Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

Wang, Yu-Chih; Chan, Jacky Peng Wen; Yeh, Kuang‐Sheng; Chang, Chi Chen; Hsuan, Shih-Ling; Hsieh, Yi Mei; Chang, Yu Han; Lai, Tan Chen; Lin, Wen-Hsin; Chen, Ter Hsin

doi:10.1016/j.vetmic.2009.09.067

Cited by 41 publications

(36 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All six isolates with an MIC ≥ 0.25 mg/L for enrofloxacin had an identical amino acid exchange (S83F) in the GyrA protein, which is in agreement with a previous study in A. pleuropneumoniae (Wang et al, 2010). Wang et al (2010) also reported that combinations of this and other mutations in gyrA, parC , and parE were associated with higher levels of resistance to enrofloxacin (MIC ≥ 1 mg/L), but these were not detected in our isolates.…”

Section: Discussionsupporting

confidence: 92%

“…Wang et al (2010) also reported that combinations of this and other mutations in gyrA, parC , and parE were associated with higher levels of resistance to enrofloxacin (MIC ≥ 1 mg/L), but these were not detected in our isolates. The six isolates with reduced susceptibility to enrofloxacin were isolated from samples submitted to the same APHA laboratory (Langford), and five were in the same year.…”

Section: Discussionmentioning

confidence: 49%

See 1 more Smart Citation

Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae

Bossé

Rogers

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data. Susceptibility of the isolates to nine antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, sulfisoxazole, tetracycline, tilmicosin, trimethoprim, and tylosin) was determined by agar dilution susceptibility test. Except for the macrolides tested, elevated MICs were highly correlated to the presence of AMR genes identified in wgs data using ResFinder or BLASTn. Of the isolates tested, 57% were resistant to tetracycline [MIC ≥ 4 mg/L; 94.8% with either tet(B) or tet(H)]; 48% to sulfisoxazole (MIC ≥ 256 mg/L or DD = 6; 100% with sul2), 20% to ampicillin (MIC ≥ 4 mg/L; 100% with blaROB-1), 17% to trimethoprim (MIC ≥ 32 mg/L; 100% with dfrA14), and 6% to enrofloxacin (MIC ≥ 0.25 mg/L; 100% with GyrAS83F). Only 33% of the isolates did not have detectable AMR genes, and were sensitive by MICs for the antimicrobial agents tested. Although 23 isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≤ 16 mg/L for both erythromycin and tilmicosin, and no macrolide resistance genes or known point mutations were detected. Other than the GyrAS83F mutation, the AMR genes detected were mapped to potential plasmids. In addition to presence on plasmid(s), the tet(B) gene was also found chromosomally either as part of a 56 kb integrative conjugative element (ICEApl1) in 21, or as part of a Tn7 insertion in 15 isolates. Our results indicate that, with the exception of macrolides, wgs data can be used to accurately predict resistance of A. pleuropneumoniae to the tested antimicrobial agents and provides added value for routine surveillance.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 49%

Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae

Bossé

Rogers

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In the body of different species of animals enrofloxacin is subjected to metabolic transformation into ciprofloxacin, which chemotherapeutic activity is comparable to the maternal substance (Riddle et al 2000, Grobbel et al 2007). The mechanism of action of enrofloxacin consists of inhibition of bacterial DNA gyrase, which plays basic role in the process of DNA replication, leading to inhibition of synthesis of bacterial proteins (Vancutsem et al 1990, Wang et al 2010. The metabolism takes place in the liver through the N-oxidation, N-dealkylation and deethylation processes.…”

Section: Introductionmentioning

confidence: 99%

Activity of superoxide dysmutase, catalase and glutathione peroxidase in rats exposed to chlorpyrifos and erofloxacin

