Molecular characterization of Giardia duodenalis in children from the Cufada Lagoon Natural Park, Guinea-Bissau

Abstract

Molecular characterization of Giardia duodenalis in African countries is relatively scarce. The global understanding of Giardia epidemiology is reinforced when more data are available from highly endemic countries. In the present study, 50 fecal samples collected from children from Guinea-Bissau were screened for Giardia infection. Amplification of the Giardia ssu-rRNA fragment was achieved for 28 samples (28/50, 56.0 %) of which 23 (23/50, 46.0 %) positive samples for Giardia were detected through microscopy. Eighteen samples previous amplified for the ssu-rRNA locus were amplified for the bg gene fragment. Sequence analysis was performed in 26 and 17 samples for the ssu-rRNA and bg gene fragment, respectively. Our results revealed a predominance of assemblage B (22/26, 84.6 %), sequences with high genetic polymorphism among isolates belonging to this assemblage, making impossible the subassemblage determination. Assemblage A was identified in three isolates (3/26, 11.5 %), and our results strongly suggest that two isolates belong to subassemblage A2. This study provides information about G. duodenalis genotypes in a rural area of Guinea-Bissau and may contribute for a better understanding of giardiasis epidemiology in this country.

Genetic characterization of Giardia duodenalis by sequence analysis in humans and animals in Pemba Island, Tanzania

“…In no case were highlighted animal-specific assemblages (from C to G). The existing data on human genotypes characterized in African countries are still limited and fragmented, covering Egypt [15,16], Ethiopia [17], Western Sahara [18], Guinea Bissau [19] and Cote d'Ivoire [7]. The distribution of assemblages observed among our samples does not differ considerably from those observed in other African countries where a higher rate for assemblage B is already known, e.g.…”

Identical Assemblage of Giardia duodenalis in Humans, Animals and Vegetables in an Urban Area in Southern Brazil Indicates a Relationship among Them

“…Kohli et al (2008) [13], in North-eastern Brazil, reported that children infected with assemblage B eliminate larger numbers of cysts compared to those infected with assemblage A. A higher frequency of assemblage B has also been observed by other authors [29,33,34]. In contrast, Volotão et al (2007) [10], Souza et al (2007) [35] in Brazil and Zhang et al (2012) [36] in China found a predominance of genotype A, when specimens from humans and dogs were analysed.…”

Infection by Intestinal Parasites, Stunting and Anemia in School-Aged Children from Southern Angola

“…DNA was extracted from all samples in which Entamoeba histolytica/dispar cysts were detected by microscopy [ 12 – 13 ]. Molecular identification of E .…”