Molecular characterization of dengue and chikungunya virus strains circulating in New Delhi, India

Afreen, Nazia; Deeba, Farah; Khan, Wajihul Hasan; Haider, Shakir Hussain; Kazim, Syed Naqui; Ishrat, Romana; Naqvi, Irshad H.; Shareef, M.; Broor, Shobha; Ahmed, Anwar; Parveen, Shama

doi:10.1111/1348-0421.12209

Cited by 47 publications

(51 citation statements)

References 41 publications

(50 reference statements)

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“…Similarly 43% co-infection was reported from Davangere, Karnataka [5] and 57% from Thiruvananthapuram, Kerala [6], 32·5% from Moreh, Manipur [38] and 19% from Delhi [3]. Our earlier work reported 9% co-infections during 2011 [7], 14% during 2013 [2] and 18% during 4 years of study from 2011-14 [39] in Delhi. Dual infection was also reported in other investigations from Delhi [4,40,41].…”

Section: Discussionsupporting

confidence: 63%

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Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

et al. 2016

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SUMMARYDengue fever is an arthropod-borne viral infection that has become endemic in several parts of India including Delhi. We studied occurrence of co-infection with dengue viruses during an outbreak in New Delhi, India in 2014. For the present study, blood samples collected from symptomatic patients were analysed by RT-PCR. Eighty percent of the samples were positive for dengue virus. The result showed that DENV-1 (77%) was the predominant serotype followed by DENV-2 (60%). Concurrent infection with more than one serotype was identified in 43% of the positive samples. Phylogenetic analysis clustered DENV-1 strains with the American African and DENV-2 strains in Cosmopolitan genotypes. Four common amino-acid mutations were identified in the envelope gene of DENV-1 sequences (F337I, A369T, V380I and L402F) and one common mutation (N390S) in the DENV-2 sequences. Further analysis revealed purifying selection in both the serotypes. A significant number of patients were co-infected with DENV-1 and DENV-2 serotypes. Although we do not have direct evidence to demonstrate co-evolution of these two stereotypes, nonetheless their simultaneous occurrence does indicate that they are favoured by evolutionary forces. An ongoing surveillance and careful analysis of future outbreaks will strengthen the concept of co-evolution or otherwise. Whether the concurrent dengue viral infection is correlated with disease severity in a given population is another aspect to be pursued. This study is envisaged to be useful for future reference in the context of overall epidemiology.

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Section: Discussionsupporting

confidence: 63%

“…Envelope protein gene regions of two different lengths, 448 bp and 574 bp, were amplified for DENV-1 and DENV-2, respectively. These two PCR reactions were performed as described previously [7]. The required bands were excised from agarose gel.…”

Section: Sequencing Pcrmentioning

confidence: 99%

Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

et al. 2016

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“…The Chikungunya viral infection share common symptoms with the dengue viral infection characterized by sudden onset of fever, vomiting, nausea, rashes, arthralgia and myalgia [2,13]. Both CHIKV and DENV are spread by Aedes aegypti and Aedes albopictus mosquitoes [3].…”

Section: Introductionmentioning

confidence: 99%

Potential entry inhibitors of the envelope protein (E2) of Chikungunya virus: in silico structural modeling, docking and molecular dynamic studies

et al. 2017

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Chikungunya fever is an arboviral infection caused by the Chikungunya virus (CHIKV) and is transmitted by Aedes mosquito. The envelope protein (E2) of Chikungunya virus is involved in attachment of virion with the host cell. The present study was conceptualized to determine the structure of E2 protein of CHIKV and to identify the potential viral entry inhibitors. The secondary and tertiary structure of E2 protein was determined using bioinformatics tools. The mutational analysis of the E2 protein suggested that mutations may stabilize or de-stabilize the structure which may affect the structure-function relationship. In silico screening of various compounds from different databases identified two lead molecules i.e. phenothiazine and bafilomycin. Molecular docking and MD simulation studies of the E2 protein and compound complexes was carried out. This analysis revealed that bafilomycin has high docking score and thus high binding affinity with E2 protein suggesting stable protein-ligand interaction. Further, MD simulations suggested that both the compounds were stabilizing E2 protein. Thus, bafilomycin and phenothiazine may be considered as the lead compounds in terms of potential entry inhibitor for CHIKV. Further, these results should be confirmed by comprehensive cell culture, cytotoxic assays and animal experiments. Certain derivatives of phenothiazines can also be explored in future studies for entry inhibitors against CHIKV. The present investigation thus provides insight into protein structural dynamics of the envelope protein of CHIKV. In addition the study also provides information on the dynamics of interaction of E2 protein with entry inhibitors that will contribute towards structure based drug design.

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“…CHIKV and DENV nucleic acids were detected from clinical samples obtained during dengue fever outbreak of 2006 in Delhi [5]. Subsequently, co-infection of DENV and CHIKV were again reported from Delhi in 2014 and from Odisha and Maharashtra in 2015 [1,13]. The present study was undertaken to examine CHIKV infection in patients with suspected dengue fever during dengue outbreak in 2010 in Delhi.…”

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confidence: 95%

Chikungunya: a reemerging infection spreading during 2010 dengue fever outbreak in National Capital Region of India

et al. 2016

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Chikungunya fever is an important reemerging arbovirus illness, which is transmitted by the same vector as of dengue virus. Many cases of concurrent infections with multiple dengue virus serotypes have been reported in many countries. Also, concurrent infection with Chikungunya virus and dengue virus has been reported in the past in Delhi. Therefore, this study was done to detect Chikungunya IgM antibodies in suspected dengue fever patients. In this study, 1666 serum samples suspected of dengue fever and collected during the outbreak period (August 2010-December 2010) were tested for dengue IgM antibodies, of which 736 tested negative. Of the 736 dengue IgM negative sera, 666 were tested for Chikungunya IgM antibodies. The demographic profile and essential laboratory investigations were recorded. Chikungunya IgM was detected in 9.91 % of the patients. During the post-monsoon period though dengue dominated in numbers, the number of Chikungunya fever cases increased gradually followed by an abrupt decrease with the onset of winter. The Chikungunya IgM positive patients were suffering from fever of more than 5 days duration and had thrombocytopenia. Due to similarity in clinical features and vector transmitting dengue and Chikungunya virus, continuous surveillance of both dengue fever and Chikungunya fever is desirable for better management and epidemiological assessment.

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Molecular characterization of dengue and chikungunya virus strains circulating in New Delhi, India

Cited by 47 publications

References 41 publications

Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

Potential entry inhibitors of the envelope protein (E2) of Chikungunya virus: in silico structural modeling, docking and molecular dynamic studies

Chikungunya: a reemerging infection spreading during 2010 dengue fever outbreak in National Capital Region of India

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