Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014

Kenmoe, Sébastien; Vernet, Marie‐Astrid; Goff, Jérôme Le; Penlap, Véronique; Vabret, Astrid; Njouom, Richard

doi:10.1186/s12985-018-1064-x

Cited by 14 publications

(26 citation statements)

References 34 publications

(36 reference statements)

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“…Most of the HAdV‐positive infections detected in this study were HAdV‐C followed by HAdV‐B. A report from Cameroon found that HAdV‐B infections were more common and severe than HAdV‐C 33 . On the contrary, studies conducted in Egypt and Senegal were consistent with our findings showing a predominance of HAdV‐C 10,34 .…”

Section: Discussionsupporting

confidence: 91%

“…This study has discovered that HAdV seems to increase the risk of low oxygen saturation and the duration of oxygen support in the pediatric environment which is in line with other reports showing that HAdV is associated with severe clinical manifestations 30,31 . In Africa, HAdV was associated with influenza‐like illnesses in several countries like Morocco, Egypt, Cameroon, and Senegal 13,32‐34 . However, the severity of respiratory infections was variable.…”

Section: Discussionsupporting

confidence: 89%

“…However, the severity of respiratory infections was variable. In Cameroon, HAdV was not found to be associated with severity 33 . Conversely, in Senegal, HAdV infection was statistically correlated with myalgia, cough, diarrhea, headache, rhinitis, and pharyngitis 34 .…”

Section: Discussionmentioning

confidence: 83%

“…The difference in the sensitivity of qPCR used for HAdV‐detection could justify these results. Indeed, Kenmoe et al 33 found a significantly low viral load of non‐typed samples with standard PCR compared to successfully typed samples (mean Ct‐values of 28.9 vs 15.4, respectively). In this study, the mean Ct‐values for non‐typed samples were 34.4 against 24.6 for successfully typed samples.…”

Section: Discussionmentioning

confidence: 99%

“…30,31 In Africa, HAdV was associated with influenza-like illnesses in several countries like Morocco, Egypt, Cameroon, and Senegal. 13,[32][33][34] However, the severity of respiratory infections was variable. In Cameroon, HAdV was not found to be associated with severity.…”

Section: Type Identification Of Hadvs and Phylogenetic Analysismentioning

confidence: 99%

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Human adenoviruses associated with respiratory illness in neonates, infants, and children in the Sousse area of Tunisia

Brini

Guerrero

Ezzine

et al. 2020

Journal of Medical Virology

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Background The human Adenovirus (HAdV) is a common agent of acute respiratory infections (ARIs). Its clinical impact in immunocompetent children and in the context of coinfections remains unclear in Tunisia. Material and methods HAdV‐ARIs were studied in hospitalized patients from birth to the age of 5 years from 2013 to 2014. Clinical and demographic characteristics, coinfections, and molecular characterization of HAdV were established. Results HAdV‐positivity was detected in 114/583 specimens (19.6%) including 6.1% single infections and 93.9% coinfections. Adenoviral coinfections mostly comprised human Rhinovirus (50.9%), Streptococcus pneumoniae (34.2%), human Respiratory Syncytial virus A/B (29.8%), and human Coronaviruses (21.9%). HAdV infection was predominant in the pediatric population (25.0% vs 10.0% in neonates, P < .001) and peaked in February 2014 (21.1%). HAdV severity of pediatric cases is characterized by low saturation of oxygen (<94%, 33.8%, P = .05) and long duration of oxygen support (≥5 days, 32.7%, P = .02). Severe HAdV infections were described with S. pneumoniae coinfection, which seemed to increase the risk of death. HAdV genotyping identified HAdV‐C as the most common species. Severe ARIs were observed in all HAdV‐identified types. Phylogenetic analysis revealed that sequences were variable suggesting the circulation of different HAdV strains sharing more similarities to strains circulating in Europe or Asia than those from Africa. Conclusion This first molecular study of HAdV in Tunisia demonstrated that it has an important role in severe ARIs with HAdV‐C being the most common species. S. pneumoniae codetection seems to increase the severity of HAdV‐ARIs.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Type Identification Of Hadvs and Phylogenetic Analysismentioning

confidence: 99%

See 3 more Smart Citations

Human adenoviruses associated with respiratory illness in neonates, infants, and children in the Sousse area of Tunisia

