Molecular characterization of equine rotaviruses circulating in Argentinean foals during a 17-year surveillance period (1992–2008)

Garaicoechea, Lorena; Miño, S.; Ciarlet, Max; Férnandez, Francisco Tomás González; Barrandeguy, M.; Parreño, Viviana

doi:10.1016/j.vetmic.2010.08.032

Cited by 31 publications

(69 citation statements)

References 48 publications

(65 reference statements)

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“…9,14,17,28 while the other G genotypes (G5, G8, G10, G13) from foals were detected sporadically 4,13,[17][18][19] . While the P genotypes of rotavirus from human and other some animal species as bovine and porcine especially had been classified into 35 P types, equine rotaviruses are predominantly in P [12] genotype.…”

Section: Discussionmentioning

confidence: 99%

“…While the P genotypes of rotavirus from human and other some animal species as bovine and porcine especially had been classified into 35 P types, equine rotaviruses are predominantly in P [12] genotype. At this date, at least six different P types (P [12], P[3], P [11], P [1], P [18] and P [7]) of equine rotaviruses has been reported 4, 13,17,18,29 . The known binary combinations in group A equine rotaviruses are especially G14P [12] 14 and G3P [12] 14 , followed P [1] with combination G10 and G8, P [7] with combination G13 17,20 .…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, some inactivated vaccines for equine rotavirus had been developed and licenced USA and some European contries (the vaccine including H2 strain) and Japon (the vaccine including H0-5 strain), except Turkey 13,29,30 . However it is remained that the insufficient of the vaccine have been documented on several occations if foals are not supported by keeping the stable clean and by outsiding of their mares on the pastures.…”

Section: Discussionmentioning

confidence: 99%

“…The virus has two outer capsid proteins, VP7 and VP4, which are independently associated with the serotype specificities for G serotype (for glycoprotein) and the P serotype (for protease-sensitive protein), respectively 1 . In human and animal rotaviruses 27 G 13 and Japon [14][15][16] . Reports reveals that the majority of rotavirus strain from foals are either G3P [12] or G14P [12] 5,7,9, [11][12][13][14]16,17 .…”

Section: Makale Kodu (Article Code): Kvfd-2012-8186mentioning

confidence: 99%

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Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Alkan

Timurkan²,

Karayel³

2013

Kafkas Univ Vet Fak Derg

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SummaryIn this study, the aetiological agent of diarrhea in Arabian thoroughbred foals housed in a stud farms and it's molecular characterization were reported. Out of sampled seven foals with diarrhoea, five were detected positive by RT-PCR depending on the amplification of the VP6 gene of rotavirus. Then the aetiological agent was characterized genetically by sequence analysis of the genome segments encoding VP6, VP7, VP4 and NSP4 of rotavirus. Findings revealed that the Turkish equine rotavirus circulating within this stud farm belongs to G3 and P[12] genotype with E2 NSP4 and I6VP6. This is the first study to report the G and P genotypes of equine group A rotaviruses in Turkey. Keywords: Rotavirus, Equine, Genotyping, Diarrhoea, Turkey Türkiye'de Bir Arap Atı İşletmesindeki Taylarda Rotavirus İshali Salgını ÖzetBu çalışmada, bir at yetiştiriciliği işletmesinde bulunan safkan arap taylarındaki ishal olgusunun etiyolojik ajanının tespiti ve moleküler karakterizasyonu bildirildi. İshal semptomlu yedi taydan sağlanan materyallerin beşinde RT-PCR tekniği ile rotavirus VP6 geni yönünden pozitiflik saptandı. Daha sonra enfeksiyona neden olan virusun VP6, VP7, VP4 ve NSP4 gen bölgelerini kodlayan gen bölgelerinin dizi analizi yapıldı. Elde edilen verilere dayanılarak söz konusu at yetiştiriciliği işletmesinde enfeksiyona neden olan ERV saha suşunun E2 NSP4 ve I6VP6 ile ilişkili G3P[12] genotipinde olduğu belirlendi. Bu çalışma, Türkiye'de atlardaki grup A rotavirusların G ve P genotiplerinin bildirildiği ilk çalışmadır.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Makale Kodu (Article Code): Kvfd-2012-8186mentioning

