Molecular characterization of canine parvovirus strains in Argentina: Detection of the pathogenic variant CPV2c in vaccinated dogs

Calderón, Marina Gallo; Mattion, Nora; Bucafusco, Danilo; Fogel, Fernando; Remorini, Patricia; Torre, J.L. La

doi:10.1016/j.jviromet.2009.03.013

Cited by 61 publications

(69 citation statements)

References 40 publications

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“…It was previously reported that this is the predominant variant in Uruguay (PEREZ et al, 2007), Argentina (CALDERON et al, 2009) andBrazil (STRECK et al, 2009;CASTRO et al, 2010). To our knowledge the isolation in cell culture of CPV-2c strain was not described in the neighboiring countries, therefore this research is the first report in Uruguay and in the region about CPV-2c isolation in A-72 canine cell line.…”

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 72%

“…This is a mutant of CPV-2b which involved the substitution Aspartate (Asp) 426 Glutamate (Glu) occurring in a main neutralizing epitope of the capsid (BUONAVOGLIA et al, 2001). Since its has been identified, CPV-2c has been spread over local canine population and has also been found in other European countries (DECARO et al, 2007), in Asia (NAKAMURA et al, 2004;NANDI et al, 2009), in Africa (TOUIHRI et al, 2009) and in the America; USA (HONG et al, 2007;KAPIL et al, 2007), Argentina (CALDERON et al, 2009), Brazil (CASTRO et al, 2010 and Uruguay (PEREZ et al, 2007 The CPV variants differ in amino acid changes occurring at residue 426 of the capsid protein, with types CPV-2a, CPV-2b, CPV-2c displaying amino acids Asparagine (Asn), Asp and Glu, respectively (BUONAVOGLIA et al, 2001).…”

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

“…Only one study reports the complete protection by the original CPV type 2 based vaccines against challenge with any of the CPV circulating strains (SPIBEY et al, 2008). However, there are several researches that reports vaccine failure when the challenge virus is the CPV type 2c (DECARO et al, 2007;PEREZ et al, 2007;DECARO et al, 2008;CALDERON et al, 2009). It is important to note that most current cases of canine parvovirosis are related to the virulent strain CPV-2c in Argentina (CALDERON et al, 2009), Uruguay (PEREZ et al, 2007) and Brazil (STRECK et al, 2009).…”

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

“…However, there are several researches that reports vaccine failure when the challenge virus is the CPV type 2c (DECARO et al, 2007;PEREZ et al, 2007;DECARO et al, 2008;CALDERON et al, 2009). It is important to note that most current cases of canine parvovirosis are related to the virulent strain CPV-2c in Argentina (CALDERON et al, 2009), Uruguay (PEREZ et al, 2007) and Brazil (STRECK et al, 2009). All these cases take place mainly in previously immunized animals whose vaccination probably failed, and CPV-2c strain would not be neutralized by vaccine strain CPV-2.…”

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

“…Despite CPV-2c was detected causing recent cases of canine parvovirosis in South America by molecular characterization (PEREZ et al, 2007;CALDERON et al, 2009;STRECK et al, 2009), the viable infectious particle was not isolated yet. The present study reports the first isolation in cell culture of a CPV-2c strain in Uruguay.…”

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

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Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

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Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 72%

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

Section: Palavras-chave: Parvovirose Canina Cpv-2c Cães Vacinadosmentioning

confidence: 99%

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Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

Pintos

Larrama²,

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et al. 2011

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Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

et al. 2016

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Canine parvovirus type 2 (CPV-2) comprises three antigenic subtypes (2a, 2b and 2c) that have been reported in many countries. These subtypes cause serious disease in dogs with characteristic gastroenteritis signs. Little information has been documented in Africa about the genetic characterization of CPV-2. The aim of this study was to detect and to characterize the CPV-2 subtypes circulating in dogs admitted to Veterinary Clinics from two cities of Mozambique, Maputo and Matola, in 2010. A total of 40 field fecal samples were collected and tested for CPV-2 by polymerase chain reaction assay. The partial length VP2 gene of the positive samples were sequenced and genetically analyzed. Twenty-six (65%) fecal samples were positive for CPV-2. The restriction fragment length polymorphism analysis was also performed from positive samples and did not reveal the presence of CPV-2c subtype. The results of the sequencing revealed the presence of CPV-2a (n = 9) and CPV-2b (n = 17). No CPV-2 and CPV-2c were detected. Sequence analysis comparison showed nucleotide identities of 99.6-100% among our CPV-2 isolates. Amino acid analysis showed predicted amino acid changes. Phylogenetically, all of the CPV-2a strains isolated formed a cluster together with South African and Nigerian isolates. Most of Mozambican CPV-2b isolates also tended to cluster together with South African isolates; however, four were more closely related to French strain and one isolates to the American strain. The present study was the first to characterize the CPV-2 circulating in the Mozambican dog population.

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Detection and genetic characterization of canine parvoviruses and coronaviruses in southern Ireland

et al. 2010

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Canine parvovirus (CPV) and canine coronavirus (CCoV) are considered the main pathogens responsible for acute gastroenteritis in dogs. From a collection of 250 samples, seven CPV strains and three CCoV strains were identified in symptomatic Irish dogs. Samples were screened for the viruses using polymerase chain reaction (PCR) and typed via DNA sequence analysis. Three CPV strains were characterized as CPV-2a, while four others were characterized as CPV-2b. To date, CPV-2c remains unreported in Ireland. Two CCoV strains were characterized as CCoV-II and one as CCoV-I. In the case of one sample, PH4/09/Ire, a mixed infection with CPV and CCoV was detected.

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Molecular characterization of canine parvovirus strains in Argentina: Detection of the pathogenic variant CPV2c in vaccinated dogs

Cited by 61 publications

References 40 publications

Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

Detection and genetic characterization of canine parvoviruses and coronaviruses in southern Ireland

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