Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR

Guimarães, Alexandre Silva; Dorneles, Elaine Maria Seles; Andrade, Gláucia Manzan Queiroz de; Lage, Andrey Pereira; Miyoshi, Anderson; Azevedo, Vasco; Gouveia, Aurora Maria Guimarães; Heinemann, Marcos Bryan

doi:10.1016/j.vetmic.2011.06.002

Cited by 30 publications

(46 citation statements)

References 21 publications

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“…The new genotypes constituted 37.14% of all typed C. pseudotuberculosis goat isolates. In the ERIC-2-PCR assay, only 2 genotypic profiles matched previously reported genotypes (Guimarães et al, 2011b), whereas 85.71% of the studied goat isolates had not been previously described. Fingerprinting of C. pseudotuberculosis isolates from goats using ERIC-1+2-PCR also revealed 3 genotypes that were identical to genotypes previously described in C. pseudotuberculosis isolates from sheep (Guimarães et al, 2011b), whereas 45.71% of the strains represented new genotypes.…”

Section: Resultsmentioning

confidence: 56%

“…In the ERIC-2-PCR assay, only 2 genotypic profiles matched previously reported genotypes (Guimarães et al, 2011b), whereas 85.71% of the studied goat isolates had not been previously described. Fingerprinting of C. pseudotuberculosis isolates from goats using ERIC-1+2-PCR also revealed 3 genotypes that were identical to genotypes previously described in C. pseudotuberculosis isolates from sheep (Guimarães et al, 2011b), whereas 45.71% of the strains represented new genotypes. For all ERIC-PCR assays, the previously described genotypes were assigned the same identification label used by Guimarães et al (2011b), and the new genotypes were labeled sequentially in the same way (see Figures 1, 2, and 3).…”

Section: Resultsmentioning

confidence: 56%

“…Of the 8 genotypes identified with ERIC-1-PCR among the C. pseudotuberculosis isolates from goats, 3 had been previously described in sheep in Minas Gerais State, Brazil (Guimarães et al, 2011b). The new genotypes constituted 37.14% of all typed C. pseudotuberculosis goat isolates.…”

Section: Resultsmentioning

confidence: 90%

“…pseudotuberculosis isolates were fingerprinted with RAPD using the following primers (Versalovic et al, 1991) as described by Guimarães et al (2011b): ERIC-1R (5ꞌ-ATGTAAGCTCCTGGGGATTCAC-3ꞌ), ERIC-2 (5ꞌ-AAGTAAGTGACTGGGGTGAGC G-3ꞌ), and the ERIC-1R+ERIC-2 primer pair (Invitrogen, USA). To avoid misinterpretation owing to interassay variability, all strains were assayed at the same time and under similar conditions.…”

Section: Eric-pcr Conditionsmentioning

confidence: 99%

“…Subtyping of C. pseudotuberculosis strains isolated from various hosts using existing genotyping methods has proven to be difficult. Recently, we proposed a typing method based on enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), which we found to be an excellent test for the genetic discrimination of C. pseudotuberculosis strains from sheep and had high resolution and good repeatability (Guimarães et al, 2011b). Thus, the aims of this study were to evaluate the genetic diversity of C. pseudotuberculosis isolates in the Sertão Region of Pernambuco State, Brazil, using ERIC-PCR and to investigate the potential use of this technique as a tool for molecular typing of C. pseudotuberculosis strains isolated from goats.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Short Communication Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

Dorneles

Santana²,

Andrade³

et al. 2012

Genet. Mol. Res.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 90%

Section: Eric-pcr Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Short Communication Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

Dorneles

Santana²,

Andrade³

et al. 2012

Genet. Mol. Res.

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Molecular Biology Techniques for the Identification and Genotyping of Microorganisms

Gohil

Panchasara

Patel

et al. 2019

Microbial Genomics in Sustainable Agroecosystems

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Silver nanoparticle effect on Salmonella enterica isolated from Northern West Egypt food, poultry, and calves

Torky

Khaliel

Sedeek

et al. 2022

Appl Microbiol Biotechnol

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A total no. of 65 Salmonella enterica isolates recovered from food samples, feces of diarrheic calves, poultry, and hospital patient in large five cities at Northern West Egypt were obtained from the Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt. The 65 Salmonella enterica isolates had the invA gene were grouped into 11 Salmonella enterica serovars with dominance of S. Enteritidis and S. Kentucky serovars. Their resistance pattern were characterized by using 18 antibiotics from different classes. Approximately 80% of the isolates were multidrug resistant (MDR). Enterobacterial repetitive intergenic consequences polymerase chain reaction (ERIC-PCR) typing of 7 strains of S. Enteritidis showed 5 clusters with dissimilarity 25%. S. Enteritidis clusters in 2 main groups A and B. Group A have 2 human strain (HE2 and HE3) and one food origin (FE7) with a similarity 99%. Group B divided into B1 (FE2) and B2 (FE3) with a similarity ratio ≥ 93%, while ERIC-PCR analysis of 5 strains of S. Kentucky revealed 4 ERIC types, clustered in 2 main groups A and B with similarity 75%. We studied the effect of silver nanoparticles (Ag-NPs) on 10 antibiotic resistant strains of S. Enteritidis and S. Kentucky. The broth microdilution minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were detected. Evaluation of the affection using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed different ratios of Ag-NPs and microorganism as well as at different contact time ended finally with morphological alteration of the bacteria. We submitted new method in vivo to explore the activity of nanosilver in chicken. Key points • Importance of ERIC-PCR to determine the relatedness between Salmonella isolates. • Effect of silver nanoparticles to confront the antibacterial resistance. • Studying the effect of silver nanoparticles in vivo on infected chicken with Salmonella.

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Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR

Cited by 30 publications

References 21 publications

Short Communication Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

Short Communication Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR

Molecular Biology Techniques for the Identification and Genotyping of Microorganisms

Silver nanoparticle effect on Salmonella enterica isolated from Northern West Egypt food, poultry, and calves

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