Brini

Guerrero

Ezzine

et al. 2020

Journal of Medical Virology

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Seroprevalence and molecular characterization of human respiratory syncytial virus and human adenovirus among children in Ado‐Ekiti, Nigeria

Idris

Kolawole

2021

Journal of Medical Virology

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In children, the respiratory syncytial virus and adenovirus majorly cause acute respiratory infections. The study evaluated the seroprevalence and conducted the molecular characterization of human respiratory syncytial virus (HRSV) and human adenovirus (HAdV) among children. Venous blood, nasopharyngeal, and oropharyngeal swabs were collected from children presenting with acute respiratory infections in a tertiary health facility in Ado-Ekiti, Nigeria. A serological investigation was carried out on the sera samples for the detection of anti-HRSV immunoglobulin M (IgM), anti-HRSV IgG, anti-HAdV IgM, and anti-HAdV IgG using ELISA (enzyme-linked immunosorbent assay) kits. The amplification and sequencing of HRSV and HAdV were carried out using specific primer pairs that targeted the glycoprotein (G) gene of HRSV and the hexon gene of HAdV, respectively.The seroprevalence of HRSV IgG and IgM was 73% and 7.5%, respectively, while the seroprevalence of HAdV IgG and IgM was 98.5% and 8.5%, respectively. The age of enrolled children, presence of fever, and cough were associated (p < 0.05) with the infection. HRSV subtype B (HRSV-B) (13.3%), and species of HAdV (Mastadenovirus B and C) (11.7%) were detected among the studied population. There was no viral coinfection with both HRSV and HAdV. In infancy and early childhood, HRSV-B, HAdV species B and C are common etiologic agents of respiratory infections as reported in this study. Further studies on molecular characterization of respiratory tract viruses including circulating respiratory syncytial virus and adenovirus are hereby advocated.

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Cross-infection of adenovirus among medical staff: A warning from the intensive care unit in a tertiary care teaching hospital in China

Dai

Tan

et al. 2020

International Journal of Infectious Diseases

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In 2019, a small HAdV55-associated outbreak of adenovirus infection occurred among the intensive care unit (ICU) staff in Xiangya Hospital of Central South University in Hunan Province, China, during the treatment of a patient. Objective: To investigate the characteristics of a nosocomial adenovirus outbreak in an ICU. Methods: We evaluated all the patients treated and the medical staff working in the ICU from August 1 to September 4, 2019. We further performed an epidemiological and molecular analysis for this outbreak from patient to healthcare workers and between healthcare workers. After the outbreak, we adopted exposure prevention and droplet prevention measures based on standard precautions. Measurements and main results: Between August 1 and August 27, 2019, 27 cases of human adenovirus cross-infection were reported in our institution. Among the cases, eleven were doctors (41%), eleven were nurses (41%), three were respiratory therapists (11%), and two were caregivers (7%). The attack rate was 28.4%, and the fatality rate was 0. The results showed that contact with the index case, lack of hand hygiene or gloving adherence were risk factors for infection after adenovirus exposure. After taking specific precautions, no new cases of infection have appeared since August 27. Conclusions: Our results show that HAdV55 in a single patient had strong transmission potential in an intensive care unit with adequate facilities and standardized operation. We provide convincing evidence indicating that attention could be highlighted on the role of standard and specific precautions for controlling the spread of adenovirus in ICUs.

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Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014

Cited by 14 publications

References 34 publications

Human adenoviruses associated with respiratory illness in neonates, infants, and children in the Sousse area of Tunisia

Human adenoviruses associated with respiratory illness in neonates, infants, and children in the Sousse area of Tunisia

Seroprevalence and molecular characterization of human respiratory syncytial virus and human adenovirus among children in Ado‐Ekiti, Nigeria

Cross-infection of adenovirus among medical staff: A warning from the intensive care unit in a tertiary care teaching hospital in China

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