Molecular characterization of <i>Anisakis pegreffii</i> larvae in Pacific cod in Japan

Abe, Niichiro; Ohya, N.; Yanagiguchi, R.

doi:10.1079/joh2005290

Cited by 51 publications

(23 citation statements)

References 13 publications

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“…Though earlier morphological studies indicated a worldwide distribution for the marine fish nematode Anisakis simplex, recent molecular studies demonstrated that the morphotype consists of three cryptic or sibling species, A. simplex (sensu stricto), A. pegreffii and A. simplex C, having similar morphotypes but different genotypes and being separated by different host preferences and geographical distributions (Abe et al 2005;Marques et al 2006). Anisakis simplex (s.s.) is cosmopolitan and occurs especially in the North Atlantic and the Pacific Ocean, while A. pegreffi is most frequent in the Mediterranean Sea and the southern hemisphere and A. simplex C is most frequent at the Pacific coast of Canada and in the southern hemisphere (Marques et al 2006;Mattiucci et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in the trypanorhynch cestode Tentacularia coryphaenae Bosc, 1797: evidence for a cosmopolitan distribution and low host specificity in the teleost intermediate host

2007

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Partial large subunit (28S) rRNA gene (LSU) sequences were studied from Tentacularia coryphaenae (Cestoda: Trypanorhyncha) plerocercoids from the southern Java coast, Indonesia, collected from two different localities and five different host species. The teleost hosts belonged to four fish families with an overlapping depth range of 0-885 m. The LSU sequences were identical, demonstrating that all specimens belonged to the same species. They also corresponded to a sequence of T. coryphaenae from the Blue shark Prionace glauca in the North Atlantic, giving genetic evidence for the cosmopolitan distribution of the species. A 1,851 bp region of mitochondrial (mt) DNA (coding for partial cytochrome c oxidase subunit I (cox1), complete trnT and partial 16S ribosomal RNA) showed a very low level of intra-specific variation of 1%. Pairwise comparisons of published sequences for partial LSU rDNA and the same region of mtDNA demonstrated that the same regions varied by 8% in the mtDNA for two genotypes (G1 and G4) of Echinococcus granulosus (order Cyclophyllidea), at 16% in newly sequenced Kotorella pronosoma from the same trypanorhynch family and at 23% in Grillotia pristiophori from a different superfamily. The high genetic homogeneity in T. coryphaenae is explained by a constant gene flow between different regions and hosts along the Indonesian coast caused by extensive migrations of the second intermediate/paratenic and also the final hosts. Implications for the zoogeographical distribution, host specificity of the species and future research are discussed.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity in the trypanorhynch cestode Tentacularia coryphaenae Bosc, 1797: evidence for a cosmopolitan distribution and low host specificity in the teleost intermediate host

2007

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“…Using a molecular polyphasic approach such as PCR-RFLP, sequencing of nuclear and mitochondrial markers and phylogenetic analysis, we were able to specifically identify A. pegreffii in M. merluccius and L. piscatorius from Adriatic Sea waters. The unequivocal identification of A. pegreffii by using the entire ITS was possible for the specific restriction pattern obtained with the enzyme Hinf I and the diagnostic nucleotides C and C at alignment positions 475 and 491 peculiar for A. pegreffii (Abollo et al, 2003;Abe et al, 2005;Ceballos-Mendiola et al, 2010). These findings clearly demonstrated that the heterozygote pattern observed with Hinf I enzyme is due to an incomplete digestion of the amplified DNA.…”

