Abstract:We generated transgenic tobacco and rice plants harboring a chimeric gene consisting of the 5'-upstream sequence of the rice metallothionein gene (ricMT) fused to the beta-glucuronidase (GUS) gene. The activity and tissue-specific expression of the ricMT promoter were demonstrated in these transgenic plants. In the transgenic rice plants, despite substantial levels of GUS activity in the shoot and root, almost no GUS signal was detected in the endosperm. Thus, the ricMT promoter could be useful in avoiding acc… Show more
“…These results are consistent with other reports that Cd tolerance and accumulation in sugarcane is mediated by other mechanisms [29]. OsMT1b has greater affinity for Cd and Ni ions compared to Zn and Cu ions, suggesting that OsMT1b may protect rice cells against toxic metals, or play a role in detoxification [23,30]. The over-expression of sheep MTII and maltose binding protein in the cytoplasm of E. coli allowed the bioaccumulation of the heavy metals such as Zn2?, Cu2?…”
Section: Discussionsupporting
confidence: 94%
“…Plant type 1 MTs are more abundantly expressed in roots than leaves, whereas type 2 MTs are expressed primarily in leaves and at a lower level in roots [10,12,14,18,22,23]. Type 3 MTs are expressed in leaves or in ripening fruit [24], whereas type 4 MTs appear to be restricted to developing seeds [25].…”
Metallothioneins (MTs) are cysteine-rich, low molecular weight, metal-binding proteins that are widely distributed in living organisms. Plants produce metal-chelating proteins such as MTs to overcome the toxic effects of heavy metals. We cloned three MT genes from sweetpotato leaves [Ipomoea batatas (L.) Lam.]. The three IbMT genes were classified according to their cysteine residue alignment into type 1 (IbMT1), type 2 (IbMT2), and type 3 (IbMT3). IbMT1 was the most abundantly transcribed MT. It was predominantly expressed in leaves, roots, and callus. IbMT2 transcript was detected only in stems and fibrous roots, whereas IbMT3 was strongly expressed in leaves and stems. The IbMT expression profiles were investigated in plants exposed to heavy metals and abiotic stresses. The levels of IbMT1 expression were strongly elevated in response to Cd and Fe, and moderately higher in response to Cu. The IbMT3 expression pattern in response to heavy metals was similar to that of IbMT1. Exposure to abiotic stresses such as methyl viologen (MV; paraquat), NaCl, polyethylene glycol (PEG), and H2O2 up-regulated IbMT expression; IbMT1 responded strongly to MV and NaCl, whereas IbMT3 was induced by low temperature and PEG. Transgenic Escherichia coli overexpressing IbMT1 protein exhibited results suggest that IbMT could be a useful tool for engineering plants with enhanced tolerance to environmental stresses and heavy metals.
“…These results are consistent with other reports that Cd tolerance and accumulation in sugarcane is mediated by other mechanisms [29]. OsMT1b has greater affinity for Cd and Ni ions compared to Zn and Cu ions, suggesting that OsMT1b may protect rice cells against toxic metals, or play a role in detoxification [23,30]. The over-expression of sheep MTII and maltose binding protein in the cytoplasm of E. coli allowed the bioaccumulation of the heavy metals such as Zn2?, Cu2?…”
Section: Discussionsupporting
confidence: 94%
“…Plant type 1 MTs are more abundantly expressed in roots than leaves, whereas type 2 MTs are expressed primarily in leaves and at a lower level in roots [10,12,14,18,22,23]. Type 3 MTs are expressed in leaves or in ripening fruit [24], whereas type 4 MTs appear to be restricted to developing seeds [25].…”
Metallothioneins (MTs) are cysteine-rich, low molecular weight, metal-binding proteins that are widely distributed in living organisms. Plants produce metal-chelating proteins such as MTs to overcome the toxic effects of heavy metals. We cloned three MT genes from sweetpotato leaves [Ipomoea batatas (L.) Lam.]. The three IbMT genes were classified according to their cysteine residue alignment into type 1 (IbMT1), type 2 (IbMT2), and type 3 (IbMT3). IbMT1 was the most abundantly transcribed MT. It was predominantly expressed in leaves, roots, and callus. IbMT2 transcript was detected only in stems and fibrous roots, whereas IbMT3 was strongly expressed in leaves and stems. The IbMT expression profiles were investigated in plants exposed to heavy metals and abiotic stresses. The levels of IbMT1 expression were strongly elevated in response to Cd and Fe, and moderately higher in response to Cu. The IbMT3 expression pattern in response to heavy metals was similar to that of IbMT1. Exposure to abiotic stresses such as methyl viologen (MV; paraquat), NaCl, polyethylene glycol (PEG), and H2O2 up-regulated IbMT expression; IbMT1 responded strongly to MV and NaCl, whereas IbMT3 was induced by low temperature and PEG. Transgenic Escherichia coli overexpressing IbMT1 protein exhibited results suggest that IbMT could be a useful tool for engineering plants with enhanced tolerance to environmental stresses and heavy metals.
