Amniotic membrane derived stem cells (AMSCs) are reported to have a comparatively higher potency than multipotent stem cells. These cells are shown to have low immunogenicity and no teratogenicity. Among various conventional methods of isolation using enzymes, explant culture method is believed to be an easy and costeffective way to harvest stem cells. The purpose of this study was to isolate AMSCs from amniotic membrane of rats and to characterize them for multilineage differentiation, including generation of neurospheres to use them later in in-vivo experiments. Amniotic membranes were collected from Wistar rats on 17 th day of pregnancy. After processing of the tissues, AMSCs were isolated by the explant culture method and continued to grow until 10 th passage. The doubling time was estimated and the cells were analyzed for growth curve parameters at passages 5 and 9. The osteogenic and adipogenic differentiation studies were carried out from the same cells after 3 rd passage.Neurospheres generation from AMSCs was performed using neurogenic induction media.The cells were further assessed for their mesenchymal, haemopoietic, and neurogenic marker expressions by immunofluorescence staining and PCR analysis The study suggests that AMSCs isolated through explant culture are reliable stem cells which could generate neurospheres under proper induction conditions and could be a potential candidate to be used on in-vivo neural degeneration models.
Drinking water from natural sources in coastal region of Bangladesh has been contaminated by varying degrees of salinity which have detrimental effects on the health. Pregnancy itself is a crucial time for women and the salinity increases its vulnerability regarding the raising of blood pressure. The study was conducted to evaluate the effect of saline water intake on the health status of pregnant women at Barguna and Patuakhali districts, Bangladesh. Sodium (Na) concentration in urine samples was analyzed and corresponding blood pressure was taken in the diagnostic laboratory. Descriptive statistical analysis showed that Na concentration, systolic and diastolic pressure (BP) was the highest in 2nd trimester followed by 3rd trimester and 1st trimester. Water intake and urine production were the highest in 3rd trimester and increased with age. Na concentration, systolic and diastolic pressure (bp) was the highest in 3rd parity followed by 2nd parity. Water intake and urine production did not very among parities. Correlation matrix express that sodium concentration has the significant association with the age and the highly significant association with systolic and diastolic blood pressure. In Logistic Regression Model, odds ratio (OR) estimate showed that women with systolic bp?120 had 1.109 times greater sodium concentration than with systolic blood pressure of <120 and, with diastolic bp ? 80 contain 1.064 times greater sodium concentration than with diastolic blood pressure of < 80.Asian J. Med. Biol. Res. March 2016, 2(1): 55-61
Mesenchymal stem cells (MSCs) have become a potent source for cell therapy due their inherent properties of self-renewal and ability to undergo unlimited divisions in the field of regenerative medicine. In this study, equine adipose tissue stem cells (EASCs) have been evaluated on the basis of their morphology, proliferation potential and multilineage differentiation capabilities for their future usage in cell therapy. The cells were further differentiated into osteogenic and adipogenic cell lineages as well as into adipose derived neurospheres to ensure the stemness of propagated cells. In our study, we further successfully cultured the neurosphere derived cells which were cultured from neurospheres after enzymatic dissociation. The study exhibits that EASCs offer a suitable choice for cellular regenerative therapy in equines. Nonetheless, there is a still need to molecular characterization of EASCs in order to establish a standard practice in equine medicine.
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