confidence: 99%

See 2 more Smart Citations

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Alkan

Timurkan²,

Karayel³

2013

Kafkas Univ Vet Fak Derg

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“…Based on differences within RVA, a number of genotypes have been described for the VP4 (P[1]-P[37]), VP6 (I1-I16) and VP7 (G1-G27) gene (Estes & Greenberg, 2013;Matthijnssens et al, 2012;Trojnar et al, 2013 (Browning et al, 1991;Ciarlet et al, 2001;Isa et al, 1996;Miño et al, 2013;Taniguchi et al, 1994), G3P[12] and G14P[12] are the most prevalent strains associated with diarrhoea in foals worldwide. Rotavirus strains carrying the G3 genotype have been found in a wide range of host species (Estes & Greenberg, 2013;Martella et al, 2010), but the equine-like G3, G14 and P[12] genotypes have never been found producing outbreaks in any other host species than equines (Bailey et al, 2013;Garaicoechea et al, 2011;Matthijnssens et al, 2015;Weinberg et al, 2013). Genomes of equine RVA G3P [12] and G14P[12] are highly conserved, and only three gene segments have shown different genotypes (i.e.…”

Section: Introductionmentioning

confidence: 99%

Genetic linkage of capsid protein-encoding RNA segments in group A equine rotaviruses

Miño¹,

Barrandeguy

Parreño³

et al. 2016

Journal of General Virology

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Rotavirus virions are formed by three concentric protein layers that enclose the 11 dsRNA genome segments and the viral proteins VP1 and VP3. Interactions amongst the capsid proteins (VP2, VP6, VP7 and VP4) have been described to play a major role in viral fitness, whilst restricting the reassortment of the genomic segments during co-infection with different rotavirus strains. In this work we describe and characterize the linkage between VP6 and VP7 proteins based on structural and genomic analyses of group A rotavirus strains circulating in Argentinean horses. Strains with the VP7 genotype G3 showed a strong association with the VP6 genotype I6, whilst strains with G14 were associated with the I2 genotype. Most of the differences on the VP6 and VP7 proteins were observed in interactive regions between the two proteins, suggesting that VP6 : VP7 interactions may drive the co-evolution and co-segregation of their respective gene segments.

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Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010

et al. 2015

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Equine group A rotavirus (RVA) G3P[12] and G14P[12] strains cause gastroenteritis in foals worldwide. Both of these strains have been co-circulating in Japan since G14P[12] strains emerged in the late 1990s. Although it is important to comprehensively understand the evolution of RVA strains, whole-genome sequence data on recent equine RVA strains in Japan are lacking. Therefore, in this study, whole-genome analysis of 23 equine RVA isolates from the late 1990s and 2009-2010 and the vaccine strain RVA/Horse-tc/JPN/HO-5/1982/G3P[12] (HO-5) was performed. The G3 strains, including strain HO-5, shared a G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7 genotype constellation, and all of their 11 gene segments were highly conserved, regardless of the year of isolation. G14 strains also exhibited an identical genotype constellation (G14-P[12]-I2-R2-C2-M3-A10-N2-T3-E2-H7), but, phylogenetically, segregated into two lineages within the VP7-G14 and NSP4-E2 genotypes. G14 strains were closely related to G3 strains in their VP4, VP1-3, NSP1-3 and NSP5 gene segments. Interestingly, the NSP4 gene of all G3 and G14 strains isolated in the late 1990s branched into a bovine-RVA-like NSP4 gene cluster. These results indicate that, apart from VP7, VP6, and NSP4 genes, the Japanese equine RVA strains share a highly conserved genetic backbone, and that strains possessing a bovine-RVA-like NSP4 gene were predominant in the late 1990s in Japan.

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Molecular characterization of equine rotaviruses circulating in Argentinean foals during a 17-year surveillance period (1992–2008)

Cited by 31 publications

References 48 publications

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Genetic linkage of capsid protein-encoding RNA segments in group A equine rotaviruses

Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010

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