Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering

Zhou, Wei; Bi, Jian; Janson, Jan‐Christer; Li, Yan; Huang, Yongdong; Zhang, Yan; Su, Zhiguo

doi:10.1016/j.jchromb.2006.03.064

Cited by 34 publications

(15 citation statements)

References 26 publications

(35 reference statements)

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“…DCs were prepared by treating the monocyte population with GM-CSF (500 U/mL) and recombinant human IL-4 for five days, and then treated with HBsAg, mannan, ammonium chloride, chloroquine, Dynasore (Sigma-Aldrich, St. Louis, MO, USA), HBsAg-blocking antibody, or neutralizing anti-MR antibody (clone 19.2, BD). The recombinant HBsAg and HSA were prepared from Chinese Hamster Ovary ( CHO ) cells as previously described [51]. The purity of both HBsAg and HSA reached 99%, as determined by high performance liquid chromatography.…”

Section: Methodsmentioning

confidence: 99%

Hepatitis B Virus Induces IL-23 Production in Antigen Presenting Cells and Causes Liver Damage via the IL-23/IL-17 Axis

et al. 2013

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IL-23 regulates myriad processes in the innate and adaptive immune systems, and is a critical mediator of the proinflammatory effects exerted by Th17 cells in many diseases. In this study, we investigated whether and how hepatitis B virus (HBV) causes liver damage directly through the IL-23 signaling pathway. In biopsied liver tissues from HBV-infected patients, expression of both IL-23 and IL-23R was remarkably elevated. In vivo observations also indicated that the main sources of IL-23 were myeloid dendritic cells (mDCs) and macrophages. Analysis of in vitro differentiated immature DCs and macrophages isolated from healthy donors revealed that the HBV surface antigen (HBsAg) efficiently induces IL-23 secretion in a mannose receptor (MR)-dependent manner. Culture with an endosomal acidification inhibitor and the dynamin inhibitor showed that, upon binding to the MR, the HBsAg is taken up by mDCs and macrophages through an endocytosis mechanism. In contrast, although the HBV core antigen (HBcAg) can also stimulate IL-23 secretion from mDCs, the process was MR- and endocytosis-independent. In addition, IL-23 was shown to be indispensible for HBsAg-stimulated differentiation of naïve CD4+ T cells into Th17 cells, which were determined to be the primary source of IL-17 in HBV-infected livers. The cognate receptor, IL-17R, was found to exist on the hepatic stellate cells and mDCs, both of which might represent the potential target cells of IL-17 in hepatitis B disease. These data provide novel insights into a yet unrecognized mechanism of HBV-induced hepatitis, by which increases in IL-23 expression, through an MR/endocytosis-dependent or -independent manner, produce liver damage through the IL-23/IL-17 axis.

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Section: Methodsmentioning

confidence: 99%

Hepatitis B Virus Induces IL-23 Production in Antigen Presenting Cells and Causes Liver Damage via the IL-23/IL-17 Axis

et al. 2013

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“…Both of the shapes of HBsAg normal assembly and large size aggregates are spherical monitored by transmission electron microscope (TEM) [21], so the shape of the assembly and aggregates in solution can be supposed to be sphere and the particle diameter can be calculated based on their spherical shape. Based on the assay of HPSEC-MALLS, the average molecular of these aggregates was about 4.06 Â 10 7 Da, 10 times higher than that of Han-HBsAg assembly (3.01 Â 10 6 Da) and the particle diameter of the aggregates was about 33.3 nm, almost two times bigger than that of HBsAg assembly of 18.1 nm [22].…”

Section: Aggregation Of Hbsag Assembly In the Process Of Ultrafiltrationmentioning

confidence: 92%

Characterization of the large size aggregation of Hepatitis B virus surface antigen (HBsAg) formed in ultrafiltration process

Zhou

et al. 2007

Process Biochemistry

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“…Purified HBsAg (∼3500 kDa, IEP of 4.5; obtained from GlaxoSmithKline) was dissolved in 150 mM NaCl and 10 mM phosphate buffer, pH 6.8. Dilutions of HBsAg were prepared using the same buffer.…”

Section: Methodsmentioning

confidence: 99%

Development of a High-Throughput Screening Platform to Study the Adsorption of Antigens onto Aluminum-Containing Adjuvants

Jully

Moniotte

Mathot

et al. 2015

Journal of Pharmaceutical Sciences

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Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering

Cited by 34 publications

References 26 publications

Hepatitis B Virus Induces IL-23 Production in Antigen Presenting Cells and Causes Liver Damage via the IL-23/IL-17 Axis

Hepatitis B Virus Induces IL-23 Production in Antigen Presenting Cells and Causes Liver Damage via the IL-23/IL-17 Axis

Characterization of the large size aggregation of Hepatitis B virus surface antigen (HBsAg) formed in ultrafiltration process

Development of a High-Throughput Screening Platform to Study the Adsorption of Antigens onto Aluminum-Containing Adjuvants

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