Molecular characterization of antimicrobialLactobacillusisolates and evaluation of their probiotic characteristicsin vitrofor use in poultry

Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics

“…Antagonistic activity by LAB are sustained by the secretion of different antimicrobial substances including organic acids (lactic, acetic etc), bacteriocins, alcohols, hydrogen peroxide, antimicrobial peptide etc. [17]. The substances responsible for the antagonistic activity by most promising LAB probiotic strains selected as revealed in our study were organic acid and low molecular weight substances.…”

“…Infections by zoonotic and foodborne enteric pathogens cause high morbidity and mortality with significant economic loss in the poultry industry [17]. Also, these pathogens are often transmitted to humans either via occupational exposure or through the food chain, which is of immense public health concern.…”

“…These are 2 of the several factors involved in probiotics adhesion in chicken intestinal cells [45]. Whereas autoaggregation is an important requirement for the formation of biofilm which further aid adhesion and colonization of host intestinal cells by probiotics, coaggregation on the other hand enables probiotics to form barrier that is effective in the prevention of enteric pathogens adhesion on intestinal cells [17]. The results of autoaggregation abilities by LAB strains as recorded from our study was between 32 ± 5.66 to 56.5 ± 3.54% (Table 4).…”

“…The coaggregation abilities between the potential LAB probiotics and the 6 pathogens tested showed strain-and-pathogen specific coaggregation abilities, ranging between 24.03 ± 0.04 (for E. faecalis ) to 83.6 ± 0.83% (for L. monocytogenes ). Findings from the work of Venkatasatyanarayana et al [17] reported 62.2 ± 1.03% and 35.5 ± 1.32% coaggregation between L. plantarum to E. coli and L. monocytogenes .…”

“…The assessment of antimicrobial susceptibility profile is a major criterion for potential probiotics evaluation. Microbial strains to be considered as probiotics should not serve as antibiotic resistance genes reservoir, which may further be transferred to intestinal pathogens [17]. All the 6 (100.00%) LAB isolates were susceptible to penicillin, 5 (83.33%) were susceptible to chloramphenicol while 4 (66.67%) were susceptible to ceftriaxone, ampicillin, and novobiocin (Tables 5 and 6).…”