Characterization of the large size aggregation of Hepatitis B virus surface antigen (HBsAg) formed in ultrafiltration process

Li, Yan; Bi, Jian; Zhou, Wei; Huang, Yongdong; Sun, Lina; Zeng, An‐Ping; Ma, Guanghui; Su, Zhiguo

doi:10.1016/j.procbio.2006.08.016

Cited by 25 publications

(10 citation statements)

References 18 publications

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“…Note that the protruded surface features of the HBsAg particles are much better defined after KSCN-induced oxidative maturation with more interchain cross-linking via disulfide bonds (Wampler et al 1985). Size characterization and monitoring of aggregation during processing steps could be monitored by CD and HPSEC-MALLS (Li et al 2007).…”

Section: Modern Characterization Methodsmentioning

confidence: 99%

Recombinant Virus-like Particle Protein Vaccines

Sitrin¹,

Zhao

Potter

et al. 2014

Vaccine Analysis: Strategies, Principles, and Control

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Section: Modern Characterization Methodsmentioning

confidence: 99%

Recombinant Virus-like Particle Protein Vaccines

Sitrin¹,

Zhao

Potter

et al. 2014

Vaccine Analysis: Strategies, Principles, and Control

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“…Although various SLPPs have been successfully purified with chromatography and membrane‐based separation techniques, there are many reports on denaturation or significant loss of SLPPs during these two methods [15]. For example, in the purification of hepatitis B surface antigen (HBs VLPs), a single ion exchange chromatography (IEC) or ultrafiltration step would lead to 20–40% loss in the total antigen recovery [16, 17], accompanied with the VLPs aggregation and dissociation. These failures expose the fact that we still do not fully understand the denaturation mechanisms and how to stabilize SLPPs during purification.…”

Section: Challenges In Manufacturing Of Slppsmentioning

confidence: 99%

Characterization and stabilization in process development and product formulation for super large proteinaceous particles

Yang

et al. 2020

Engineering in Life Sciences

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Super large proteinaceous particles (SLPPs) such as virus, virus like particles, and extracellular vesicles have successful and promising applications in vaccination, gene therapy, and cancer treatment. The unstable nature, the complex particulate structure and composition are challenges for their manufacturing and applications. Rational design of the processing should be built on the basis of fully understanding the characteristics of these bio‐particles. This review highlights useful analytical techniques for characterization and stabilization of SLPPs in the process development and product formulations, including high performance size exclusion chromatography, multi‐angle laser light scattering, asymmetrical flow field‐flow fractionation, nanoparticle tracking analysis, CZE, differential scanning calorimetry, differential scanning fluorescence, isothermal titration calorimetry , and dual polarization interferometry. These advanced analytical techniques will be helpful in obtaining deep insight into the mechanism related to denaturation of SLPPs, and more importantly, in seeking solutions to preserve their biological functions against deactivation or denaturation. Combination of different physicochemical techniques, and correlation with in vitro or in vivo biological activity analyses, are considered to be the future trend of development in order to guarantee a high quality, safety, and efficacy of SLPPs.

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“…The group has successfully refolded some proteins, such as Hepatitis B virus surface antigen (HBsAg), Consensus interferon (C-IFN), etc. [74,75].…”

Section: Renaturation and Modification Of Proteinsmentioning

confidence: 99%

Bioreactors and Bioseparation

Zhang

Cao

Chu

et al. 2010

Biotechnology in China II

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Along with the rapid development of life science, great attention has been increasingly given to the biotechnological products of cell cultivation technology. In the course of industrialization, bioreactor and bioproduct separation techniques are the two essential technical platforms. In this chapter, the current situation and development prospects of bioreactor techniques in China are systematically discussed, starting with the elucidation of bioreactor processes and the principle of process optimization. Separation technology for biological products is also briefly introduced.At present, a series of bioreactors made by Chinese enterprises have been widely used for laboratory microbial cultivation, process optimization studies, and large-scale production. In the course of bioprocess optimization studies, the complicated bioprocesses in a bioreactor could be resolved into different reaction processes on three scales, namely genetic, cellular, and bioreactor scales. The structural varieties and nonlinear features of various scales of bioprocess systems was discussed through considering the mutual effects of different scale events, namely material flux, energy flux, and information flux, and the optimization approach for bioprocesses was proposed by taking the analysis of metabolic flux and multiscale consideration as a core strategy.In order to realize such an optimization approach, a bioreactor system based on association analysis of multiscale parameters was elaborated, and process optimization of many biological products were materialized, which resulted in great improvement in production efficiency. In designing and manufacturing large-scale bioreactors, the principle of scaling up a process incorporated with flow field study and physiological features in a bioreactor was suggested according to the criterion for the scale-up of cellular physiological and metabolic traits. The flow field features of a bioreactor were investigated through computational fluid dynamics (CFD), and the design of a bioreactor configuration was carried out depending on multiscale studies of parameters correlation in a bioprocess. With respect to the development trend of bioreactor studies in China, the significance of the development of microbioreactors for high throughput strain screening and large-scale bioreactors for animal cell cultivation were put forward. Finally, the importance of studies of systems biology for bioprocesses based on bioinformation processing was raised, and the necessity of establishing a bioprocess information database and local area network (LAN) were emphasized as well.Bioseparation engineering plays a key role in biotechnology production. At present, many difficulties need to be resolved in the area. Scientists of China have made considerable progress in bioseparation engineering. This progress includes chromatography media, recycling aqueous two-phase systems (ATPS), affinity precipitation, molecular imprinting, renaturation and modification of proteins, protein fractionation using ultrafiltration (UF), i...

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Characterization of the large size aggregation of Hepatitis B virus surface antigen (HBsAg) formed in ultrafiltration process

Cited by 25 publications

References 18 publications

Recombinant Virus-like Particle Protein Vaccines

Recombinant Virus-like Particle Protein Vaccines

Characterization and stabilization in process development and product formulation for super large proteinaceous particles

Bioreactors and Bioseparation

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