Molecular Characterization of Giardia duodenalis and Cryptosporidium parvum in Fecal Samples of Individuals in Mongolia

Abstract

The Giardia and Cryptosporidium species are widespread and frequent diarrhea-related parasites affecting humans and other mammalian species. The prevalence of these parasites in Mongolia is currently unknown. Therefore, we performed molecular analyses of G. duodenalis and C. parvum in stool samples from 138 patients hospitalized with diarrhea in Mongolia using nested polymerase chain reaction (PCR). A total of 5 (3.62%) and 7 (5.07%) fecal samples were positive for G. duodenalis and C. parvum, respectively. Giardia duodenalis and C. parvum infections were prevalent in children < 9 years of age. The assemblage-specific fragment patterns for the β-giardin gene of G. duodenalis revealed that all five samples testing positive belonged to Assemblage A by the PCR-restriction fragment polymorphism method. For sequencing and phylogenetic analysis of the 18S rDNA and HSP70 genes of all seven patients testing positive the genes were further identified to be of the C. parvum bovine genotype. This study is the first to report the prevalence of G. duodenalis and C. parvum and its molecular characterization of fecal samples from individuals with diarrhea in Mongolia.

Giardia lamblia affecting humans in Sohag governorate and its relation with some physical measurements

“…[9], who stated that, the highest prevalence was between 1 -10 years. These results disagreed with Heehonget al [13], and El-Badryet al [14], who stated that giardiasis was highest in children aged 6 to 12 years. The results of this study showed that there was a decrease in weight, height, weight for height, and body mass index of children with Giardia infection by different proportions.…”

Giardia lamblia affecting humans in Sohag governorate and its related symptoms

“…These results agreed with Bernard et al [12], Who stated that the highest prevalence was between 3 to 5 years, and Mohran et al [9], who stated that, the highest prevalence was between 1 -10 years. These results disagreed with Heehong et al [13], and El-Badry et al [14], who stated that giardiasis was highest in children aged 6 to 12 years. As regards severity of diarrhea, in the present study, diarrhea was classified into mild, moderate, and severe.…”

Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA

“…All PCR assays were performed using 300 ng of purified DNA per reaction, PCR master mix (Qiagen, Germantown, MD, USA), parasite specific primers at 20 pmol/µL in a final volume of 25 µL. To detect G. lamblia , nested-PCR was performed using primers that target the β-giardin gene following a previously described protocol [ 12 ]. The forward primer for the first reaction was Gia7 (5′-AAGCCCGACGACCTCACCCGCAGTGC-3′) and the reverse primer was Gia759 (5′-GAGGCCGCCCTGGATCTTCGAGACGAC-3′).…”