“…During the 2-3 days after sowing, the gene encoding metallothionein was upregulated. Metallothionein is reported to be involved in metal translocation (Fukuzawa et al 2004;Zhou et al 2006), suggesting that metal translocation is important during seed germination. One day after sowing, the genes for amylase and enzymes involved in reduction and were upregulated (Table 2).…”
To investigate the flow of the metal nutrients iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu) during rice seed germination, we performed microarray analysis to examine the expression of genes involved in metal transport. Many kinds of metal transporter genes were strongly expressed and their expression levels changed during rice seed germination. We found that metal transporter genes such as ZIP family has tendency to decrease in their expressions during seed germination. Furthermore, imaging of the distribution of elements (Fe, Mn, Zn, and Cu) was carried out using Synchrotronbased X-ray microfluorescence at the Super Photon ring-8 GeV (SPring-8) facility. The change in the distribution of each element in the seeds following germination was observed by in vivo monitoring. Iron, Mn, Zn, and Cu accumulated in the endosperm and embryos of rice seeds, and their distribution changed during rice seed germination. The change in the patterns of mineral localization during germination was different among the elements observed.
“…It has thus been suggested that these MRE or MRE-like sequences may be involved in heavy metal responsiveness. However, to our knowledge, to date there is no evidence showing MRE or MRE-like sequence confers heavy metal responsiveness to these plant genes (Evans et al, 1990;Whitelaw et al, 1997;Fukuzawa et al, 2004;Perry et al, 2005). Thus, MRE-like sequences in plants may be involved in other forms of regulation.…”
Section: The Function Of the Mre-like Sequence In Plantsmentioning
The bean (Phaseolus vulgaris) stress-related gene number 2 (PvSR2) gene responds to heavy metals but not to other forms of environmental stresses. To elucidate its heavy metal-regulatory mechanism at the transcriptional level, we isolated and characterized the promoter region (21623/148) of PvSR2. Deletions from the 5# end revealed that a sequence between 2222 and 2147 relative to the transcriptional start site was sufficient for heavy metal-specific induction of the promoter region of PvSR2. Detailed analysis of this 76-bp fragment indicated that heavy metal-responsive elements were localized in two regions (2222/2188 and 2187/2147), each of which could separately confer heavy metal-responsive expression on the b-glucuronidase gene in the context of a minimal cauliflower mosaic virus 35S promoter. Region I (2222/2188) contains a motif (metalregulatory element-like sequence) similar to the consensus metal-regulatory element of the animal metallothionein gene, and mutation of this motif eliminated the heavy metal-inducible function of region I. Region II (2187/2147) had no similarity to previously identified cis-acting elements involved in heavy metal induction, suggesting the presence of a novel heavy metalresponsive element. Transformed tobacco (Nicotiana tabacum) seedlings expressing b-glucuronidase under control of the PvSR2 promoter region (2687/148) showed heavy metal-specific responsive activity that depended on the type and concentration of the heavy metal and the type of organ. These findings further our understanding of the regulation of PvSR2 expression and provide a new heavy-metal-inducible promoter system in transgenic plants.In plants, a number of heavy metals are essential for normal growth as cofactors and as structural and catalytic components of proteins and enzymes. These micronutrients, as well as nonessential heavy metals such as cadmium, are toxic at high levels. Plants have evolved a suite of mechanisms that control and respond to the uptake and accumulation of both essential and nonessential heavy metals. These mechanisms include the chelation and sequestration of heavy metals by particular ligands including small peptides, organic acids, and amino acids, which bind free metal ions. They contribute to metal detoxification by buffering cytosolic metal ions (Clemens, 2001). The two best-characterized heavy metal-binding ligands in plant cells are the phytochelatins (PCs) and metallothioneins (MTs; for review, see Cobbett and Goldsborough, 2002). MTs are Cys-rich polypeptides encoded by a family of genes. In contrast, PCs are a family of enzymatically synthesized Cys-rich peptides. The induction of PC synthesis is based on the posttranscriptional activation of preformed PC synthase and is not under transcriptional control.Several heavy metal-inducible genes have been reported in plants (Hagen et al., 1988;Lescure et al., 1991;Berna and Bernier, 1999), but surprisingly, little is known about the transcriptional regulation of gene expression in response to heavy metals. In particular, the cis-...
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