Molecular characterization of antibiotic resistant Vibrio harveyi isolated from shrimp aquaculture environment in the south east coast of India

Stalin, Nattan; Srinivasan, Pappu

doi:10.1016/j.micpath.2016.05.021

Cited by 64 publications

(24 citation statements)

References 54 publications

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“…The presence of antibiotics in water can cause serious environmental issues, such as the emergence of resistance due to selective pressure (Baquero et al, 2008). Recently, several studies have reported the development of multiple antibiotic resistance in the microbes of the aquaculture systems (Stalin and Srinivasan, 2016;Prichula et al, 2016;Uma and Ronald, 2016). Although, most of the studies on antibiotic resistance and virulence of Enterococci sp have been carried out in strain isolated from clinical samples, recent reports have suggested that environment and food could play a significant role in the transmission of resistance to humans (Gomes et al, 2008;Koluman, 2009;Barbosa et al, 2010;Chajęcka -Wierzchowska et al, 2016).…”

Section: Antibiotic Susceptibility Profilementioning

confidence: 99%

Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia

Uma¹,

Stalin²,

Rebecca³

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Antibiotic Susceptibility Profilementioning

confidence: 99%

Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia

Uma¹,

Stalin²,

Rebecca³

2017

Int.J.Curr.Microbiol.App.Sci

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“…The widespread use of antibiotics to control diseases in aquaculture not only increases the pressure on the microbial community but also promotes the emergence of bacterial resistance (Stalin & Srinivasan, ). Bacteria can acquire resistance through the transfer of mobile genetic elements (such as plasmids, phages, integrons and transposons) and horizontal transfer of antibiotic resistant shapes (Rocha & Sousa, ; You, Bong, & Lee, ).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, MDR V. harveyi has been very common in seafood. Most V. harveyi obtained by Nattan Stalin (Stalin & Srinivasan, ) from shrimp aquaculture waters along the southeastern coast of India were resistant to antibiotics and highly resistant to ampicillin, cefaclor, ciprofloxacin, penicillin, rifampicin and vancomycin. ZM Zhu (Zhu, Dong, Weng, & He, ) indicated that MDR V. harveyi is also highly prevalent in crossbred groupers on the southeastern coast and the northeastern coast of China.…”

Section: Discussionmentioning

confidence: 99%

“…However, with the abuse of antibiotics, bacterial drug resistance has become an extremely serious worldwide problem (Hsu et al, ). According to the survey, V. harveyi in mariculture waters of China has severe multidrug resistance, and the drug resistance spectrum is diverse (Stalin & Srinivasan, ). At present, the drug resistance of culture source V. harveyi to penicillins, macrolides, aminoglycosides and sulpha antibiotics becomes more serious.…”

Section: Introductionmentioning

confidence: 99%

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Honokiol inhibitsVibrio harveyihemolysin virulence by reducing its haemolytic activity

Guo

Xing

et al. 2019

Aquac Res

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Vibrio harveyi, as one of the main pathogens of vibriosis, has serious multidrug resistance, which has been a focus for domestic and foreign researchers. Vibrio harveyi hemolysin (VHH), an important virulence determinant of V. harveyi, causes cell necrosis and tissue damage by lysing cell membranes, which is essential for V. harveyi invasion of the host. The critical role of VHH in the virulence of V. harveyi renders it an ideal target for designing novel anti-virulence therapeutics. We found that honokiol, a natural compound, could act as a potent antagonist of VHH-mediated haemolysis at non-bacteriostatic concentrations. Honokiol can effectively inhibit the damage caused by VHH and V. harveyi both in vitro and in vivo. qRT-PCR and molecular docking analysis showed that although honokiol up-regulated the transcription level of vhh, it could block the active centre of VHH directly and forcefully, thereby reducing the catalytic activity of VHH. The results suggest that HNK could have great potential in the development of new aquaculture drugs. K E Y W O R D S anti-virulence, honokiol, VHH, Vibrio harveyi

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“…Setelah 30 menit disk antibiotic tersebut diletakkan pada permukaan plate agar kemudian diinkubasi selama 18-24 jam pada suhu 37 ºC. Setelah diinkubasi, zona hambat bakteri diukur dan dibandingkan dengan grafik interpretasi (Stalin & Srinivasan, 2016).…”

Section: Uji Sensitifitas Bakteri Terhadap Antibiotikunclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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Molecular characterization of antibiotic resistant Vibrio harveyi isolated from shrimp aquaculture environment in the south east coast of India

Cited by 64 publications

References 54 publications

Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia

Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia

Honokiol inhibitsVibrio harveyihemolysin virulence by reducing its haemolytic activity

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

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