Molecular characterization of coxsackievirus B5 isolates

Rezig, Dorra; Yahia, Ahlem Ben; Abdallah, H.; Bahri, Olfa; Triki, H.

doi:10.1002/jmv.10579

Cited by 27 publications

(21 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have provided some insight into the genetic diversity within the CVB5 serotype (50,80,93). In the present study, an ML approach was applied to extend previous studies and to analyze the global genetic diversity among contemporary CVB5 isolates collected over a 25-year period.…”

Section: Discussionmentioning

confidence: 97%

Characterization of a Putative Ancestor of Coxsackievirus B5

Gullberg

Tolf

Jönsson

et al. 2010

J Virol

View full text Add to dashboard Cite

Like other RNA viruses, coxsackievirus B5 (CVB5) exists as circulating heterogeneous populations of genetic variants. In this study, we present the reconstruction and characterization of a probable ancestral virion of CVB5. Phylogenetic analyses based on capsid protein-encoding regions (the VP1 gene of 41 clinical isolates and the entire P1 region of eight clinical isolates) of CVB5 revealed two major cocirculating lineages. Ancestral capsid sequences were inferred from sequences of these contemporary CVB5 isolates by using maximum likelihood methods. By using Bayesian phylodynamic analysis, the inferred VP1 ancestral sequence dated back to 1854 (1807 to 1898). In order to study the properties of the putative ancestral capsid, the entire ancestral P1 sequence was synthesized de novo and inserted into the replicative backbone of an infectious CVB5 cDNA clone. Characterization of the recombinant virus in cell culture showed that fully functional infectious virus particles were assembled and that these viruses displayed properties similar to those of modern isolates in terms of receptor preferences, plaque phenotypes, growth characteristics, and cell tropism. This is the first report describing the resurrection and characterization of a picornavirus with a putative ancestral capsid. Our approach, including a phylogenetics-based reconstruction of viral predecessors, could serve as a starting point for experimental studies of viral evolution and might also provide an alternative strategy for the development of vaccines.

show abstract

Section: Discussionmentioning

confidence: 97%

Characterization of a Putative Ancestor of Coxsackievirus B5

Gullberg

Tolf

Jönsson

et al. 2010

J Virol

View full text Add to dashboard Cite

show abstract

“…Polioviruses were identified by the presence of a cytopathic effect (CPE) on L20B cell lines; they were then serotyped and intratyped by real-time PCR (16). Nonpolio enteroviruses were identified by the presence of a CPE on RD and/or Hep2-C cells and typed by partial sequencing in the VP1 gene as described previously (25).…”

Section: Methodsmentioning

confidence: 99%

High Susceptibility for Enterovirus Infection and Virus Excretion Features in Tunisian Patients with Primary Immunodeficiencies

Driss

Ben‐Mustapha

Mellouli

et al. 2012

Clin. Vaccine Immunol.

View full text Add to dashboard Cite

To estimate the susceptibility to enterovirus infection and the frequency of long-term poliovirus excreters in Tunisian patients with primary immunodeficiencies (PIDs), enteroviruses were assessed in stool specimens of 82 patients with humoral, combined, and other PIDs. Isolated viruses were typed and intratyped by standard molecular techniques, and the whole VP1 region of poliovirus isolates was sequenced. Polioviruses were detected in 6 patients; all isolates were vaccine related. Five patients rapidly stopped excretion; one excreted a poliovirus type 1 isolate for several months, and the isolate accumulated up to 14 mutations in the VP1 region. Nonpolio enteroviruses were identified in 6 patients; 4 of them kept excreting the same strain for more than 6 months. The rate of enterovirus infection was 13.4% of the PID patients and 20.7% of those with an IgG defect; it greatly exceeded the rates generally found in Tunisian supposed-immunocompetent individuals (4.1% during the study period; P ‫؍‬ 0.001 and P < 0.0001, respectively). Interestingly, patients with combined immunodeficiencies were at a higher risk for enterovirus infection than those with an exclusively B cell defect. A major histocompatibility complex (MHC) class II antigen expression defect was found in 54% of enterovirus-positive patients and in the unique long-term poliovirus excreter. The study results also suggest that substitutive immunoglobulin therapy may help clearance of a poliovirus infection and that most PID patients have the ability to stop poliovirus excretion within a limited period. However, the high susceptibility of these patients to enterovirus infection reinforces the need for enhanced surveillance of these patients until the use of oral poliovirus vaccine (OPV) is stopped.

