Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics

Reuben, Rine Christopher; Roy, Pravas Chandra; Sarkar, Shovon Lal; Alam, A. S. M. Rubayet Ul; Jahid, Iqbal Kabir

doi:10.1186/s12866-019-1626-0

Cited by 191 publications

(176 citation statements)

References 58 publications

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“…Human large intestine contains bile salt, whose concentration varies between 0.3%~0.5%. Generally, the pH of gastric juice depends on the time of feeding, diet and it may vary from 1.5 to 4.5 [43,44]. Growth and survival at low pH and in the presence of bovine bile are thought to be the most desirable characteristics for future probiotic strains.…”

Section: Discussionmentioning

confidence: 99%

Functional probiotics of lactic acid bacteria from Hu sheep milk

Chen

Wang

et al. 2020

BMC Microbiol

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Background: Probiotics are being considered as valuable microorganisms related to human health. Hu sheep is referred as one of the important sheep breeds in China. Goat milk produced by Hu sheep is characterized with high nutritional value and hypoallergenic in nature. Particularly, this milk contains plenty of milk prebiotic and probiotic bacteria. This study was aimed to scrutinize more bacterial strains from Hu sheep milk with potential probiotic activity. Results: Based on 16S rRNA sequence analysis, pool of forty bacterial strains were identified and evaluated their antimicrobial activities against Staphylococcus aureus, enterohemorrhagic Escherichia coli (EHEC), Salmonella typhimurium, Listeria monocytogenes enterotoxigenic E. coli (ETEC) and Aeromonas caviae. Four out of these isolated strains demonstrated their efficient bacteriostatic ability and potential healthy properties. We also examined the safety aspects of these bacterial candidates including three Lactococcus lactis strains (named as HSM-1, HSM-10, and HSM-18) and one Leuconostoc lactis strain (HSM-14), and were further evaluated via in vitro tests, including antimicrobial activity, cell surface characteristics (hydrophobicity, co-aggregation, and self-aggregation), heat treatment, antibiotic susceptibility, simulated transport tolerance in the gastrointestinal tract, and acid/bile tolerance. The obtained results revealed that HSM-1, HSM-10, HSM-14, and HSM-18 showed high survival rate at different conditions for example low pH, presence of bovine bile and demonstrated high hydrophobicity. Moreover, HSM-14 had an advantage over other strains in terms of gastrointestinal tract tolerance, antimicrobial activities against pathogens, and these results were significantly better than other bacterial candidates.

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Section: Discussionmentioning

confidence: 99%

Functional probiotics of lactic acid bacteria from Hu sheep milk

Chen

Wang

et al. 2020

BMC Microbiol

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“…36 pathogenic bacteria in poultry, which in turn supports their use in improving the poultry performance and prevents transmission of poultry pathogens to humans. 37 We employed different strategies to confirm the inhibitory activity of the CFS. First, the classical well diffusion test was used as a screening tool.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Activities of Cell-Free Supernatants of Lactobacilli Against Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae and Pseudomonas aeruginosa

El‐Mokhtar¹,

Hassanein²,

Ahmed³

et al. 2020

IDR

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This study aimed to describe the inhibitory activity of cell-free supernatants (CFS) of lactobacilli against extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (K pneumoniae) and Pseudomonas aeruginosa (P aeruginosa). Material and Methods: Pathogenic clinical strains of K pneumoniae and P aeruginosa were isolated from urine samples and selected for investigation. Anti-bacterial activities of the CFS of lactobacilli were assessed by agar well diffusion, MTT assay, as well as time-kill tests. In addition, the antibiofilm characteristics were analyzed by the microplate method against fresh and 24 hold biofilms. The ability of CFS to interfere with bacterial invasion was analyzed by flow cytometry. Results: Although all tested strains were ESBL producers and showed a multidrug-resistant phenotype, the CFS displayed a high anti-ESBL activity with inhibition zone diameters greater than 13 mm in the agar well diffusion assays against both pathogens. The growth kinetics of K pneumoniae and P aeruginosa were dramatically decreased in the presence of the CFS. The CFS not only inhibited the biofilm formation by these pathogens but also was able to remove the 24-h formed biofilms. The invasion abilities of FITC-labelled K pneumoniae decreased from 30.3%±7 to 15.4%±5 and invasion of FITC-labelled P aeruginosa was reduced from 36.9%±7 to 25.2%±5. Conclusion: CFS of lactobacilli exhibit anti-ESBL activities, which suggests its potential application for controlling or preventing colonization of infections caused by ESBL-producing bacteria.

