Molecular Characterization of chickpea genotypes and Identification of true hybrids by molecular markers

Abstract

Chickpea (Cicer arietinum L.) is the third most important grain legume cultivated in the arid and semi-arid regions of the world. In the present study Six crossing combinations were executed in chickpea comprising Chanoli and PKV Kabuli 4 as female parents and Virat, BDNGK-798 and WR- 315 as resistant male parents. Total 54 markers including 13 SCoT, 31 SSR, 5 STMS, 3 RAPD, 1 SCAR, and 1 ISSR, used for parental polymorphism and polymorphic markers UBC-855, 66 % for TA-59 and 100 % for TA-110, TA-135 and GA-16 were further used to hybridity assessments of F1 plants. The PIC value for polymorphic markers ranged from 0.15 to 0.89 with an average value of 0.46. The highest PIC value was observed in UBC-855 marker (0.89), followed by TA-135 (0.62), TA-59 (0.50), and GA-16 (0.16) and lowest PIC value observed in TA-110 (0.15). From total crosses 31 F1 plants of six crosses were screened for true F1 hybridity assessment. STMS marker TA-59 was used for F1 hybrid purity assessment. This marker screened 31 F1 plants. TA-59 shows specific size amplicon in female and male parents. The results of this investigation proved that SSR markers are well polymorphic and more useful markers within species of chickpea genotypes to perform the molecular characterization and to test the genetic hybridity of F1 plants. Among the tested SSR markers TA-59, TA-110, TA-135, GA-16, UBC-855 shows high percentage of polymorphism and PIC value which will were more helpful for parental diversity analysis and hybridity assessment.