Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction

Andrade, Paula Durante; Russo, Joice de Souza; Gouveia, Jéssica Baliero; Costa, Caetano da; Oliveira, Ketti Gleyzer de; Gianetti, Michelli; Anjos, Emanuel Borges Vítor; Pavan, Tycha Bianca Sabaini; Martins, Mariana Furquim da Silva; Franco, Josiele; Costa, Maria Laura; Nomura, Marcelo Luís; Levy, Carlos Emílio; Júnior, Renato Passini; Costa, Sandra Cecı́lia Botelho

doi:10.5935/medicalexpress.2017.04.06

Cited by 3 publications

(10 citation statements)

References 30 publications

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“…[32] Both studies were conducted in Rio de Janeiro. In São Paulo, serotype Ia was the most abundant followed by V and II in a study conducted in 2015-2016 [54], suggesting that the increased prevalence in serotype Ia may be related with a period of time. In Paraná, serotype Ia was also the most prevalent; however, there was a surprisingly high number of serotype IV strains [53].…”

Section: Serotypesmentioning

confidence: 96%

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Streptococcus agalactiae in pregnant women in Brazil: prevalence, serotypes, and antibiotic resistance

et al. 2019

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Brazilian data for maternal GBS colonization shows different prevalence rates. This conflicting data may be related to the absence of an official recommendation from the Federal Brazilian Health Authorities describing guidelines and protocols to perform GBS screening in pregnant women, in both public and private clinics. In the present review, we evaluated published reports addressing the prevalence of GBS in different regions of the country, methods used, and, when available, information regarding antibiotic resistance and serological typing of clinical isolates. According to this review, GBS prevalence in pregnant women in Brazil ranged from 4.2 to 28.4%, in the last 10 years. Serotype Ia was the most prevalent. The highest antibiotic resistance rates were found for tetarcycline, although its use to treat GBS infections is not common. Our results also show high resistance rates to clindamycin and erythromycin, which are commonly used as an alternative to penicillin in GBS infecctions. The increased antibiotic resistance, variations in serotype distribution, and high GBS prevalences need to be further investigated. Based on the present situation, recommendations regarding GBS surveillance in the country were raised and may improve our strategies for preventing neonatal infections.

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Section: Serotypesmentioning

confidence: 96%

“…There is a divergence in serotype distribution among pregnant women from different locations in Brazil [32,[51][52][53][54]. Few studies evaluated GBS serotypes, and from 2008 to 2017, most of them were performed in the Southeast Region (Fig.…”

Section: Serotypesmentioning

confidence: 99%

Streptococcus agalactiae in pregnant women in Brazil: prevalence, serotypes, and antibiotic resistance

et al. 2019

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“…However, there are concerns about resistance to antibiotics, as it may involve prophylaxis and inadequate treatment 25 . In studies conducted in Brazil, for example, resistance to clindamycin and/or erythromycin was found in isolates of serotypes Ia, Ib, II, III, V, VIII, and IX 4,17,18,31,33,45 . As a result of the increase in this resistance, a study reinforced the more recent guidance of the CDC (2010), which recommends carrying out susceptibility tests in the samples of GBS before treating colonized pregnant women 15 …”

Section: Discussionmentioning

confidence: 96%

“…3,14,15 Serotypes can be determined using techniques such as latex agglutination and polymerase chain reaction (PCR). 16,17 In the first technique, specific antibodies, available in reliable and easy-to-use commercial kits, are used for CPSs, whereas the PCR is based on the amplification of nucleotide sequence of the genes responsible for the capsular constituents. PCR has greater sensitivity and specificity in the identification of GBS than latex agglutination assay.…”

Section: Introductionmentioning

confidence: 99%

Distribution and Prevalence of Serotypes of Group B Streptococcus Isolated from Pregnant Women in 30 Countries: A Systematic Review