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“…L. acidophilus was reported the antibacterial activity against L. monocytogenes in cheese ( Ehsani et al., 2019 ). Enterococcus faecium isolated from poultry gastrointestinal tract could be used as a potential probiotic for preventing L. monocytogenes infection ( Reuben et al., 2019 ), and E. faecium selected from rabbit faeces showed an inhibitory activity against E. avium , L. innocua , and L. monocytogenes ( Simonová and Lauková, 2007 ). The anti-listerial activity of L. plantarum was via bacteriocin production and adhesion properties in vitro and in mice ( Van Zyl et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Possible action mechanisms of probiotics are involved in the modulation of intestinal barrier, defensin, inflammation, short-chain fatty acids, as well as cell interactions with hosts and pathogens ( Markowiak and Śliżewska, 2017 , Wang et al., 2019a , Wang et al., 2019b ). In recent studies, probiotic Lactobacillus plantarum and Lactobacillus acidophilus have been shown a significant anti-listerial activity ( Ehsani et al., 2019 , Reuben et al., 2019 , Van Zyl et al., 2019 , Zhao et al., 2020 ). However, whether probiotics influence the activities of virulence factors of pathogens including L. monocytogenes remains mostly unclear.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers

et al. 2020

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The present study aimed to investigate the effect of probiotic Lactobacilli addition on Listeria monocytogenes load, inflammatory reaction, and virulence properties in broilers from 1 to 14 D of age. A total of 480 broiler chicks were randomly allocated to 4 treatments of 6 replicates each. All birds were infected with L. monocytogenes on the first day and supplemented an equal amount mixture of Lactobacillus acidophilus and Lactobacillus plantarum at doses of 0 (control), 10 6 , 10 8 , 10 10 cfu/kg of diet. The results showed that on 7 and 14 D after administration, Lactobacilli addition at the 3 doses decreased ( P < 0.05) L. monocytogenes loads in the cecum, skin, liver, and spleen by 0.065 to 0.933 log 10 cfu, and the pathogen linearly reduced ( P ≤ 0.015) with the increasing doses of probiotics in the skin. Serum cytokines including IL-1β, IL-6, tumor necrosis factor-α, and interferon-γ in probiotics treatments were decreased ( P < 0.05) by 25.4 to 51.1%. Transcriptional levels of genes related to anti-inflammatory reactions including IL-10, hypoxia inducible factor 1 alpha ( HIF1A ), prostaglandin E receptor 2, and prostaglandin-endoperoxide synthase 2 in the intestinal mucosa were upregulated ( P < 0.05) in Lactobacilli treatments, and linear and quadratic responses ( P ≤ 0.019) were found on HIF1A. Furthermore, the probiotics attenuated ( P < 0.05) listerial adhesion, pore-forming, and invasion properties by downregulating autolysin Ami, listeriolysin O, internalin A and B, and a linear ( P = 0.006) dose response of probiotics was exhibited on flagellin. The findings indicate that dietary coadministration of L. acidophilus and L. plantarum can attenuate L. monocytogenes infection by depressing its intestinal inoculation, translocation, inflammatory reaction, and virulence property in broilers and suggest that the probiotics can be an alternative against listerial infection in broilers.

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Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics

Cited by 191 publications

References 58 publications

Functional probiotics of lactic acid bacteria from Hu sheep milk

Functional probiotics of lactic acid bacteria from Hu sheep milk

<p>Antagonistic Activities of Cell-Free Supernatants of Lactobacilli Against Extended-Spectrum β-Lactamase Producing <em>Klebsiella pneumoniae</em> and <em>Pseudomonas aeruginosa</em></p>

Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers

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