Aim:The main aim of the present study was to develop nanotechnology-based solutions for the management of a fungus, Phytophthora parasitica causing gummosis in citrus.
Methods and Results: Biogenic copper nanoparticles (CuONPs) were synthesized using two different biocontrol agents, Pseudomonas fluorescens and Trichoderma viride and characterized using different analytical techniques. Furthermore, in vitro (at the concentrations of 10, 15, 30, 50, 70, 100 and 150 mg/L) and in vivo (at the concentration of 100 mg/L) activities of these nanoparticles were evaluated for their antifungal efficacy against P. parasitica. The results obtained confirmed the synthesis of irregular-shaped CuONPs having a size in the range 40-100 nm in case of P. fluorescens, whereas, spherical CuONPs in the size range 20-80 were recorded in case of T.viride. As far as the in vitro antifungal efficacies of both these CuONPs is concerned, the maximum percent growth inhibition was observed in case of CuONPs synthesized from T. viride compared to CuONPs from P. fluorescens. However, in case of in vivo antifungal efficacies, CuONPs synthesized from T. viride showed the activity significantly higher than the conventionally used Bordeaux mixture.
Conclusions:It can be concluded that biosynthesized CuONPs can be effectively used as a potential fungicide against P. parasitica.
Significance and Impact of the Study:The application of nanoparticles having antifungal activities can be used as alternative fungicides to the conventional chemical fungicides. It has the potential to revolutionize the existing management strategies available for plant pathogenic fungi.
Foxtail millet (Setaria italica L.) is nutri-cereal crop having it is rich in β-carotene, vitamin B-complex and micronutrients like minerals. In the present research, we have studied biochemical properties and molecular profiling to identify the core set of foxtail millet (Setaria italic L.) accessions for high Iron (Fe) and Zinc (Zn) content. Total seventy-nine accessions and selected mutants variety PS4 of foxtail millet were used. The biochemical investigation revealed that accessions M2-106, IC120407, M3-61/HB-13, and IC120255 consist of high iron and zinc content. The genetic variability among the genotypes was revealed by 28 Promoter Anchored Amplified Polymorphic (PAAP-RAPD) primers of which OPE9+GC1, OPE9+CA1, UBC001+CA1, UBC001+TA1, UBC693+G1 showed 100% polymorphism, whereas UBC693+GC1 and OPE7+G1 showed 88% and 80% polymorphism, respectively, with an average of 45.95 % polymorphism. Total alleles per locus were 3.31, whereas, the average number of monomorphic and polymorphic alleles were 1.72 and 1.56, respectively. The extent of polymorphic information content (PIC) of PAAP-RAPD loci ranged from 0.5 to 0.87 with an average value of 0.41. For PAAP-RAPD, the maximum PIC value was observed in marker UBC693+GC1 (0.87 %) and the minimum were OPE5+CA1 and OPE9+CA1 (0.5 %). Molecular characterizations result showing highest similarity (0.932) between accessions Shrilakshmi and Prasad, whereas, the lowest similarity coefficient was observed between IC120255 and M3-75/AM-1 (0.697) with PS4.UPGMA dendrogram grouped the foxtail millet accessions in five clusters which marked high diversity in M3-61/HB-13 and M3-75/AM-1. It implies that PAAP-RAPD markers are significantly screened in the foxtail millet accessions and have enumerated high genetic diversity.
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