Molecular characterization of Kaposi's sarcoma associated herpesvirus (KSHV) from patients with AIDS-associated Kaposi's sarcoma in São Paulo, Brazil

Nascimento, Maria Cláudia; Wilder, Natalie; Pannuti, Cláudio Sérgio; Weiss, Helen A.; Mayaud, Philippe

doi:10.1016/j.jcv.2004.09.026

Cited by 27 publications

(45 citation statements)

References 36 publications

(54 reference statements)

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“…[2,12,13] Such inconsistencies may be partially attributed to the lack of a standard method or protocol for HHV-8 genotyping, especially regarding the choice of targets in the HHV-8 genome and a more complete coverage of HHV-8 variants.…”

Section: Introductionmentioning

confidence: 99%

“…Investigation of biomarkers potentially useful for KS prognosis based on different loci of genome [9,10,12,14,15,18] or whole-genome data [16] has advanced parallel to the molecular characterization of a large number of HHV-8 strains infecting patients from different clinical and epidemiological forms of KS. [9,10,12,14,15] For example, several short targets of HHV-8 from AIDS-KS lesions were selected to identify distinctive molecular pattern, but with restricted coverage of the repertoire of HHV-8 genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Tozetto‐Mendoza¹,

Ibrahim²,

Tateno

et al. 2016

Medicine

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AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Tozetto‐Mendoza¹,

Ibrahim²,

Tateno

et al. 2016

Medicine

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“…In 2004 Nascimento et al 36 reported the molecular characterization of 33 AIDS-related KS specimens from Sao Paulo. Nineteen patients reported their ethnicity to be Caucasian, nine reported African descent, four reported mixed origin, and one patient was of Amazonian descent.…”

Section: Brazilmentioning

confidence: 99%

Epidemiology and clinical characteristics of classic Kaposi's sarcoma, seroprevalence, and variants of human herpesvirus 8 in South America: A critical review of an old disease

Mohanna

Maco

Bravo

et al. 2005

International Journal of Infectious Diseases

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Classic KS in South America has a very similar clinical presentation but not the same as the classic KS variety described in the Mediterranean. Initial seroprevalence studies performed in the general population and in blood donors showed low seroprevalence of HHV-8, whereas high seroprevalence rates were seen in Amerindian population. The existing serological assays, nonetheless, need to be further refined, and new assays need to be developed. Finally, the key to understanding the precise molecular epidemiology and phylogenetic distribution of HHV-8 in South America would be to perform more subtyping of classic KS cases.

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“…No Brasil foram relatados os subtipos A, B e C no estado de São Paulo (Biggar et al, 2000;Caterino-de-Araujo et al, 2003) e o subtipo E em índios da região Amazônica (Nascimento et al, 2005;Ishak et al, 2007).…”

Section: Biologia Do Herpesvírus 8 Humanounclassified

Avaliação da resposta tecidual "in situ" do fenótipo, da expressão de HHV-8/LANA e de citocinas em lesões cutâneas de sarcoma de Kaposi clássico e sarcoma de Kaposi associado à AIDS na era pré e pós-terapia anti-retroviral combinada

Guedes¹

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... 116Anexo A -Aprovação -peculiar nas lesões de SK. Os macrófagos CD68+ estiveram aumentados nas lesões de SKC quando comparados com as lesões de SK-AIDS, mas similares com aqueles encontrados em lesões de SK-AIDS/HAART. Dados semelhantes foram encontrados nas células de Langerhans epidérmicas nesses grupos, sugerindo uma recuperação immune parcial através da HAART. O número aumentado de células expressando IFN-γ em lesões de SKC e SK-AIDS/HAART quando comparado com SK-AIDS sugere essa citocina como um indicador de resposta imune mais eficaz. A expressão aumentada de IL-1β nas lesões de SKC e SK-AIDS/HAART poderia estar relacionada ao seu efeito anti-tumoral. A expressão de TNF-α, IL-4 e IL-6 foram similares entre as lesões de SK avaliadas. Através de dupla marcação, a identificação nuclear de HHV-8 em DD FXIIIa+ sugere esse tipo celular como alvo para infecção por HHV-8. As lesões de SKC apresentaram número aumentado de células com expressão de HHV-8 quando comparado com os grupos de SK-AIDS, independente da HAART. Nosso estudo mostra que existiu uma recuperação da resposta immune local nas lesões de SK-AIDS/HAART e que a severidade clínica do SK não pode estar diretamente associada com a densidade aumentada de células infectadas pelo HHV-8 no tecido.

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Molecular characterization of Kaposi's sarcoma associated herpesvirus (KSHV) from patients with AIDS-associated Kaposi's sarcoma in São Paulo, Brazil

Cited by 27 publications

References 36 publications

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Epidemiology and clinical characteristics of classic Kaposi's sarcoma, seroprevalence, and variants of human herpesvirus 8 in South America: A critical review of an old disease

Avaliação da resposta tecidual "in situ" do fenótipo, da expressão de HHV-8/LANA e de citocinas em lesões cutâneas de sarcoma de Kaposi clássico e sarcoma de Kaposi associado à AIDS na era pré e pós-terapia anti-retroviral combinada

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