotype human rotaviruses circulating among Egyptian children with acute gastroenteritis admitted to two main university pediatric hospitals, Abo El-Reesh and El-Demerdash, over two consecutive winters, 2018–2020. Diarrheal samples (n = 230) were screened for Group A rotavirus RNA using RT-PCR assay. In positive samples (n = 34), multiplex semi-nested PCR was utilized to determine G and P genotypes. Thirty-four (14.8%) of the collected samples tested positive. The genotype distribution revealed that G1P[8] was the predominant rotavirus genotype throughout the current study. All rotavirus-positive fecal samples were passaged twice on human colorectal adenocarcinoma cell line (Caco-2) and rhesus monkey kidney epithelial cell line (MA104). Both cell lines could successfully isolate 14.7% (n = 5 out of 34) of the identified strains; however, Caco-2 cell line was shown to be more efficient than MA104 in promoting the propagation of human rotaviruses identified in Egyptian children’s feces.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Isolation, Propagation and Genotyping of Human Rotaviruses Circulating among Children with Gastroenteritis in Two Egyptian University Hospitals

El-Gayar

Saleh

Mohamed³

et al. 2022

Biology

View full text Add to dashboard Cite

show abstract

Neutralization and immunoperoxidase to estimate the immunogenicity of recombinant human rotavirus VP6 structural protein in vitro after insertion of the antibodies into cells using electroporation

Kamel,

Shokeer,

Hegazy

et al. 2024

Preprint

View full text Add to dashboard Cite

Rotaviruses (RVs) represent the principal causative agent of severe gastroenteritis leading to high mortality rates, especially in children < 5 years in both developed and developing countries. Although, the first-generation of live attenuated RV vaccines such as RotaTeq and Rotarix achieved partial success in reducing the number of RV deaths worldwide, several concerns, such as low efficacy especially in developing countries, safety, and cost imply a dire need to develop these vaccines. Also, sensitive methods to estimate the immunogenicity of the candidate recombinant subunit VP6 vaccines in vitro are of great need. In the present study, 1232 bp of the most frequent full length VP6 in clinical and environmental isolates in Egypt with 98% nucleotides identity and 98% amino acid identity in comparison to human RoV Wa reference strain was expressed in E.coli. The examination of the sensitivity of the antibodies produced in the male rabbits which were immunized intramuscularly with 20 µg of the purified VP6 proteins, indicated a sensitivity up to 1/24000 dilution of antibodies against the expressed protein using ELISA. Introduce antibodies into MA104 cell line was performed using electroporation to neutralize the human rotavirus Wa strain VP6 when exposed after viral uncoating. Higher sensitivity of neutralization in relation to immunoperoxidase was observed for the estimation of the antibodies which act intracellularly against high and low infectious units of human rotavirus Wa strain in vitro. Promising sensitivity of the produced antibodies against the infectious human RV Wa strain was observed.

show abstract

Molecular characterization of rotavirus Group A VP6 gene in Egyptian surface water, wastewater and diarrheal specimens

Abstract: Impact of rotavirus vaccine introduction and genotypic characteristics of rotavirus strains in children less than 5 years of age with gastroenteritis in Ethiopia: 2011-2016. Vaccine, 36: 7043-7047. American Public Health Association (APHA). (1998). Standard methods. 20 th Edition.

Cited by 2 publications

References 41 publications

Isolation, Propagation and Genotyping of Human Rotaviruses Circulating among Children with Gastroenteritis in Two Egyptian University Hospitals

Isolation, Propagation and Genotyping of Human Rotaviruses Circulating among Children with Gastroenteritis in Two Egyptian University Hospitals

Neutralization and immunoperoxidase to estimate the immunogenicity of recombinant human rotavirus VP6 structural protein in vitro after insertion of the antibodies into cells using electroporation

Contact Info

Product

Resources

About