Section: Discussionmentioning

confidence: 68%

A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea

Luca¹,

Vovlas

Troccoli³

et al. 2013

Helminthologia

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SummaryIn this study we investigated the morphometric and molecular characterization of a liver encapsulated third-stage larval population of Anisakis spp. infecting Merluccius merluccius and Lophius piscatorius caught in the Adriatic Sea waters (southern Italy). A polyphasic approach based on PCR-RFLP profiles of the ITS region, mitochondrial COI (cytochrome c oxidase subunit 1), sequencing and molecular phylogeny of ITS and mitochondrial COI was used to identify Anisakis larvae collected from fish samples. PCR-RFLP analysis showed three banding pattern corresponding to the peculiar pattern of A. pegreffii. Sequence data from ribosomal ITS and mitochondrial COI were analysed by Neighbour Joining, Minimum Evolution and Maximum Parsimony methods to evaluate the phylogenetic relationships among A. simplex sensu lato. The phylogenetic trees obtained for both ITS and COI revealed the existence of three distinct clades for A. simplex sensu sricto, A. simplex C and A. pegreffii and the sequences obtained in this study clearly clustered together with A. pegreffii sequences present in the database. Histopathological observations of anisakid nematode specimens detected on the liver surface of M. merluccius are illustrated. Encapsulated specimens of the L3 stage of the nematode were similar in size and morphometry to those found into the peritoneal cavity. Anisakis larvae encapsulated on the liver surface within dense and pearl coloured envelops caused host hepatic tissue necrosis, large cavities and oedematous liver spots to the host.

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“…The presence of larval anisakids in the body muscle of fish is more of human food safety concern than those infecting the non-edible parts (visceral organs and other non-consumable parts) [30]. The incidences of human anisakiasis due to A. simplex sensu stricto (s.s.) and A. pegreffii have been well documented from Japan [31][32][33] and Mediterranean [34][35], respectively. Moreover, though past studies have evaluated the migration of A. simplex complex and unidentified Anisakis larvae into the body muscle of both live and dead fish [36][37][38][39][40][41][42], these parasites were not clearly identified to sibling species level (i.e., if A. simplex s.s., A. pegreffii or A. berlandii).…”

Section: Parasitic Threat To Food Safetymentioning

confidence: 99%

Updates on Aquatic Parasites in Fisheries: Implications to Food Safety, Food Security and Environmental Protection

Quiazon¹

2015

J Coast Zone Manag

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Journal of Coastal Zone Management AbstractCheap protein sources from fishery products come from both capture fishery and aquaculture industries. Despite the available technologies that help increase wild catch and aquaculture production, our food security is being threatened by several factors including parasitic infections. Zoonotic parasites infecting our fishery products are one of the several concerns for our food safety. Among these include the infections with the nematode Anisakis in marine fishes and cephalopods causing human anisakiasis and/or allergy-associated health risks, the nematode Gnathostoma causing gnathostomiasis, and food poisoning due to the myxozoan infection of the genus Kudoa. On the other hand, the increasing human population, dwindling fish catch from the wild, degradation of aquatic environment, and declining or slow growth of aquaculture sector due to parasitic diseases are all posing global threats to the security and sustainability of fish supplies. The wild fish populations are affected by the parasitic diseases that directly and indirectly affect fish reproduction, growth, and survival, whereas intensifications of aquaculture operations cause fish health problems associated to parasitic diseases resulting to decline in production. Despite these negative impacts of parasites, there are several parasite groups that are used as biological indicators for food chain structure, heavy metal contamination, environmental pollutions and fish stock assessment (i.e., nematodes Anisakis, Hysterothylacium, Anguillicola, Spirophilometra, Raphidascaris, and Philometra; acanthocephalans Pomphorhynchus, Serrasentis, and Acanthocephalus; cestodes Bothriocephalus, Monobothrium, and Ligula; monogenean Pseudorhabdosynochus; and digenean Didymodiclinus), as well as reducer of heavy metal accumulation in the body of their host fish (i.e., acanthocephalans Pomphorhynchus and Acanthocephalus). The use of these parasites for proper management of fishery resources can be of help in addressing food safety, fish security, and food sustainability, while at the same time managing our fishery resources. As we are addressing these global issues, these parasites that we are considering as threats can be of useful value to attain sustainable development.

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Molecular characterization of Anisakis pegreffii larvae in Pacific cod in Japan

Cited by 51 publications

References 13 publications

Genetic diversity in the trypanorhynch cestode Tentacularia coryphaenae Bosc, 1797: evidence for a cosmopolitan distribution and low host specificity in the teleost intermediate host

Genetic diversity in the trypanorhynch cestode Tentacularia coryphaenae Bosc, 1797: evidence for a cosmopolitan distribution and low host specificity in the teleost intermediate host

A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea

Updates on Aquatic Parasites in Fisheries: Implications to Food Safety, Food Security and Environmental Protection

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