Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Fazlul, M. K. K.; Najnin, A; Yasmin, Farzana; Rashid, Mamunur; Deepthi, Seelam; Srikumar, C; Rashid, Shah Samiur; Mhm, Nazmul

doi:10.31838/ijpr/2019.11.01.031

Cited by 4 publications

(2 citation statements)

References 52 publications

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“…The findings of the multidrug-resistant level (80%) were consistent with a recent study in Japan by Kusumoto et al ( 62 ). However, 62% of the E. coli strains exhibited MDR in Malaysia ( 63 ). These highlights the rising trend of broad-spectrum MDR in VTEC strains.…”

Section: Discussionmentioning

confidence: 99%

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Khan¹,

Rashid²,

Ramli³

et al. 2022

MJMS

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Background: Diarrhoeagenic verotoxin producing non-O157 Escherichia coli (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children. Methods: Standard microbiological techniques identified a total of 137 non-repeated, clinically significant E. coli isolates. Serological assays discerned non-O157 E. coli serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR). Results: Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157 E. coli. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing E. coli¸ genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates. Conclusion: This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children.

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Section: Discussionmentioning

confidence: 99%

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Khan¹,

Rashid²,

Ramli³

et al. 2022

MJMS

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“…The MBL enzyme can be identified phenotypically in pathogenic bacteria. The recognized techniques are the Modified Hodge Test (MHT), Double-Disc Synergy Test (DDST), Combined Disc Diffusion Test with Imipenem and EDTA (CDDT), and MBL E-Test [13,14]. Since there isn't much information on MBL prevalence in Enugu, this study was carried out to determine how common MBLs are in isolates that are resistant to imipenem (carbapenem) from hospitals and other environmental sources in the city of Enugu.…”

Section: Introductionmentioning

confidence: 99%

Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health

Maduakor,

Eleazar,

Mba

et al. 2024

JAMB

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Background: A potential threat to public health is the rapidly spreading enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae which produce metallo-beta-lactamases (MBL). This study evaluated the prevalence of metallo-beta-lactamase (MBL) from clinical and non-clinical sources in Enugu Metropolis. Methodology: The study was conducted in the Microbiology Laboratory of the University of Nigeria Teaching Hospital, Ituku-Ozalla between October 2020 and July 2021. A total of 150 isolates including 85 and 65 isolates of Escherichia coli and Klebsiella pneumoniae respectively was recovered. Standard microbiology procedures were used to identify and characterize the isolates. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion technique. Phenotypic detection of Metallo-beta-lactamase production was determined using Combined Disk Tests. Results: Imipenem resistance was detected in 22 (25.9%) isolates of E. coli and 18 (27.7%) isolates of K. pneumoniae. Of the 22 strains of E. coli that were imipenem resistant, 8 (9.4%) and 14 (16.5%) were found to be MBL producers and non-MBL respectively. Of the 18 strains of Klebsiella pneumoniae that were imipenem resistant, 10 (15.4%) were MBL producers and 8 (12.3%) were non-MBL producers. The highest prevalence of MBL was recovered from urine sources in both E. coli and K. pneumonieae. All MBL-producing isolates were multidrug resistant. Conclusion: The overall prevalence of MBL in this study was 12.0%. Public health is at risk due to the occurrence of metallo-beta-lactamase. Antimicrobial stewardship and the implementation of infection control strategies are required to halt the spread of these resistant bacteria in the environment. The use of antibiotics should be with utmost prudence.

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Genomic and phenotypic characterization of Acinetobacter colistiniresistens isolated from the feces of a healthy member of the community

Muzahid

Zoqratt

Ten

et al. 2023

Sci Rep

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Acinetobacter species are widely known opportunistic pathogens causing severe community and healthcare-associated infections. One such emerging pathogen, Acinetobacter colistiniresistens, is known to exhibit intrinsic resistance to colistin. We investigated the molecular characteristics of A. colistiniresistens strain C-214, isolated from the fecal sample of a healthy community member, as part of a cohort study being conducted in Segamat, Malaysia. Comparison of the whole genome sequence of C-214 with other A. colistiniresistens sequences retrieved from the NCBI database showed 95% sequence identity or more with many of the genome sequences representing that species. Use of the Galleria mellonella killing assay showed that C-214 was pathogenic in this model infection system. The strain C-214 had a colistin and polymyxin B MIC of 32 and 16 mg/L, respectively. Besides, it was resistant to cefotaxime, amikacin, and tetracycline and showed moderate biofilm-producing ability. Different genes associated with virulence or resistance to major classes of antibiotics were detected. We observed mutations in lpxA/C/D in C-214 and other A. colistiniresistens strains as probable causes of colistin resistance, but the biological effects of these mutations require further investigation. This study provides genomic insights into A. colistiniresistens, a potentially pathogenic bacterium isolated from a community member and notes the public health threat it may pose.

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Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Cited by 4 publications

References 52 publications

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health

Genomic and phenotypic characterization of Acinetobacter colistiniresistens isolated from the feces of a healthy member of the community

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