Molecular characterization of Brucella species from Zimbabwe

Ledwaba, Maphuti Betty; Gomo, Calvin; Lekota, Kgaugelo Edward; Flèche, Philippe Le; Hassim, Ayesha; Vergnaud, Gilles; Heerden, Henriëtte van

doi:10.1371/journal.pntd.0007311

Cited by 28 publications

(21 citation statements)

References 53 publications

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“…strains were isolated from seven areas of the examined region. It was important to follow up on the control strategies and implement prevention measures for the identification and typing of Brucella isolates [27]. Subsequently, these strains were identified and typed using classical biotyping methods and molecular schedules.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Liu

Wang²,

et al. 2020

Emerging Microbes & Infections

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In the present study, a total of 1102304 serum samples were collected to detected human brucellosis between the years 2012 and 2016 in Inner Mongolia. Overall, an average of 3.79% anti-Brucella positive in Inner Mongolia was presented but the range of positive rates were among 0.90 to 7.07% in 12 regions. Seroprevalence of human brucellosis increased gradually from 2012 to 2016. However, the incidence rate of human brucellosis showed a declining trend. One hundred and seven Brucella strains were isolated and identified as B. melitensis species, and B. melitensis biovar 3 was the predominant biovar. MLVA-11 genotypes 116 was predominant and had crucial epidemiology to the human population. All 107 strains tested were sorted into 75 MLVA-16 genotypes, with 54 single genotypes representing unique isolates. This result revealed that these Brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 21 shared genotypes among two to four strains, confirming the occurrence of crossinfection and multiple outbreaks. Extensive genotype-events were observed between strains from this study and Kazakhstan, Mongolia, and Turkey, these countries were key members of the grassland silk road. Long-time trade in small ruminants (sheep) in these countries has possibly promoted the spread of Brucella spp. in these regions.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Liu

Wang²,

et al. 2020

Emerging Microbes & Infections

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“…Multiple-locus variable-number tandem-repeat analysis (MLVA) has high power to discriminate closely related strains and can be used for tracing infections [15], achieves result largely in agreement with WGS-SNP-based typing [16]. In addition, its low cost and fast results allow its use as a routine first-line assay [17]. Ma et al [18] reported that the Brucella strain in Qinghai was different from strains in other regions of the world, possibly owing to the unique geography, such as the high altitude, of the QTP.…”

Section: Introductionmentioning

confidence: 94%

“…Minimum spanning trees (MST) were constructed based on MLVA-11 (Table S2) and MLVA-16 (Table S3) data using BioNumerics 7.6 to investigate the geographic origin and genetic relatedness of strains. Phylogenetic analysis of representative strains (Table S4) was performed based on WGS-SNP using the maximum parsimony method [17], B. abortus bv.1 str. 9-941 used as the outgroup strain.…”

Section: Dna Preparation Genotyping and Data Analysismentioning

confidence: 99%

Brucella melitensis , a latent “travel bacterium,” continual spread and expansion from Northern to Southern China and its relationship to worldwide lineages

Zhu

Zhao

et al. 2020

Emerging Microbes & Infections

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Brucellosis caused by Brucella melitensis is considered to be one of the most important zoonotic diseases in China. In this study, Conventional bio-typing, MLVA (multiple locus variable-number tandem repeat analysis), and WGS (whole-genome sequencing)-SNP (single nucleotide polymorphism) were used to study the genetic similarity of B. melitensis in northern and southern China and analyze its relationship with worldwide lineages. Currently, the distribution of species/biovars of B. melitensis has obviously changed, and B. melitensis has become the dominant species in southern regions of China. Strains from the southern had a common geographic origin with strains from the northern. Many MLVA-16 events were shared in the genotypes of the southern and northern strains, suggest that genotypic movement occurred from north to south. Based on WGS-SNP analysis, strains from different provinces were closely related and may have descended from one common ancestor, suggests that the southern strains originated from northern China. These data indicate that B. melitensis is a latent "travel bacterium" that spread and expanded from North China to South China. Moreover, B. melitensis strains from China are also genetically related to strains from other Asian regions (Kazakhstan, Russia, Mongolia, and India). The movement of infected sheep and their products requires control.

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“…In Hainan Province, which is located at the southernmost part of China, there were no historically reported brucellosis cases, and only one human brucellosis case was reported in Danzhou in 1985, brucellosis cases significantly increased in 2017 between humans (Wang et al, 2019), but the available information on the extent of the disease and relatedness among strains is unknown. Characterizing the circulating strains is a critical sector in understanding brucellosis in the epidemic area (Ledwaba et al, 2019). Accurate discrimination relatedness among strains with the MLVA assay is necessary to determine the source, origin, and geographical spread of infection (Whatmore et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Brucella Isolates Collected From Humans in Hainan Province, China

Wang

Zhu

et al. 2020

Front. Microbiol.

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Brucellosis has been reported in several regions of Hainan Province, but the extent of the disease has not been fully elucidated. Conventional biotyping methods, multiple locus variable number tandem repeats analysis (MLVA), and single-nucleotide polymorphisms (SNPs) from draft genome sequencing were employed to characterize the strains. There were four biovars (Brucella melitensis bv. 1, 2, and 3 and Brucella suis bv. 3) detected, which showed that the biovar diversity of Brucella in Hainan is higher than in other areas of China. Both B. melitensis bv. 3 and B. suis bv. 3 were dominant species and showed epidemiology patterns that were compatible with both southern and northern China. Eight of MLVA-11 genotypes were known (31, 111, 116, 120, 136, 291, 297, and 345), and the remaining seven were novel (HN11-1 to HN11-7); these data showed that Brucella strains in this study had multiple geographic origins and exhibited characteristics of origin and evolution of co-existing imported and Hainan specific lineage. A total of 41 strains were found, belonging to 37 unique genotypes that each represented a single strain, which suggests that these strains were not directly related epidemiologically and indicates that the epidemic characteristics of human brucellosis in Hainan was dominated by sporadic strains. The high HGDI values were observed in MLVA-8, MLVA-11, and MLVA-16 among two species, suggesting considerable genetic diversity among these species. MST is characterized based on MLVA-16 that was found both throughout China and on a global level and showed that strains of this study had significant genetic differences with strains from many parts of the globe and seemingly represent a unique genetic lineage. Whole-genome SNP analysis showed that four B. melitensis were closely related to strains from China's northern provinces, and the source of infection was partly of human brucellosis in this province that may have been from these regions. The B. suis were closely related to strains from the United States, and further investigation of the transportation of animals, such as pigs, is needed to elucidate the origins of these strains.

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Molecular characterization of Brucella species from Zimbabwe

Cited by 28 publications

References 53 publications

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Brucella melitensis , a latent “travel bacterium,” continual spread and expansion from Northern to Southern China and its relationship to worldwide lineages

Molecular Characteristics of Brucella Isolates Collected From Humans in Hainan Province, China

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