Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients

Nairoukh, Yacoub R.; Mahafzah, Azmi; Irshaid, Amal; Shehabi, Asem A.

doi:10.2174/1874285801812010001

Cited by 19 publications

(15 citation statements)

References 28 publications

(36 reference statements)

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“…Misappropriation of antibiotic is liable for the higher incidence of antibiotic resistance among bacteria(Neupane et al, 2016). In this study, 65% E. coli isolates were multidrug resistant which are in agreement with a number of studies, 95.52%(Yadav et al, 2015), 73.68%(Thakur et al, 2013), 57.3%(Nairoukh et al, 2018) and 40.7% isolates in Tehran(Ghadiri et al, 2014). In this study, E. coli isolates exhibited variable rates of susceptibility and resistance to 10 different types of antibiotics.…”

supporting

confidence: 90%

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Fazlul¹,

Najnin²,

Yasmin³

et al. 2019

IJPR

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The multidrug resistant Escherichia coli strains causes multiple clinical infections and has become a rising problem globally. The metallo-β-lactamases encoding genes are very severe in gram-negative bacteria especially E. coli. This study was aimed to evaluate the prevalence of MBLs among the clinical isolates of E. coli. A total of 65 E. coli isolates were collected from various clinical samples of Malaysian patients with bacterial infections. The conventional microbiological test was performed for isolation and identification of E. coli producing MBLs strains in this vicinity. Multidrug Resistance (MDR) of E. coli isolates were assessed using disk diffusion test. Phenotypic methods, as well as genotypic-PCR methods, were performed to detect the presence of metallo-β-lactamase resistance genes (blaIMP, blaVIM) in imipenem resistant strains. Out of 65 E. coli isolates, 42 isolates (57.3%) were MDR. The isolates from urine (19) produced significantly more MDR (10) isolates than other sources. Additionally, 19 (29.2%) imipenemresistant E. coli isolates contained 10 MBLs gene, 7(36.8%) isolates contained blaIMP and 3(15.7%) isolates contained blaVIM genes. This study revealed the significant occurrence of MBL producing E. coli isolates in clinical specimens and its association with health risk factors indicating an alarming situation in Malaysia. It demands an appropriate concern to avoid failure of treatments and infection control management.

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supporting

confidence: 90%

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Fazlul¹,

Najnin²,

Yasmin³

et al. 2019

IJPR

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“…It is well known that these genes are present in Shiga toxin-producing E. coli (STEC) and are considered the primary virulence genes responsible for serious public health threats [ 30 ]. In Jordan, high rates of MDR pathogenic E. coli strains have been isolated from patients suffering from urinary tract infections [ 31 ]. These uropathogenic E. coli strains were mostly positive for ST131 and blaCTX-M genes [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Jordan, high rates of MDR pathogenic E. coli strains have been isolated from patients suffering from urinary tract infections [ 31 ]. These uropathogenic E. coli strains were mostly positive for ST131 and blaCTX-M genes [ 31 ]. In another study, high rates of MDR pathogenic E. coli strains, including extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing strains were recovered from different water sources in Jordan [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis

Ismail

Abutarbush

2020

Vet World

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Background and Aim: Mastitis is a common and economically important disease in dairy cattle. It remains one of the most common reasons for the extensive use of antimicrobials in dairy farms leading to the emergence of antimicrobial-resistant pathogens. The aim of this study was to determine the patterns of antimicrobial resistance of Escherichia coli isolates from bovine mastitis and to identify prominent antimicrobial resistance and virulence genes among isolated strains. Materials and Methods: Antimicrobial susceptibility testing against six antibiotic groups, including tetracyclines, aminoglycosides, beta-lactams, macrolides, sulfonamides, and fluoroquinolones was performed using the disk diffusion method. PCR was performed on resistant isolates to detect resistance and virulence genes using commercially available primers. Results: Out of 216 milk samples cultured, 14 samples yielded E. coli isolates. All isolates (100%) were resistant to ampicillin, amoxicillin, procaine penicillin, streptomycin, oxytetracycline, and sulfamethoxazole-trimethoprim. Only one isolate (7%) was sensitive to gentamicin, and all isolates (100%) were sensitive to enrofloxacin and ciprofloxacin. All isolates carried at least one resistance gene against one or more of the major antibiotic groups. All isolates carried the ereA, tetG, tetE, and tetB genes, followed by tetA (93%), ampC (86%), strA (86%), sul1 (78%), tetD (71%), tetC (57%), aadA (57%), and strB (36%). The lowest percentage of isolates carried bla1 (17%) and bla2 (12%) genes, and none of the isolates carried the qnrA gene. Most of the isolates (93%) carried the Shiga toxin 1 virulence gene, followed by complement resistance protein (79%), intimin (64%), Shiga toxin 2 (36%), cytotoxic necrotizing factor (35%), aerotaxis receptor (21%), and type 1 fimbriae (15%). Conclusion: Results of this study indicate that the high percentages of E. coli isolate from bovine mastitis are resistant to two or more of the major antibiotic groups, irrespective of the presence or absence of relevant resistance or virulence genes.

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“…In Jordan, very few studies have addressed the prevalence of ESBL producers in clinical settings. However, blaCTX-M (CTX-M-15, CTX-M-1, and CTX-M-9) was the only ESBL type detected in Enterobacteriaceae notably E. coli and ST131 K. pneumoniae strains (Hayajneh et al, 2015; Aqel et al, 2017; Nairoukh et al, 2018).…”

Section: Distribution Of Multi-drug Resistant Organisms In Humansmentioning

confidence: 99%

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

et al. 2019

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In the last decade, extended-spectrum cephalosporin and carbapenem resistant Gram-negative bacilli (GNB) have been extensively reported in the literature as being disseminated in humans but also in animals and the environment. These resistant organisms often cause treatment challenges due to their wide spectrum of antibiotic resistance. With the emergence of colistin resistance in animals and its subsequent detection in humans, the situation has worsened. Several studies reported the transmission of resistant organisms from animals to humans. Studies from the middle east highlight the spread of resistant organisms in hospitals and to a lesser extent in livestock and the environment. In view of the recent socio-economical conflicts that these countries are facing in addition to the constant population mobilization; we attempt in this review to highlight the gaps of the prevalence of resistance, antibiotic consumption reports, infection control measures and other risk factors contributing in particular to the spread of resistance in these countries. In hospitals, carbapenemases producers appear to be dominant. In contrast, extended spectrum beta lactamases (ESBL) and colistin resistance are becoming a serious problem in animals. This is mainly due to the continuous use of colistin in veterinary medicine even though it is now abandoned in the human sphere. In the environment, despite the small number of reports, ESBL and carbapenemases producers were both detected. This highlights the importance of the latter as a bridge between humans and animals in the transmission chain. In this review, we note that in the majority of the Middle Eastern area, little is known about the level of antibiotic consumption especially in the community and animal farms. Furthermore, some countries are currently facing issues with immigrants, poverty and poor living conditions which has been imposed by the civil war crisis. This all greatly facilitates the dissemination of resistance in all environments. In the one health concept, this work re-emphasizes the need to have global intervention measures to avoid dissemination of antibiotic resistance in humans, animals and the environment in Middle Eastern countries.

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Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients

Cited by 19 publications

References 28 publications

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

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