Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences

Amor, Nabil; Halajian, Ali; Farjallah, Sarra; Merella, Paolo; Saïd, Khaled; Slimane, Badreddine Ben

doi:10.1016/j.exppara.2011.03.011

Cited by 43 publications

(42 citation statements)

References 37 publications

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“…Several studies have shown that F. hepatica occurs in temperate areas, F. gigantica mainly in tropical zones, and both species overlap in subtropical areas Ashrafi et al 2006;Amor et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…But prior to the present study, there are no sufficient data to characterize Iranian F. hepatica existing genotypes based on variations in CO1 DNA sequences. Most of the previous markers for genetic characterization of Fasciola from Iran are limited to ITS1, ITS2, and 18S rDNA (Periago et al 2008;Ashrafi et al 2007;Karimi 2008;Rokni et al 2010;Amor et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

2011

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The present study compared the genetic variation among 19 different isolates of Fasciola hepatica from cattle and sheep in different areas of Iran using sequence data for mitochondrial DNA gene, the subunit 1 of cytochrome C oxidase gene (CO1). Four different CO1 genotypes were detected among F. hepatica isolates that showed five variable nucleotide positions (accession nos.; GQ398051, GQ398052, GQ398053, GQ398054). Nucleotide sequence variation among 19 isolates for CO1 analyzed in this study ranged from 0% to 0.98% in Iran. Among the five polymorphism sites identified in this study, only one (T to G at position 51 in 5'end of GQ175362) resulted in putative amino acid alteration of phenylalanine (TTT) to leucine (TTG) in CO1. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are likely not useful markers for Fasciola genotype classification. In addition, morphological analysis showed that the ratios of body length and body width of some (n = 5) of the 19 examined F. hepatica isolates were intermediate between F. hepatica and Fasciola gigantica, representing the substantial polymorphism of the F. hepatica species and the difficulty in the accurate recognition based on morphological features. In conclusion, Iranian F. hepatica exhibited the presence of considerable genetic diversity at CO1.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

2011

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“…In Iran, fasciolosis is present in many provinces including Kurdistan, Zanjan, Kermanshah, Mazandaran, Tehran, Azerbaijan, Gilan, Fars and Khuzestan [10] . Although the prevalence of fasciolosis among domestic animals is higher in the southern part of the country, the number of reported human disease cases is significantly higher in the Northern provinces situated along the shore of the Caspian Sea [11] .…”

Section: Introductionmentioning

confidence: 99%

“…Although the prevalence of fasciolosis among domestic animals is higher in the southern part of the country, the number of reported human disease cases is significantly higher in the Northern provinces situated along the shore of the Caspian Sea [11] . Both Fasciola hepatica and Fasciola gigantica are endemic in the Northern regions of Iran [10,11,12] . Dicrocoeliasis, a disease of grazing ruminants, is less severe than fasciolosis.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of fasciolosis and dicrocoeliosis in slaughtered sheep and goats in Amol Abattoir, Mazandaran, northern Iran

Khanjari

Bahonar

Fallah

et al. 2014

Asian Pacific Journal of Tropical Disease

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“…In recent years, an increasing number of human cases have been reported every year, particularly in the tropical developing countries, confirming its severe zoonotic transmission and emerging/ reemerging parasitic status (7,22,25,29,30,36,37). Both species coexist in some countries of North and East Africa such as Egypt, Ethiopia, Niger, Kenya, and Tanzania and in countries of Central/ Southeast Asia such as Pakistan, Iran, and China (6,8,9,25,30,38). Eating raw or improperly cooked vegetables/plants contaminated with Fasciola metacercariae can lead to fascioliasis, with potentially fatal injuries to the liver and biliary tract (30,48).…”

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confidence: 99%

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

Nguyen

et al. 2012

J Clin Microbiol

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A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of mitochondrial DNA (mtDNA) spanning the region of cox1-trnT-rrnL, two species-specific forward primers were designed, FHF (for F. hepatica) and FGF (for F. gigantica), and a single reverse primer, FHGR (common for both species). Conventional PCR followed by sequencing was applied using species-specific primer pairs to verify the specificity of primers and the identity of Fasciola DNA templates. Duplex PCR (using three primers) was used for testing with the DNA extracted from adult worms, miracidia, and eggs, producing amplicons of 1,031 bp for F. hepatica and 615 bp for F. gigantica. The duplex PCR failed to amplify from DNA of other common liver and intestinal trematodes, including two opisthorchiids, three heterophyids, an echinostomid, another fasciolid, and a taeniid cestode. The sensitivity assay showed that the duplex PCR limit of detection for each Fasciola species was between 0.012 ng and 0.006 ng DNA. Evaluation using DNA templates from 32 Fasciola samples (28 adults and 4 eggs) and from 25 field-collected stools of ruminants and humans revealed specific bands of the correct size and the presence of Fasciola species. This novel mtDNA duplex PCR is a sensitive and fast tool for accurate identification of Fasciola species in areas of distributional and zonal overlap.

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Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences

Cited by 43 publications

References 37 publications

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

Prevalence of fasciolosis and dicrocoeliosis in slaughtered sheep and goats in Amol Abattoir, Mazandaran, northern Iran

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

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