Molecular characterization of border disease virus strain Aveyron

Vilček, Štefan; Leskova, Valeria; Meyer, Denise; Postel, Alexander; Becher, Paul

doi:10.1016/j.vetmic.2014.03.028

Cited by 9 publications

(7 citation statements)

References 28 publications

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“…Border disease virus (BDV), is an important infectious agent in sheep and goats causing serious economic losses (1). Four species of the genus Pestivirus within the family Flaviviridae have been recognized including BDV of sheep and goats, bovine viral diarrhea virus 1 and 2 of cattle and classical swine fever virus of swine (2). Novel Pestivirus species, like Giraffe virus, Pronghorn virus, Bungowannah virus and HoBi-like virus were isolated (3,4).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Hassan¹

2020

IJVS

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Globally, Border disease virus (BDV) has caused substantial economic losses among small ruminants (sheep and goats). This is the first molecular study carried out in Mosul city, Iraq. To determine the prevalence of Border Disease Virus and to examine problem of persistent infection (PI) using Reverse transcriptase polymerase chain reaction technique (RT-PCR) in female local breed of small ruminants. During the period between November 2018 to June 2019, 364 blood samples were collected from 264 local Awassi sheep and 100 local cross breed goats secure provided by private breeders. The animals were of ≥1.5 years old and the samples were obtained from various locations in Mosul city, with varying rearing methods and had not been vaccinated against BDV. This investigation indicated that the prevalence of BDV infection in sheep and goats were 15.9% (42/264) and 3% (3/100) respectively, whereas the occurrence of PI in sheep was 2.38% and in goats was 0%. Hence it was concluded that Border disease was circulating in small ruminants in Mosul city. This calls for a need to design programs to monitor and control the disease and eventually eradicate it is prevalence in Mosul city.

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Section: Introductionmentioning

confidence: 99%

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Hassan¹

2020

IJVS

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“…Pestivirus is a genus within family Flaviviridae , comprises four recognized species, namely border disease virus (BDV) of small ruminants, bovine viral diarrhea viruses 1 and 2 (BVDV-1, BVDV-2) of cattle and classical swine fever virus (CSFV) of pigs, respectively [ 1 ]. Recently, new pestivirus species, such as Giraffe virus, Pronghorn virus, Bungowannah virus and HoBi-like virus have been discovered [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of one sheep border disease virus in China

Liu

et al. 2015

Virol J

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BackgroundBorder disease virus (BDV) causes border disease (BD) affecting mainly sheep and goats worldwide. BDV in goat herds suffering diarrhea was recently reported in China, however, infection in sheep was undetermined. Here, BDV infections of sheep herds in Jiangsu, China were screened; a BDV strain was isolated and identified from the sheep flocks in China. The genomic characteristics and pathogenesis of this new isolate were studied.ResultsIn 2012, samples from 160 animals in 5 regions of Jiangsu province of China were screened for the presence of BDV genomic RNA and antibody by RT-PCR and ELISA, respectively. 44.4% of the sera were detected positively, and one slowly grown sheep was analyzed to be pestivirus RNA positive and antibody-negative. The sheep kept virus positive and antibody negative in the next 6 months of whole fattening period, and was defined as persistent infection (PI). The virus was isolated in MDBK cells without cytopathic effect (CPE) and named as JSLS12-01. Near-full-length genome sequenced was 12,227 nucleotides (nt). Phylogenetic analysis based on 5'-UTR and Npro fragments showed that the strain belonged to genotype 3, and shared varied homology with the other 3 BDV strains previously isolated from Chinese goats. The genome sequence of JSLS12-01 also had the highest homology with genotype BDV-3 (the strain Gifhorn). Experimental infections of sheep had mild clinical signs as depression and short-period mild fever (5 days). Viremia was detected in 1–7 days post-infection (dpi), and seroconversion began after 14 dpi.ConclusionsThis study reported the genomic and pathogenesis characterizations of one sheep BDV strain, which confirmed the occurrence of BDV infection in Chinese sheep. This sheep derived BDV strain was classified as BDV-3, together with the goat derived strains in China. These results might be helpful for further understanding of BDV infection in China and useful for prevention and control of BDV infections in the future.

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“…Like other RNA viruses, pestiviruses also exhibit genetic heterogeneity which has been supported by several workers [ 10 - 16 ]. Comparative sequence analysis of CSFV/IND/UK/LAL-290 exhibited analogous genomic organization to those from reference pestivirus strains.…”

Section: Resultsmentioning

confidence: 84%

“…During the past decade, much progress on the molecular characterization of pestiviruses has been achieved. Although, few isolates have been sequenced completely, and comparative studies on full genome sequence are scanty [ 10 - 13 ]. Generation of complete genome sequence data will help in better understanding of the molecular biology of any virus both at structural and functional level.…”

Section: Introductionmentioning

confidence: 99%

Sequence-based comparative study of classical swine fever virus genogroup 2.2 isolate with pestivirus reference strains

et al. 2015

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Aim:This study was undertaken with the aim to compare and establish the genetic relatedness between classical swine fever virus (CSFV) genogroup 2.2 isolate and pestivirus reference strains.Materials and Methods:The available complete genome sequences of CSFV/IND/UK/LAL-290 strain and other pestivirus reference strains were retrieved from GenBank. The complete genome sequence, complete open reading frame, 5’ and 3’ non-coding region (NCR) sequences were analyzed and compared with reference pestiviruses strains. Clustal W model in MegAlign program of Lasergene 6.0 software was used for analysis of genetic heterogeneity. Phylogenetic analysis was carried out using MEGA 6.06 software package.Results:The complete genome sequence alignment of CSFV/IND/UK/LAL-290 isolate and reference pestivirus strains showed 58.9-72% identities at the nucleotide level and 50.3-76.9% at amino acid level. Sequence homology of 5’ and 3’ NCRs was found to be 64.1-82.3% and 22.9-71.4%, respectively. In phylogenetic analysis, overall tree topology was found similar irrespective of sequences used in this study; however, whole genome phylogeny of pestivirus formed two main clusters, which further distinguished into the monophyletic clade of each pestivirus species. CSFV/IND/UK/LAL-290 isolate placed with the CSFV Eystrup strain in the same clade with close proximity to border disease virus and Aydin strains.Conclusion:CSFV/IND/UK/LAL-290 exhibited the analogous genomic organization to those of all reference pestivirus strains. Based on sequence identity and phylogenetic analysis, the isolate showed close homology to Aydin/04-TR virus and distantly related to Bungowannah virus.

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Molecular characterization of border disease virus strain Aveyron

Cited by 9 publications

References 28 publications

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

Characterization of one sheep border disease virus in China

Sequence-based comparative study of classical swine fever virus genogroup 2.2 isolate with pestivirus reference strains

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