Molecular characterization of Ehrlichia canis from naturally infected dogs from the state of Rio de Janeiro

Costa, Renata Lins da; Paulino, Patrícia Gonzaga; Silva, Cláudia Bezerra da; Vitari, Gabriela Lopes Vivas; Peixoto, Maristela Peckle; Abreu, Ana Paula Martinez de; Santos, Huarrisson Azevedo; Massard, Carlos Luiz

doi:10.1007/s42770-018-0020-7

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…Cicuttin et al [11] performed molecular detection of E. canis on the canine blood samples from Buenos Aires, Argentina, and the PCR-positive rate was 6.7% (15/223) . da Costa et al [15] performed molecular detection of E. canis on Brazilian dogs, and the PCR-positive rate ranged from 14.6% to 42.3%. Daramola et al [12] detected blood samples from dogs in South West Nigeria used by nested PCR, and the positive rate was 22.9% (47/2015).…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

et al. 2020

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Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.

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Section: Discussionmentioning

confidence: 99%

“…the world existed great genetic variation and showed obvious genetic diversity [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

et al. 2020

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“…Na rotina veterinária, o diagnóstico da anaplasmose e erliquiose têm sido realizados através do histórico clínico, principalmente de cães infestados por carrapatos, associado aos achados hematológicos de testes parasitológicos, sorológicos e/ou moleculares ( COSTA et al, 2019;KOVACEVIC et al, 2018;PIRATAE et al, 2019;RUCKSAKEN et al 2019). E o tratamento terapêutico a base de antibioticoterapia associado a medicações de suporte (BERNARDES, 2022).…”

Section: Introductionunclassified

Alterações neurológicas em uma cadela com anaplasmose e erliquiose - relato de caso

Jesus¹,

Soares²,

Oliveira³

et al. 2023

Braz. J. Anim. Environ. Res.

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A erliquiose e anaplasmose são hemoparasitoses que vem acometendo cães e gatos atendidos em clínicas e hospitais veterinários do Brasil. Essas doenças parasitárias têm como principal vetor o carrapato-marrom (Rhipicephalus sanguineus), principal ectoparasito que infesta cães e gatos, podendo até parasitar acidentalmente os seres humanos. Esse ixodídeo ao realizar o repasto sanguíneo em seus hospedeiros pode inocular bactérias do gênero Ehrlichia sp. e Anaplasma sp., as quais irão se disseminar por células do sistema circulatório, podendo gerar diversas patologias. Devido à complexidade clínica de alguns casos, o objetivo desse trabalho é relatar as alterações neurológicas em uma cadela com anaplasmose e erliquiose. Foi atendido pela equipe do Laboratório de Doenças Parasitárias dos Animais da Universidade Federal de Sergipe, campus do Sertão, uma cadela, errante, de cinco anos de idade, pesando 17 kg, com historico de alterações neurológicas há cerca de 30 dias. Ao exame físico foi observado ataxia, disfunção neuromotora e vestibular, tremores, vocalização, desidratação, mucosas hipocoradas, hiperqueratose nos coxins, petéquias no abdômen, além da presença de carrapatos. Exames hematológicos revelaram anemia, trombocitopenia, além de mórulas de bactérias E. canis e A. platys. Não foram detectados anticorpos do tipo IgM para o vírus da cinomose canina. Como tratamento terapêutico foi utilizado antibioticoterapia associado a suplementação de vitaminas e controle dos ectoparasitos.

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“…to detect antibodies against the parasite after onset of the infection (Kottadamane et al 2017;Mitta et al 2017;Piratae et al 2019). Polymerase chain reaction (PCR) is a widely used molecular technique to confirm blood parasite infection due to its high sensitivity and specificity (Liu et al 2016;Csokai et al 2017;Yuasa et al 2017;Konto et al 2017;da Costa et al 2019); Kovacevic et al 2018;Rucksaken et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Clinical Observations, Hematological Profile, Serological Testing, and Molecular Detection of Ehrlichia canis in Veterinary Clinics in Bali, Indonesia

2022

Int J Vet Sci

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Ticks of Rhipicephalus species can serve as a vector for transmitting the Zoonotic Ehrlichiosis disease from dogs to humans. In Indonesia, epidemiological data on the prevalence of Ehrlichia canis are very limited. The incidence of Ehrlichiosis is usually reported based on clinical symptoms and serological results. In contrast, accurate diagnosis mainly relies on microscopic examination of a stained blood smear and polymerase chain reaction (PCR). The present study was conducted to evaluate the correlations among clinical observations, hematological profile, serological testing, and molecular detection of E. canis in dogs examined at different animal clinics in Bali, Indonesia. We collected 109 samples from Ehrlichiosis suspected dogs. The disease in these dogs was confirmed through a hematological profile, serologically, and PCR test. The hematological examination was performed with ICUBIO iCell-800Vet. We did a serological examination with Rapid Test BioNote© E. canis. DNA extraction was carried out with DNeasy Blood and Tissue Kit product Qiagen. PCR amplification was performed with Personal Thermal Cycler MJ Mini BIO-RAD. The results revealed that clinical signs such as epistaxis, fever, and pale mucous membranes were strongly associated with serological detection of E. canis. In contrast, none of these signs was significantly correlated with PCR detection of E. canis. Total erythrocytes counts were significantly associated with serologic detection. The total erythrocytes, thrombocytes, and hemoglobin levels were significantly associated with PCR detection. The 16S-rDNA, collected from PCR and amplified, showed the E. canis gene, indicating that E. canis organisms were found in Bali. E canis Bali is one cluster with clusters from South America, Europe, and Asian countries like Thailand, Taiwan, Japan, and Israel. In conclusion, E. canis infection was confirmed through clinical, serological, and molecular approaches in dogs in Bali, Indonesia.

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Molecular characterization of Ehrlichia canis from naturally infected dogs from the state of Rio de Janeiro

Cited by 10 publications

References 34 publications

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

Alterações neurológicas em uma cadela com anaplasmose e erliquiose - relato de caso

Clinical Observations, Hematological Profile, Serological Testing, and Molecular Detection of Ehrlichia canis in Veterinary Clinics in Bali, Indonesia

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