Molecular Characterization of Mungbean (<i>Vigna radiata</i> L.) Starch Branching Enzyme I

Chang, Jia‐Wei; Li, Sing Chung; Shih, Yun Chi; Wang, Reuben; Chung, Pei Shan; Ko, Yuan Tih

doi:10.1021/jf102129f

Cited by 6 publications

(4 citation statements)

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“…Three isoforms of SBE (SBEI, SBEIIa, and SBEIIb) were detected in maize, rice, wheat and barley [25, 39–42], while in Vigna radiate [43,44], Ipomoea batatas [45]and Phaseolus vulgaris [46], SBE had been classified into SBE I and SBE II isoforms. In Arabidopsis , SBE I did not expressed and its function was unknown, while SBE II had been classified into SBE 2.1 and SBE 2.2 [47].…”

Section: Discussionmentioning

confidence: 99%

Identification and expression analysis of starch branching enzymes involved in starch synthesis during the development of chestnut (Castanea mollissima Blume) cotyledons

Chen

Wang

et al. 2017

PLoS ONE

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Chinese chestnut (Castanea mollissima Blume) is native to China and distributes widely in arid and semi-arid mountain area with barren soil. As a perennial crop, chestnut is an alternative food source and acts as an important commercial nut tree in China. Starch is the major metabolite in nuts, accounting for 46 ~ 64% of the chestnut dry weight. The accumulation of total starch and amylopectin showed a similar increasing trend during the development of nut. Amylopectin contributed up to 76% of the total starch content at 80 days after pollination (DAP). The increase of total starch mainly results from amylopectin synthesis. Among genes associated with starch biosynthesis, CmSBEs (starch branching enzyme) showed significant increase during nut development. Two starch branching enzyme isoforms, CmSBE I and CmSBE II, were identified from chestnut cotyledon using zymogram analysis. CmSBE I and CmSBE II showed similar patterns of expression during nut development. The accumulations of CmSBE transcripts and proteins in developing cotyledons were characterized. The expressions of two CmSBE genes increased from 64 DAP and reached the highest levels at 77 DAP, and SBE activity reached its peak at 74 DAP. These results suggested that the CmSBE enzymes mainly contributed to amylopectin synthesis and influenced the amylopectin content in the developing cotyledon, which would be beneficial to chestnut germplasm selection and breeding.

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Section: Discussionmentioning

confidence: 99%

Identification and expression analysis of starch branching enzymes involved in starch synthesis during the development of chestnut (Castanea mollissima Blume) cotyledons

Chen

Wang

et al. 2017

PLoS ONE

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“…The branching enzymes be1 and be2 play different roles in the structure of amylopectin in storage organs [31,46]. The be1 knockout mutants observed display no particular phenotype, except in maize [4,16] where be1 appears to be required for starch mobilization during seed germination, and in Chlamydomonas where be1 mutants are defective for starch catabolism [7,47,48].…”

Section: Discussionmentioning

confidence: 99%

Molecular evolution accompanying functional divergence of duplicated genes along the plant starch biosynthesis pathway

et al. 2014

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BackgroundStarch is the main source of carbon storage in the Archaeplastida. The starch biosynthesis pathway (sbp) emerged from cytosolic glycogen metabolism shortly after plastid endosymbiosis and was redirected to the plastid stroma during the green lineage divergence. The SBP is a complex network of genes, most of which are members of large multigene families. While some gene duplications occurred in the Archaeplastida ancestor, most were generated during the sbp redirection process, and the remaining few paralogs were generated through compartmentalization or tissue specialization during the evolution of the land plants. In the present study, we tested models of duplicated gene evolution in order to understand the evolutionary forces that have led to the development of SBP in angiosperms. We combined phylogenetic analyses and tests on the rates of evolution along branches emerging from major duplication events in six gene families encoding sbp enzymes.ResultsWe found evidence of positive selection along branches following cytosolic or plastidial specialization in two starch phosphorylases and identified numerous residues that exhibited changes in volume, polarity or charge. Starch synthases, branching and debranching enzymes functional specializations were also accompanied by accelerated evolution. However, none of the sites targeted by selection corresponded to known functional domains, catalytic or regulatory. Interestingly, among the 13 duplications tested, 7 exhibited evidence of positive selection in both branches emerging from the duplication, 2 in only one branch, and 4 in none of the branches.ConclusionsThe majority of duplications were followed by accelerated evolution targeting specific residues along both branches. This pattern was consistent with the optimization of the two sub-functions originally fulfilled by the ancestral gene before duplication. Our results thereby provide strong support to the so-called “Escape from Adaptive Conflict” (EAC) model. Because none of the residues targeted by selection occurred in characterized functional domains, we propose that enzyme specialization has occurred through subtle changes in affinity, activity or interaction with other enzymes in complex formation, while the basic function defined by the catalytic domain has been maintained.

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“…In quantitative real time-PCR (qRT-PCR) experiment, gene-specific primers were employed to amplify a set of mungbean phytohormones and housekeeping genes, and their details are presented in Table 4 . For indigenous primers, VrTUB and β-Actin primers were chosen in accordance with Chang et al. (2010) and Sairam et al.…”

Section: Methodsmentioning

confidence: 99%

UHPLC-MS/MS and QRT-PCR profiling of PGP agents and Rhizobium spp. of induced phytohormones for growth promotion in mungbean (var. Co4)

Mogal

Solanki²,

Kansara³

et al. 2022

Heliyon

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Molecular Characterization of Mungbean (Vigna radiata L.) Starch Branching Enzyme I

Cited by 6 publications

References 30 publications

Identification and expression analysis of starch branching enzymes involved in starch synthesis during the development of chestnut (Castanea mollissima Blume) cotyledons

Identification and expression analysis of starch branching enzymes involved in starch synthesis during the development of chestnut (Castanea mollissima Blume) cotyledons

Molecular evolution accompanying functional divergence of duplicated genes along the plant starch biosynthesis pathway

UHPLC-MS/MS and QRT-PCR profiling of PGP agents and Rhizobium spp. of induced phytohormones for growth promotion in mungbean (var. Co4)

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