Silva

Oliveira

et al. 2023

Maternal Fetal Med

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Objective: This review aimed to compile scientific data on the distribution and prevalence of group B Streptococcus (GBS) serotypes isolated from pregnant women across 30 countries from 2010 to 2019.Methods: This was a systematic review that addresses the distribution and prevalence of GBS in pregnant women. The search included studies published between January 2010 and December 2019 in PubMed, Virtual Health Library (BVS), ScienceDirect, Scientific Electronic Library Online (SciELO), and LILACS databases. We also surveyed relevant articles published in English, Spanish, and Portuguese between February and April 2020. Original articles, communication, short report, theses, and dissertations were included. The prevalence of GBS colonization, method for capsular serotyping, antimicrobial resistance, distribution and prevalence of serotypes were extracted from each study.Results: In all, 795 publications were identified. After applying the eligibility criteria, 48 articles were included for the final systematic analysis; most articles were from Asia and were published during the years 2014 to 2017. For the identification of serotypes, most studies used the polymerase chain reaction technique. There were records of all 10 GBS serotypes, namely, Ia, Ib, and II-IX, among the countries analyzed. GBS susceptibility and resistance to antibiotics were addressed in 37.5% of the publications analysed. Conclusion:This review showed that GBS serotypes are distributed differently in the 30 analyzed countries, with serotypes Ia, Ib, and II to V being the most prevalent. Furthermore, our results highlighted the relationship of GBS with maternal colonization, implications for neonates, and antibiotic resistance.

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“…The identification of E. coli through PCR in placental tissues of pregnant women with risk factors for neonatal sepsis enhances the inclusion of the test in the Brazilian National Health System (SUS), mainly for women included in the risk group of sepsis. The test possesses accuracy and speed (ANDRADE et al, 2017) compared to the tests accomplished at the hospitals in patients with clinical signs of sepsis (DELLINGER et al, 2013). Besides, it can avoid the empiric use of antibiotics, because reflects the most probable pathogens, contributing with the knowledge of the local epidemiology and the causes of the sepsis (ALVES, 2011) being possible to optimize the prophylactic treatment in newborn, reducing the death risk and expenses.…”

Section: Discussionmentioning

confidence: 99%

Neonatal sepsis: evaluation of risk factors and histopathological examination of placentas after delivery

Duarte¹,

Marcon²,

Akagi³

et al. 2019

Biosci. J.

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Neonatal sepsis is a clinical syndrome defined by systemic signs of infection in newborns accompanied by bacteremia. Can be responsible for serious consequences for the newborn child, characterized at the birth as early sepsis or late onset sepsis, with high rate of neonatal morbidity and mortality. Pathological agents such as Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Ureaplasma urealyticum and Mycoplasma hominis are most often responsible for intrauterine infections. The objective of this study is to evaluate the factors of neonatal sepsis predisposition in pregnant women through histopathological examination and the apoptotic index of placental tissues and detect DNA of E. coli and S. agalactiae using the Polymerase Chain Reaction (PCR). Histopathological analyses were made and the apoptotic index was determined to verify the levels of possible inflammatory infiltrates and cell death. Placenta samples were collected from November 2013 to May 2014. After DNA extraction, a PCR was performed amplifying the target fragment from the conserved regions of the rpoB (beta-RNA polymerase) polymorphism of E. coli and the factor 1 of S. agalactiae. The apoptosis index was tested with Acridine Orange and the histological procedure with Hematoxylin-Eosin staining. Among 100 samples of placental tissues analyzed by PCR, 48 represented the control group and did not present a risk factor associated with neonatal sepsis, and 52 samples representing the study group had at least one risk factor. Among these 52 samples, 7 (13.4%) had a PCR positive for E. coli. No placenta samples showed a positive PCR for S. agalactiae. The quantification of the apoptotic index did not show statistical significances between the groups and no inflammatory infiltrates were observed. However, histological sections showed fibrinoid necrosis, infarct areas and areas of calcification in all samples. Therefore, the results allow to conclude that the seven patients of experimental group with positive PCR for E. coli had eminent risk factors of neonatal sepsis, and the infection of the urinary tract (UTI) is the main aggravating circumstance. The histopathological examination demonstrated that the risk factors caused significant alterations, producing fibrinoid necrosis and infarcted areas in the placenta, contrary to apoptotic index that didn't differ from the group with unprecedented risk.

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Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction

Cited by 3 publications

References 30 publications

Streptococcus agalactiae in pregnant women in Brazil: prevalence, serotypes, and antibiotic resistance

Streptococcus agalactiae in pregnant women in Brazil: prevalence, serotypes, and antibiotic resistance

Distribution and Prevalence of Serotypes of Group B Streptococcus Isolated from Pregnant Women in 30 Countries: A Systematic Review

Neonatal sepsis: evaluation of risk factors and histopathological examination of placentas after delivery

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