Molecular characterization of exosomes and their microRNA cargo in human follicular fluid: bioinformatic analysis reveals that exosomal microRNAs control pathways involved in follicular maturation

Santonocito, Manuela; Vento, Marilena; Guglielmino, Maria Rosa; Battaglia, Rosalia; Wahlgren, Jessica; Ragusa, Marco; Barbagallo, Davide; Borzì, Placido; Rizzari, Simona; Maugeri, Marco; Scollo, Paolo; Tatone, Carla; Valadi, Hadi; Purrello, Michele; Pietro, Cinzia Di

doi:10.1016/j.fertnstert.2014.08.005

Cited by 194 publications

(172 citation statements)

References 55 publications

Supporting

Mentioning

168

Contrasting

Unclassified

Order By: Relevance

“…The presence of EVs containing microRNAs has been described in equine, bovine and human ovarian follicular fluid (da Silveira et al 2012, Sang et al 2013, Sohel et al 2013, Santonocito et al 2014, Navakanitworakul et al 2016. Using transmission electron microscopy and flow cytometry, da Silveira and coworkers reported the presence of a heterogenous sized population of EVs in equine follicular fluid (da Silveira et al 2012).…”

Section: Presence Of Evs Containing Micrornas In Follicular Fluidmentioning

confidence: 99%

“…Two separate studies reported that EV's containing microRNAs were also present in follicular fluid from women undergoing ICSI treatment (Sang et al 2013, Santonocito et al 2014. Isolated EVs contained 120 microRNAs according to TaqMan microRNA Array (Sang et al 2013).…”

Section: Presence Of Evs Containing Micrornas In Follicular Fluidmentioning

confidence: 99%

“…Although many of the EV-associated microRNAs were also detected in EV-free follicular fluid, 38 microRNAs were only detected in the isolated EV fraction. Furthermore, Santonocito and coworkers isolated EVs that were on average 40 nm in size from follicular fluid, and using a similar TaqMan PCR array, identified 22 microRNAs that were higher in EVs compared to plasma (Santonocito et al 2014). …”

Section: Presence Of Evs Containing Micrornas In Follicular Fluidmentioning

confidence: 99%

See 2 more Smart Citations

Cell-secreted vesicles containing microRNAs as regulators of gamete maturation

Silveira

Ávila

Garrett

et al. 2018

Journal of Endocrinology

View full text Add to dashboard Cite

Mammalian gamete maturation requires extensive signaling between germ cells and their surrounding somatic cells. In the ovary, theca cells, mural granulosa cells, cumulus cells and the oocyte all secrete factors throughout follicle growth and maturation that are critical for ovulation of a high-quality oocyte with the competence to develop into an embryo. Similarly, maturation of sperm occurs as it transits the epididymis during which epididymal epithelium and sperm exchange secretory factors that are required for sperm to gain motility and fertility. Recent studies in a variety of species have uncovered the presence of cell-secreted vesicles in follicular fluid (microvesicles and exosomes) and epididymal fluid (epididymosomes). Moreover, these cell-secreted vesicles contain small non-coding regulatory RNAs called microRNAs, which can be shuttled between maturing gametes and surrounding somatic cells. Although little is known about the exact mechanism of how microRNAs are loaded into these cell-secreted vesicles or are transferred and modulate gene expression and function in gametes, recent studies clearly suggest that cell-secreted vesicle microRNAs play a role in oocyte and sperm maturation. Moreover, a role for cell-secreted vesicular microRNAs in gamete maturation provides for novel opportunities to modulate and discover new diagnostic markers associated with male or female fertility. This manuscript provides an overview of cell-secreted vesicles in ovarian follicular fluid and epididymal fluid and microRNAs and discusses recent discoveries on the potential function of cell-secreted vesicles as carriers of microRNAs in oocyte and sperm maturation.

