Molecular characterization of enterovirus 71 sibling strains for thermal adaption in Vero cells with adaptive laboratory evolution

Chen, Heng; Cheng, Yue; Liang, Xiangdang; Meng, Jia; Zuo, Haojiang; Su, Li-Yuan; Wang, Xixi; Yang, Cai-Bin; Luan, Rongsheng

doi:10.1016/j.meegid.2018.10.012

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…These features were proposed to help the virus survive in its host during the febrile stage and ensure successful neuroinvasion and replication in the CNS. Temperature-adapted strains were obtained in in vitro culture systems, supporting that the temperature-resistant phenotype could be acquired through mutation [40].…”

Section: Molecular Determinants That Influence Eva-71 Fitness and Virmentioning

confidence: 78%

Viral determinants that drive Enterovirus-A71 fitness and virulence

Ang

Chong

Alonso

2021

Emerging Microbes & Infections

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Hand, Foot and Mouth Disease (HFMD) is usually a self-limiting, mild childhood disease that is caused mainly by Coxsackie virus A16 (CVA16) and Enterovirus A71 (EV-A71), both members of the Picornaviridae family. However, recurring HFMD outbreaks and epidemics due to EV-A71 infection in the Western Pacific region, and the propensity of EV-A71 strains to cause severe neurological complications have made this neurotropic virus a serious public health concern in afflicted countries. High mutation rate leading to viral quasispecies combined with frequent intra-and inter-typic recombination events amongst co-circulating EV-A71 strains have contributed to the great diversity and fast evolution of EV-A71 genomes, making impossible any accurate prediction of the next epidemic strain. Comparative genome sequence analyses and mutagenesis approaches have led to the identification of a number of viral determinants involved in EV-A71 fitness and virulence. These viral determinants include amino acid residues located in the structural proteins of the virus, affecting attachment to the host cell surface, receptor binding, and uncoating events. Critical residues in non-structural proteins have also been identified, including 2C, 3A, 3C proteases and the RNA-dependent RNA polymerase. Finally, mutations altering key secondary structures in the 5' untranslated region were also found to influence EV-A71 fitness and virulence. While our current understanding of EV-A71 pathogenesis remains fragmented, these studies may help in the rational design of effective treatments and broadly protective vaccine candidates.

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Section: Molecular Determinants That Influence Eva-71 Fitness and Virmentioning

confidence: 78%

Viral determinants that drive Enterovirus-A71 fitness and virulence

Ang

Chong

Alonso

2021

Emerging Microbes & Infections

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Meteorological Factors and the Transmissibility of Hand, Foot, and Mouth Disease in Xiamen City, China

Yang

Zhao

et al. 2021

Front. Med.

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Background: As an emerging infectious disease, the prevention and control of hand, foot, and mouth disease (HFMD) poses a significant challenge to the development of public health in China. In this study, we aimed to explore the mechanism of the seasonal transmission characteristics of HFMD and to reveal the correlation and potential path between key meteorological factors and the transmissibility of HFMD.Methods: Combined with daily meteorological data such as average temperature, average relative humidity, average wind velocity, amount of precipitation, average air pressure, evaporation capacity, and sunshine duration, a database of HFMD incidence and meteorological factors was established. Spearman rank correlation was used to calculate the correlation between the various meteorological factors and the incidence of HFMD. The effective reproduction number (Reff) of HFMD was used as an intermediate variable to further quantify the dynamic relationship between the average temperature and Reff.Results: A total of 43,659 cases of HFMD were reported in Xiamen from 2014 to 2018. There was a significantly positive correlation between the average temperature and the incidence of HFMD (r = 0.596, p < 0.001), and a significantly negative correlation between the average air pressure and the incidence of HFMD (r = −0.511, p < 0.001). There was no correlation between the average wind velocity (r = 0.045, p > 0.05) or amount of precipitation (r = 0.043, p > 0.05) and incidence. There was a temperature threshold for HFMD's transmissibility. Owing to the seasonal transmission characteristics of HFMD in Xiamen, the temperature threshold of HFMD's transmissibility was 13.4–18.4°C and 14.5–29.3°C in spring and summer and in autumn and winter, respectively.Conclusions: HFMD's transmissibility may be affected by the average temperature; the temperature threshold range of transmissibility in autumn and winter is slightly wider than that in spring and summer. Based on our findings, we suggest that the relevant epidemic prevention departments should pay close attention to temperature changes in Xiamen to formulate timely prevention strategies before the arrival of the high-risk period.

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Molecular characterization of enterovirus 71 sibling strains for thermal adaption in Vero cells with adaptive laboratory evolution

Cited by 2 publications

References 27 publications

Viral determinants that drive Enterovirus-A71 fitness and virulence

Viral determinants that drive Enterovirus-A71 fitness and virulence

Meteorological Factors and the Transmissibility of Hand, Foot, and Mouth Disease in Xiamen City, China

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