Molecular characterization of metallo β-lactamase producing multidrug resistant Pseudomonas aeruginosa from various clinical samples

Raman, K.; Saranathan, Rajagopalan; Nair, Sreejayan; Prashanth, K; Chandrakesan, Sheela Devi

doi:10.4103/0377-4929.142670

Cited by 18 publications

(18 citation statements)

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“…All the isolates showed 100% sensitive to polymyxin B and colistin. [11] In this study, we have observed complete resistance to all the antibiotics tested except imipenem which showed 85% isolates were resistance, which is in concordance with other earlier studies, however, we did not include colistin in our study. A report by Aliskan et al, with 1071 MDRPA, reported resistance to imipenem (22.5%) and meropenem (31%).…”

Section: Discussionsupporting

confidence: 91%

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Raj¹,

Pa²,

Jain³

2018

Natl J Physiol Pharm Pharmacol

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Background: In recent decades, Pseudomonas aeruginosa has emerged as a multidrug-resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents by uses distinctive resistant mechanisms to all the available antibiotics, which include metallo-β-lactamases (MBL) production, extended spectrum β-lactamase production, Amp C production, decreased permeability, altered penicillin binding proteins and rarely, and over expression of efflux pumps. Aims and Objectives: The aim of the study was to know the presence of IMP gene among P. aeruginosa isolates and to determine the carbapenem resistance mechanisms in carbapenem-resistant isolates of P. aeruginosa collected from sputum, blood, urine, pus, in Chennai, India. Materials and Methods: A total of 20 of non-repetitive clinical isolates of P. aeruginosa were collected and processed for biochemical tests and confirmed. Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. P. aeruginosa isolates were detected for the presence of bla IMP gene by polymerase chain reaction analysis. Results: Of the 20 clinical isolates of P. aeruginosa, 9/20 (45%) isolates were from sputum, 5/20 (25%) from blood, 3/20 (15%) from urine, and 3/20 (15%) from pus. Only 2/20 (10%) isolates showed sensitivity to imipenem. Other than that, for all other antibiotics isolates showed complete resistance 20/20 (100%). 17/20 (85%) clinical isolate of P. aeruginosa was found to possess bla IMP gene. Conclusion: The early detection of MBL-producing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multidrug-resistant strains. However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened.

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Section: Discussionsupporting

confidence: 91%

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Raj¹,

Pa²,

Jain³

2018

Natl J Physiol Pharm Pharmacol

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“…P. aeruginosa is intrinsically resistant to several antimicrobial agents and has the capacity to acquire further resistance mechanisms (Ramakrishnan et al, 2014). The frequency of multidrug-resistant and pan-drug resistant strains of P. aeruginosa is high in ICUs and increases mortality, morbidity, and hospital costs (Hong et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice

Pan

Tan

et al. 2016

Front. Cell. Infect. Microbiol.

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The host acquired immune response, especially the humoral immunity, plays key roles in preventing bacterial pneumonia in the lung. Our previous research demonstrated that interleukin 17-producing γδ T cells (IL17-γδ T cells) have a protective effect on the early innate immune response during acute pulmonary Pseudomonas aeruginosa infection. However, whether IL17-γδ T cells also play a role in humoral immunity is unknown. In this study, an acute pulmonary P. aeruginosa infection model was established in wild-type and γδ TCR−/− C57BL/6 mice. The expression of IL-17 on γδ T cells isolated from infected lung tissues increased rapidly and peaked at day 7 after acute infection with P. aeruginosa. Compared with wild-type infected mice, the levels of total immunoglobulins including IgA, IgG, and IgM in the serum and BALF were significantly decreased in γδ TCR−/− mice, with the exception of IgM in the BALF. Moreover, CD69 expression in B cells from the lungs and spleen and the level of BAFF in the plasma were also decreased in γδ TCR−/− mice. IL17-γδ T cell transfusion significantly improved the production of immunoglobulins, B cell activation and BAFF levels in γδ TCR−/− mice compared with γδ TCR−/− mice without transfusion; this effect was blocked when cells were pretreated with an IL-17 antibody. Together, these data demonstrate that IL17-γδ T cells are involved in CD19+ B cell activation and the production of immunoglobulins during acute pulmonary P. aeruginosa infection. Thus, we conclude that IL17-γδ T cells may facilitate the elimination of bacteria and improve survival through not only innate immunity but also humoral immunity.

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“…Yoğun bakım hastalarında ampirik tedavide karbapenemler, florokinolonlar ve üçüncü kuşak sefalosporinlerin kullanılması Pseudomonas gibi dirençli bakterilerin etken olduğu enfeksiyonların artmasına neden olmaktadır [4,14]. Özellikle yoğun bakım ünitelerinde çoklu ilaca dirençli P. aeuginosa suşlarının dünya genelinde artmakta olduğu bildirilmektedir [10,15].…”

Section: Gereç Ve Yöntemunclassified

“…Makrolidler, tetrasiklin, rifampin ve sefiksime karşı ise yüksek oranda direnç gösterir. Hızlı direnç gelişiminde rol oynayan mekanizmalar arasında beta-laktamaz üretimi, dış membran geçirgenliğinde azalma ve aktif pompalama sistemleri sayılabilir [3][4][5]. P. aeruginosa çoklu antibiyotik direnci gösterebilmesi ve kullanılan antimikrobiyal ajanlara karşı hızlı direnç geliştirebilmesi nedeniyle özelikle yoğun bakımda izlenen hastaların tedavilerinde sorunlara neden olmaktadır [6,7].…”

Section: Introductionunclassified

Assessment of <i>Pseudomonas aeruginosa</i> resistance profiles in intensive care units: Five-year outcomes

et al. 2017

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Pseudomonas cinsi bakteriler doğada yaygın olarak bulunan, fırsatçı enfeksiyonlar ve hastane enfeksiyonlarına yol açan non-fermantatif Gram negatif basillerdir. Hastanelerde özellikle yoğun bakımlarda izole edilen bu etken mekanik ventilatöre bağlı, kemoterapi ve antibiyotik tedavisi alan bağışıklık sistemi baskılanmış hastalarda önemli morbidite ve mortalite nedenidir. Bu çalışmanın amacı yoğun bakım ünitelerinden izole edilen Pseudomonas aeruginosa (P.aeruginosa) suşlarında antibiyotik duyarlılık profilinin belirlenmesidir.

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Molecular characterization of metallo β-lactamase producing multidrug resistant Pseudomonas aeruginosa from various clinical samples

Cited by 18 publications

References 1 publication

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa

IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice

Assessment of <i>Pseudomonas aeruginosa</i> resistance profiles in intensive care units: Five-year outcomes

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