show abstract

“…NPEV can also be responsible for acute flaccid paralysis, a syndrome that mimics poliomyelitis. In Europe, 84 NPEV associated cases were registered in 2005 by the Polio Lab Network (3).Enteroviruses have a positive single-stranded RNA genome subjected to high mutation rates and frequent genetic recombination events (1, 17, 30-33, 37, 46-48, 58, 60, 61) and thereby display a great diversity (10,41,52,55,59). More than 90 serotypes have been characterized among the human enteroviruses (HEVs), but only 68 are recognized in the current taxonomy (62).…”

mentioning

confidence: 99%

Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid

Mirand¹,

Henquell

Archimbaud³

et al. 2008

J Clin Microbiol

View full text Add to dashboard Cite

Enterovirus infections were investigated with special emphasis on performing rapid molecular identification of enterovirus serotypes responsible for aseptic meningitis directly in cerebrospinal fluid (CSF). Enterovirus genotyping was carried out directly with specimens tested for the diagnostic procedure, using two seminested PCR assays designed to amplify the complete and partial gene sequences encoding the VP1 and VP4/VP2 capsid proteins, respectively. The method was used for identifying the enterovirus serotypes involved in meningitis in 45 patients admitted in 2005. Enterovirus genotyping was achieved in 98% of the patients studied, and we obtained evidence of 10 of the most frequent serotypes identified earlier by genotyping of virus isolates. The method was applied for the prospective investigation of 54 patients with meningitis admitted consecutively in 2006. The enterovirus serotypes involved were identified with the cerebrospinal fluid (CSF) of 52 patients (96%) and comprised 13 serotypes within the human enterovirus B species and 1 within the human enterovirus A species. The three most common serotypes were echovirus 13 (E13; 24%), E6 (23%), and coxsackievirus B5 (11.5%), a pattern different from that observed in 2005. Genotyping of virus isolates was also performed in 35 patients in 2006 (meningitis, n ‫؍‬ 31; other diseases, n ‫؍‬ 4). By comparison, direct genotyping in CSF yielded a more complete pattern of enterovirus serotypes, thereby allowing the detection of rare serotypes: three less common serotypes (CB2, E21, and E27) were not detected by indirect genotyping alone. The study shows the feasibility of prospective enterovirus genotyping within 1 week in a laboratory setting.Enterovirus infections comprise a wide spectrum of clinical presentations and diseases (35). As with poliomyelitis, the most problematic clinical syndromes caused by nonpolio enteroviruses (NPEV)-meningitis, encephalitis, and acute flaccid paralysis-result from infection of the central nervous system (CNS) and are major causes of illness and morbidity in both children and adults (57).Enterovirus meningitis (also known as aseptic meningitis) is the most commonly observed CNS infection, and its clinical presentation varies with the patient's age and immune status (38,39). It occurs typically during outbreaks (in restricted geographical areas or communities) in the summer and autumn, leading to increased admissions to hospital wards for short periods. Unlike meningitis, enterovirus encephalitis is a more severe acute illness with more long-term sequelae (16) and, while it is less frequently observed than meningitis, its frequency should not be underestimated. For instance, in the California Encephalitis Project, an enterovirus infection was detected in 25% of patients with a viral etiology; 24% had a herpes simplex virus type 1 infection (20). NPEV can also be responsible for acute flaccid paralysis, a syndrome that mimics poliomyelitis. In Europe, 84 NPEV associated cases were registered in 2005 by the Polio Lab Network (3).En...

show abstract

Molecular characterization of coxsackievirus B5 isolates

Cited by 27 publications

References 37 publications

Characterization of a Putative Ancestor of Coxsackievirus B5

Characterization of a Putative Ancestor of Coxsackievirus B5

High Susceptibility for Enterovirus Infection and Virus Excretion Features in Tunisian Patients with Primary Immunodeficiencies

Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid

Contact Info

Product

Resources

About