Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

Abstract

The present study compared the genetic variation among 19 different isolates of Fasciola hepatica from cattle and sheep in different areas of Iran using sequence data for mitochondrial DNA gene, the subunit 1 of cytochrome C oxidase gene (CO1). Four different CO1 genotypes were detected among F. hepatica isolates that showed five variable nucleotide positions (accession nos.; GQ398051, GQ398052, GQ398053, GQ398054). Nucleotide sequence variation among 19 isolates for CO1 analyzed in this study ranged from 0% to 0.98% in Iran. Among the five polymorphism sites identified in this study, only one (T to G at position 51 in 5'end of GQ175362) resulted in putative amino acid alteration of phenylalanine (TTT) to leucine (TTG) in CO1. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are likely not useful markers for Fasciola genotype classification. In addition, morphological analysis showed that the ratios of body length and body width of some (n = 5) of the 19 examined F. hepatica isolates were intermediate between F. hepatica and Fasciola gigantica, representing the substantial polymorphism of the F. hepatica species and the difficulty in the accurate recognition based on morphological features. In conclusion, Iranian F. hepatica exhibited the presence of considerable genetic diversity at CO1.

Transcriptomic Analysis of Ovaries from Pigs with High And Low Litter Size

“…The other most highly expressed genes may play other important roles in biological processes. CO1 and COX3 genes have been extensively utilized in polymorphic and phylogenetic analysis in some species [ 65 , 66 ] and GPX 3 is associated with certain cancers [ 67 , 68 ].…”

Genetic diversity of Fasciola spp. isolates from northern part of Iran: comparison with southwestern isolates

“…Human and animal fascioliasis is serious health and veterinary problems in Iran (Ashrafi et al 2015;Hosseini et al 2015;Moazeni et al 2012;Sarkari et al 2012;Mohammadi-Ghalehbin et al 2012). Northern Province of Gilan in Iran is one of the most important endemic regions of the disease where two massive outbreaks of human fascioliasis occurred in 1987 and in 1997 and affected more than 10,000 people (Ashrafi et al 2015;Rokni 2008).…”

Genetic diversity of Fasciola hepatica in Austria

“…Handling Editor: Una Ryan PCR For molecular analysis, 20-25 mg tissue were cut into small pieces with a scalpel. To avoid inclusion of foreign sperm, the tissue was taken from the apical zone of the flukes (Moazeni et al 2012). Whole-cell DNA was isolated using the QIAamp DNA Mini Kit (QIAGEN, Vienna).…”