Molecular characterization of bovine amniotic fluid derived stem cells with an underlying focus on their comparative neuronal potential at different passages

Nawaz, Shah; Akkaya, Özlem; Dikmen, Tayfun; Altunbaş, Korhan; Yağcı, A.; Kibria, A. S. M. G.; Erdoğan, Metin; Çelik, Hacı Ahmet

doi:10.1016/j.aanat.2019.151452

Cited by 12 publications

(4 citation statements)

References 41 publications

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“…Adipogenic differentiation was proved by Oil red-O to detect intracellular lipid droplets ensuring that the cells differentiated into adipogenic lineage. These findings and procedures were similar to previous studies in this regard (Koç et al 2008, Moonesi Rad et al 2019, Fink and Zachar 2011, Nawaz et al 2020, Ozden-Akkaya et al 2019. Furthermore, neurospheres generation from adipose tissue make provision for the treatment of alleviation of neurogenic disorder or diseases that may need proper administration media and process.…”

Section: Discussionsupporting

confidence: 88%

Equine adipose tissue derived stem cells and their multilineage differentiation

Kibria

Nawaz

Dikmen

et al. 2020

Kocatepe Veterinary Journal

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Mesenchymal stem cells (MSCs) have become a potent source for cell therapy due their inherent properties of self-renewal and ability to undergo unlimited divisions in the field of regenerative medicine. In this study, equine adipose tissue stem cells (EASCs) have been evaluated on the basis of their morphology, proliferation potential and multilineage differentiation capabilities for their future usage in cell therapy. The cells were further differentiated into osteogenic and adipogenic cell lineages as well as into adipose derived neurospheres to ensure the stemness of propagated cells. In our study, we further successfully cultured the neurosphere derived cells which were cultured from neurospheres after enzymatic dissociation. The study exhibits that EASCs offer a suitable choice for cellular regenerative therapy in equines. Nonetheless, there is a still need to molecular characterization of EASCs in order to establish a standard practice in equine medicine.

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Section: Discussionsupporting

confidence: 88%

Equine adipose tissue derived stem cells and their multilineage differentiation

Kibria

Nawaz

Dikmen

et al. 2020

Kocatepe Veterinary Journal

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“…Therefore, investigating as much stem cell source as we can could point out the best source and approach for the intended use of neurospheres. Previous attempts on neurospheres generation from bovine stem cells were performed using bovine adipose tissue derived stem cells and bovine amniotic fluid derived stem cells under serum-free induction conditions by our team, and in both studies similar to the current research, all the neurospheres were positive against Sox2, Nestin and ß-III Tubulin protein expressions (Akkaya et al, 2019;Nawaz et al, 2020). Yet, a previous neurogenic attempt made by Dueñas et al (2014) using β-mercaptoethanol treatments on bovine BM-MSCs could not lead to successful expression of neural stem cell markers like Nestin and MAP2.…”

Section: Discussionsupporting

confidence: 78%

“…As all in vivo applications require the serum-free inoculation of stem cells and their derivatives, testing a serum-free approach for generating neurospheres is crucial for road mapping the futuristic approaches in the same area. With this point of view, we previously tested the potential of bovine adipose derived stem cells (Akkaya et al, 2019) and amniotic fluid derived stem cells (Nawaz et al, 2020) to generate Sox2 and ß-III Tubulin positive neurospheres using serum-free induction medium. In this study, an another source was used as bovine bone marrow derived mesenchymal stem cells were isolated.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bovine Bone Marrow Derived Mesenchymal Stem Cells and Immunostaining of Differentiated Neurospheres

Dikmen,

Özden Akkaya,

Nawaz

et al. 2023

J Hellenic Vet Med Soc

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Stem cell differentiation has been a key element for the therapeutical applications for various cases. The novel anti-inflammatory and angiogenic findings and potential applications for both autologous and allogenic studies have emphasized about the usage of mesenchymal stem cells in the clinical trials. The neurosphere differentiation studies and their role for peripheral nerve injuries have gained momentum. Following study emphasized on the isolation of stem cells from bovine bone marrow, their characterization by PCR and their differentiation into mesodermal lineages and into neurospheres under specific induction media. The generated neurospheres were durther investigated for their potential expressions of neuroprogenitor markers Sox2, Nestin and neurofilament protein β-III Tubulin by immunofluorescence staining. The results of following study highlight the use of bone marrow derived mesenchymal stem cells for translational research and regenerative therapy.

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“…CD271 was found to be mainly expressed by adult MSCs, whereas NG2 is predominantly expressed by fetal MSCs 8 . P1 AFMSCs present higher Nestin expression than P3 and P5 AFMSCs, and P5 AFMSCs show higher capacity to differentiate into neurospheres than P3 AFMSCs 27 . In our study, we observed that P48 AFMSCs presented higher Abca4 expression than P4, P7, and P22 AFMSCs.…”

Section: Discussionsupporting

confidence: 51%

miR-351-3p Promotes Rat Amniotic Fluid-Derived Mesenchymal Stromal Cell Proliferation via Targeting the Coding Sequence of Abca4

et al. 2021

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Amniotic fluid-derived mesenchymal stromal cells (AFMSCs) present different features, depending on the isolation timing and culture conditions. The lack of uniform experimental standards hinders the comparison of results from different studies on AFMSCs. Moreover, understanding the molecular mechanisms that underlie the features of AFMSCs isolated at different embryonic developmental stages might allow the obtention of more viable and highly proliferative AFMSCs through genetic modification. We isolated AFMSCs from pregnant rats at embryonic day (E)12, E15, E18, and E21 and compared their cell proliferation capacity and transcriptome. The cell counting kit-8 assay and RNA sequencing revealed that E12 and E15 AFMSCs showed different characteristics from E18 and E21 AFMSCs. Therefore, AFMSCs were divided into two groups: early (E12 and E15) and late (E18 and E21) pregnancy-stage groups. Next, we screened the gene/microRNA pair Abca4/miR-351-3p that was related to cell proliferation. Abca4 knockdown/overexpression suggested that this gene represses the proliferation of AFMSCs, which is a newly discovered function of this gene. Finally, dual luciferase reporter gene assays confirmed that miR-351-3p targeted the coding sequence of Abca4 and regulated AFMSC proliferation. miR-351-3p promotes AFMSC proliferation via targeting the coding sequence of Abca4. Our findings provide a molecular foundation for further research for obtaining AFMSCs with a higher proliferation capacity.

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Molecular characterization of bovine amniotic fluid derived stem cells with an underlying focus on their comparative neuronal potential at different passages

Cited by 12 publications

References 41 publications

Equine adipose tissue derived stem cells and their multilineage differentiation

Equine adipose tissue derived stem cells and their multilineage differentiation

Characterization of Bovine Bone Marrow Derived Mesenchymal Stem Cells and Immunostaining of Differentiated Neurospheres

miR-351-3p Promotes Rat Amniotic Fluid-Derived Mesenchymal Stromal Cell Proliferation via Targeting the Coding Sequence of Abca4

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