Molecular Characterization of Metallothionein Genes in Plants.

Yu, Liang; Liu, Jinyuan; Umeda, Masaaki; Uchimiya, Hirofumi

doi:10.5511/plantbiotechnology.15.167

Cited by 7 publications

(7 citation statements)

References 23 publications

(65 reference statements)

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“…Considerable data are available on their structure and expression, and such data have enhanced our understanding of their roles in plant metabolism. Although the exact function(s) of plant MTs remains unclear, it seems that plant MTs are not solely associated with metal ions, but may also be related to other factors such as senescence and non-metal stresses (Yu et al 1998a). …”

Section: Expression Pattern Of Gus Gene In Transgenic Plantsmentioning

confidence: 98%

The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm

Fukuzawa

Umeda-Hara

et al. 2004

Plant Cell Rep

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We generated transgenic tobacco and rice plants harboring a chimeric gene consisting of the 5'-upstream sequence of the rice metallothionein gene (ricMT) fused to the beta-glucuronidase (GUS) gene. The activity and tissue-specific expression of the ricMT promoter were demonstrated in these transgenic plants. In the transgenic rice plants, despite substantial levels of GUS activity in the shoot and root, almost no GUS signal was detected in the endosperm. Thus, the ricMT promoter could be useful in avoiding accumulation of undesired proteins in the seed endosperm.

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Section: Expression Pattern Of Gus Gene In Transgenic Plantsmentioning

confidence: 98%

The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm

Fukuzawa

Umeda-Hara

et al. 2004

Plant Cell Rep

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“…Induction of MTs occurs not only in response to diverse environmental cues but also exhibits characteristic temporal and tissue-speciWc expression patterns. Thus, while the rice ricMT gene shows high expression in stem nodes (Yu et al 1998), the sesame MT-like transcript is found to be abundant in matured seeds (Chyan et al 2005). In pineapple, the expression of PaMT gene is conWned to speciWc stages of fruit development (Moyle et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro

2008

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Prosopis juliflora is a tree species that grows well in heavy metal laden industrial sites and accumulates heavy metals. To understand the possible contribution of metallothioneins (MTs) in heavy metal accumulation in P. juliflora, we isolated and compared the metal binding ability of three different types of MTs (PjMT1-3). Glutathione S-transferase fusions of PjMTs (GSTMT1-3) were purified from Escherichia coli cells grown in the presence of 0.3 mM cadmium, copper or zinc. Analysis of metal bound fusion proteins using atomic absorption spectrometry showed that PjMT1 bound higher levels of all three heavy metals as compared to PjMT2 and PjMT3. A comparative analysis of the genomic regions (including promoter for all three PjMTs) is also presented. All three PjMTs are induced by H(2)O(2) and ABA applications. PjMT1 and PjMT2 are induced by copper and zinc respectively while PjMT3 is induced by copper, zinc and cadmium. Variation in induction of PjMTs in response to metal exposure and their differential binding to metals suggests that each MT has a specific role in P. juliflora. Of the three MTs analyzed, PjMT1 shows maximum heavy metal sequestration and is thus a potential candidate for use in heavy metal phytoremediation.

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“…Type 4 MT sequences are normally expressed in developing seeds (Guo et al 2003). Differential tissue-specific expression of MTs (roots, shoots, seeds, trichomes and fruits) suggests that these proteins, in addition to controlling metal homeostasis, have other important roles during plant development (Zhou et al 2005), senescence (Garcia-Hernandez et al 1998, Yu et al 1998, Navabpour et al 2003, and protection against oxidative stress (Akashi et al 2004, Gisela et al 2004, Wong et al 2004. Plant MTs show a differential response to different heavy metals.…”

Section: Introductionmentioning

confidence: 97%

Characterization of a type 3 metallothionein isolated from Porteresia coarctata

Usha¹,

Keeran²,

Harikrishnan³

et al. 2011

Biologia plant.

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Metallothioneins are involved in detoxification of heavy metals. A cDNA encoding type 3 metallothionein (PcMT3) was isolated from the salt stressed leaf cDNA library of Porteresia coarctata (Roxb.) Tateoka (wild rice) that grows well in the heavy metal laden estuarine soils. The PcMT3 cDNA (581 bp) encodes a protein of 64 amino acids. PcMT3 is highly homologous (82 %) to OsMT-I-3a of rice, but is unique from other type 3 plant MTs due to the presence of an additional glycine residue in the C-terminal domain. Analysis of the 5′ upstream region of PcMT3 showed the presence of cis-acting elements like the CG box and STRE previously reported to be involved in gene expression under heavy metal stress. Southern analysis suggested the presence of more than one copy of PcMT3-like sequences in the P. coarctata genome. Analysis of genomic clone of PcMT3 revealed the presence of two introns. A comparison of the genomic sequence of PcMT3 with closely similar type 3 MTs from rice and mangrove species revealed conservation in the number and position of introns. Transcript profiling for PcMT3 in P. coarctata leaves in the presence of Cd, Cu and Zn showed an increase in transcript accumulation.

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Molecular Characterization of Metallothionein Genes in Plants.

Cited by 7 publications

References 23 publications

The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm

The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm

Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro

Characterization of a type 3 metallothionein isolated from Porteresia coarctata

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