Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay

Pereira, César Augusto Dinóla; Monezi, Telma Alves; Mehnert, Dolores Ursula; D'Angelo, M.; Durigon, Edison Luíz

doi:10.1016/s0378-1135(00)00214-5

Cited by 109 publications

(117 citation statements)

References 16 publications

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“…CPV-2b was the predominant genotype, which is in agreement with the subtype found in the United Kingdom and in some European countries (Decaro & Buonavoglia 2012). This is also in agreement with Pereira et al (2000) and Castro et al (2011), who reported a predominance of CPV-2a in the years 1980 and 1995-2006, a situation that changed for fecal samples collected from 1990-1995 and 2007-2009, in which CPV-2b was the predominant subtype. In our study, only one dog was infected with the genotype CPV-2a and another one with CPV-2c.…”

Section: Discussionsupporting

confidence: 89%

“…CPV-2 is gradually being replaced in the canine population by new antigenic variants or biotypes, designated CPV-2a and CPV-2b (Decaro & Buonavoglia 2012) and by a third biotype, CPV-2c, which has been increasing since its identification in 2001 (Hoelzer & Parrish 2010, Decaro & Buonavoglia 2012. Since 1980, (Hagiwara et al 1980, Angelo et al 1988, there has been a clear spread of hemorrhagic enteritis caused by canine parvovirus (Pereira et al 2000, Castro et al 2007, Streck et al 2009) in Brazil, including in our region. Nevertheless, in recent years, infrequent cases of the disease have been observed, suggesting that eventually the routine vaccination of puppies would induce immunity and, consequently, the disappearance of clinical cases.…”

Section: Introductionmentioning

confidence: 99%

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Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

et al. 2016

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Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70's new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central--West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination test and those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in São Paulo region.INDEX TERMS: Canine parvovirus, PCR, viral type, dogs.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

et al. 2016

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“…The sequence of the primer pair CPV2ab was described earlier [11] and the sequence of the primer pair CPV-2b was of CPV-2b (F) 5 0 -CTTTA ACCTTCCTGTAACAG-3 0 and CPV-2b(R) 5 0 -ATAGTTA AATTGGTTATCTAC-3 0 [15]. CPV 2b types would respond to both the primer pairs and will generate two specific products at 681 and 427 bp whereas CPV-2a would react with only CPV-2ab primer pair and generate only one specific product at 681 bp.…”

Section: Detection Of the Presence Of Virus In Cell Culturementioning

confidence: 99%

“…The results of molecular typing by PCR assay in Brazil showed that out of the 38 isolates collected during the period from 1982 to 1995, showed that the relative prevalence of the antigenic types changed over time. The predominant strain found during 1980 was CPV-2a, which was substantially replaced by CPV-2b from 1990 to 1995 [15]. In the recent report from North Indian states Sanjukta et al [23] showed that the CPV-2b was the prevalent strain in North India states.…”

Section: Characterization Of Cell Culture Isolates By Multiplex Pcr Amentioning

confidence: 99%

Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

Parthiban

Mukhopadhyay²,

Panneer³

et al. 2011

Indian J Microbiol

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A total of 128 faecal samples/rectal swabs were collected from dogs showing signs of diarrhea/enteritis in and around Puducherry, South India. Eighteen clinical samples, showing high HA titre of 1:512 and above and positivity by polymerase chain reaction (PCR) with CPV2ab primers, were subjected to virus isolation in CRFK cell line. Of the 18 samples processed, 3 samples (16.6%) were positive for CPV and were confirmed by haemagglutination, dot-ELISA, and IFAT. The three cell culture isolates were characterized as CPV-2b types by multiplex PCR as well as by monoclonal antibody typing.

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“…Since the emergence of canine parvovirus (CPV) in the late 1970s, virus enteritis has become recognized as one of the most common causes of infectious diarrhea in puppies (Pollock and Coyne, 1993). Although parvovirus infection can be controlled by vaccination, several reports show CPV infection in puppies to be a significant cause of morbidity in Brazil (Homem et al, 1999;Cubel Garcia et al, 2000, 2002.…”

Section: Introductionmentioning

confidence: 99%

Clinical and epidemiological aspects of canine parvovirus (CPV) enteritis in the State of Rio de Janeiro: 1995 - 2004

Castro¹,

Miranda²,

Labarthe³

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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This paper relates the clinical and epidemiological aspects of canine parvovirus infection (CPV) in the State of Rio de Janeiro from April 1995 to March 2004. A total of 341 fecal samples were collected from up to 6-months-old puppies with gastroenteritis. The diagnosis of CPV infection was confirmed by hemagglutination/ hemagglutination inhibition tests, enzyme immunoassay, virus isolation in cell culture or polymerase chain reaction. One hundred and fifty-seven samples (46%) were positive for CPV. No correlation among sex, breed or age and the occurrence of CPV infection was observed. The classical signs of parvoviral enteritis (anorexia, lethargy, vomiting and hemorrhagic fluid diarrhea) were observed in 70% of CPV-positive and in 60% of CPV-negative puppies. Although CPV could be detected throughout the studied period, its occurrence was significantly higher from June to September and November to December. These results show that CPV is still circulating in the State of Rio de Janeiro.Keywords: canine parvovirus (CPV), Rio de Janeiro, clinical signs, enteritis RESUMO Este trabalho relata os aspectos clínicos e epidemiológicos da infecção pelo CPV no Estado do Rio de

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Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay

Cited by 109 publications

References 16 publications

Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

Clinical and epidemiological aspects of canine parvovirus (CPV) enteritis in the State of Rio de Janeiro: 1995 - 2004

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