Barski¹,

Spodniewska²,

Zasadowski³

2011

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of the study was to investigate the influence of administration of chlorpyrifos and/or enrofloxacin on the activity of chosen antioxidative enzymes i.e.: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes of rats. Chlorpyrifos was administered by stomach tube during 28 days at a dose of 3 mg/kg bw (0.02 LD 50 ), and enrofloxacin was administered by stomach tube at a dose of 5 mg/kg bw during 3 subsequent days. It was stated that administration of enrofloxacin at applied dose did not cause any major changes in the activity of investigated antioxidative enzymes. The four-week exposure of rats to chlorpyrifos caused noticeable decrease in SOD and CAT activity in erythrocytes of rats at the beginning of the experiment (up to 24 th hour) in comparison with the control group. The activity of GPx during all periods of the experiment was increased. In the group of animals in which both chlorpyrifos and enrofloxacin were applied, the profile of changes in activity of examined enzymes was similar to that one, which was observed after administration of chlorpyrifos exclusively, what may indicate lack of co-action between compounds used in the experiment.

show abstract

“…Cagliero et al (2007) selecionaram um mutante de C. jejuni multirresistente através do aumento das concentrações de enrofloxacina in vitro e afirmaram que a bomba de efluxo CmeABC tem relação com a resistência neste microrganismo. A resistência à ciprofloxacina conferida por bomba de efluxo também foi caracterizada em cepas de Actinobacillus pleuropneumoniae (Wang et al 2010).…”

Section: Discussionunclassified

Detecção de resistência às fluoroquinolonas em Campylobacter isolados de frangos de criação orgânica

et al. 2015

View full text Add to dashboard Cite

ABSTRACT.-Frasão B.S., Côrtes L.R., Nascimento E.R., Cunha N.C., Almeida V. Studies have shown that resistance to quinolones in Campylobacter strains is related with Threonine-86-Isoleucine mutation. In order to investigate the presence of this mutation in sensitive and resistant Campylobacter strains to ciprofloxacin and enrofloxacin, the cecal contents of 80 broilers from organic raising chickens, slaughtered under State Inspection Service (S.I.S) of the State of Rio de Janeiro, were collected and tested for the presence of Campylobacter. The determination of ciprofloxacin and enrofloxacin susceptibility was done by disk diffusion and agar dilution methods for determining the Minimum Inhibitory Concentration (MIC). The detection of mutation in Quinolone Resistance Determinant Region (QRDR) in gyrA gene was done by sequencing. Campylobacter was isolated from 100% of the samples, being 68.75% C. jejuni and 31.25% C. coli. By the disk diffusion method, resistance to ciprofloxacin was observed in all isolates and 56.25% of the strains were resistant to enrofloxacin. By agar dilution method, all strains were resistant to ciprofloxacin (MIC ≥ 16µg/mL to ≥ 64µg/mL) and full and intermediate resistance to enrofloxacin was detected in 42.50% (MIC ≥ 4-32μg/mL) and 38.75% (MIC =2μg/mL) of the strains, respectively. Mutation Thr-86-Ile was observed in 100% of the isolates investigated. In addition to this mutation, others no silent mutations and silent mutations (His-81-His, Ser-119-Ser, Ala-120-Ala, Phe-99-Phe, Ala-122-Ala, Gly-74-Gly, Ile-77-Ile, Ala-91-Ala, Leu-92-Leu, Val-93-Val, Ile-106-Ile, Thr-107-Thr, Gly-113-Gly, Ile-115-Ile, Gly-110-Gly) were detected. All the enrofloxacin--sensitive strains by the phenotypic methods had the Thr-86 to Ile substitution, which suggests other mechanisms contributing to enrofloxacin resistance in Campylobacter.

show abstract

Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

Cited by 41 publications

References 20 publications

Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae

Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae

Activity of superoxide dysmutase, catalase and glutathione peroxidase in rats exposed to chlorpyrifos and erofloxacin

Detecção de resistência às fluoroquinolonas em Campylobacter isolados de frangos de criação orgânica

Contact Info

Product

Resources

About