show abstract

Section: Presence Of Evs Containing Micrornas In Follicular Fluidmentioning

confidence: 99%

Section: Presence Of Evs Containing Micrornas In Follicular Fluidmentioning

confidence: 99%

Section: Presence Of Evs Containing Micrornas In Follicular Fluidmentioning

confidence: 99%

See 1 more Smart Citation

Cell-secreted vesicles containing microRNAs as regulators of gamete maturation

Silveira

Ávila

Garrett

et al. 2018

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Similarly, small RNA library constructed from cow ovary identified 50 known and 24 novel miRNAs, among which miRNAs; let-7a, let-7b, let-7c, miR-21, miR-23b, miR-24, miR-27a, miR-126 and miR-143 were found to be the most dominant ones (Hossain et al, 2009). The expression pattern of miRNAs in sheep ovaries during anestrus and the breeding season identified 97 known, 369 conserved and 17 predicted novel miRNAs (Di et al, 2014). Ovarian samples of uniparous and multiparous goats showed differential expression of miRNAs in which 8 miRNAs of the let-7 family; let-7b, let-7b-5p, let-7-5p, let-7c, let-7c-5p, let7f-5p, let-7f, let-7 and miR-140, miR-320a were the top 10 abundantly expressed miRNAs in both the uniparous and multiparous goat ovaries (Ling et al, 2014).…”

Section: Micrornas In Mammalian Ovarymentioning

confidence: 99%

“…While the majority of miRNAs are found intracellularly, tremendous numbers of miRNAs have been found to circulate in the extracellular space and biological fluids, including follicular fluid (Donadeu and Schauer, 2013; Sohel et al, 2013;Santonocito et al, 2014). Mammalian follicular fluid consists of a complex mixture of nucleic acids, proteins, metabolites and ions, which are known to be secreted by the oocyte, granulosa cells, theca cells and blood plasma components that come to the follicular fluid via theca capillaries (Revelli et al, 2009;Rodgers and IrvingRodgers, 2010).…”

Section: Extracellular Mirnas In Follicular Fluidmentioning

confidence: 99%

Dynamics and role of MicroRNAs during mammalian follicular development

Gebremedhn¹,

Pandey²,

Salilew‐Wondim³

et al. 2016

Anim Reprod

View full text Add to dashboard Cite

Ovarian functions, which involve dynamically regulated processes of selection, recruitment and dominance, are known to be regulated by an array of genes, which are expressed in spatiotemporal manner in follicular somatic cells and gametes. This differential expression of genes in mammalian follicular cells is partly regulated by posttranscriptional gene regulators named microRNAs (miRNAs). In addition to the cellular miRNAs, growing number of evidences are available on the potential role of extracellular vesicles mediated transfer of miRNAs in follicular fluid. These extracellular vesicles are shown to be involved in cellto-cell communication within the follicular environment. The molecular messages carried by the extracellular vesicles released into extracellular space are thought to be reflections of the physiological status of the cells from where they are released. Therefore due to their structural nature and potential to cargo several physiologically relevant molecules, exosomes have a great potential to be used as markers of oocyte developmental competence in follicular environment. Here, we review large sets of literatures to show the dynamic nature of miRNAs during various stages of mammalian follicular development, oocyte growth and the role of some of the miRNAs in ovarian cell functions. Moreover, the presence of microvesicle and exosome-coupled extracellular miRNAs in mammalian follicular fluid and their potential involvement in cellto-cell communication are briefly discussed.

show abstract

Exosomes as Theranostic Agents in Reproduction System

Izadpanah,

Yalameha,

Sani

et al. 2023

Advanced Biology

View full text Add to dashboard Cite

Exosomes (Exos), belonging to extracellular vesicles, are cell‐derived nano‐sized vesicles with the potential to carry different kinds of biological molecules. Many studies have proved the impacts of exosomal cargo on several biological processes in female and male reproductive systems. It is also hypothesized that changes in exosomal cargo are integral to the promotion of certain pathological conditions, thus Exos can be used as valid biomarkers for the diagnosis of infertility and other abnormal conditions. Here, efforts are made to collect some recent data related to the physiological significance of Exos in the reproductive system, and their potential therapeutic effects. It is anticipated that the current review article will lay the groundwork for elucidating the source and mechanisms by which Exos control the reproductive system additionally supplying fresh methods and concepts for the detection and treatment of disorders associated with fertility for future studies.

show abstract

Molecular characterization of exosomes and their microRNA cargo in human follicular fluid: bioinformatic analysis reveals that exosomal microRNAs control pathways involved in follicular maturation

Cited by 194 publications

References 55 publications

Cell-secreted vesicles containing microRNAs as regulators of gamete maturation

Cell-secreted vesicles containing microRNAs as regulators of gamete maturation

Dynamics and role of MicroRNAs during mammalian follicular development

Exosomes as Theranostic Agents in Reproduction System

Contact Info

Product

Resources

About