Molecular Characterization of Antagonistic Streptomyces Isolated from a Mangrove Swamp

Kan, R. Rajesh; Prakash, Swadesh

doi:10.3923/ajbkr.2011.237.245

Cited by 17 publications

(2 citation statements)

References 6 publications

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“…Previous studies have shown that mangrove rhizosphere soils are rich in streptomycetes 77–80 . In the complex nature of mangrove rhizosphere environment, generation of root exudates could be the stimuli for species richness and chemical diversity of streptomycetes 78,79 . This rationalizes the higher number of Streptomyces isolates recovered from rhizosphere soil samples especially for those collected at sites KTTAS1, KTTAS2, and KTTAS3 as compared to that of sediment samples collected at site KTTAS6.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Streptomyces spp. from mangrove forest of Sarawak (Malaysia) and screening of their antioxidant and cytotoxic activities

Law

Chan

et al. 2019

Sci Rep

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Streptomycetes have been the center of attraction within scientific community owing to their capability to produce various bioactive compounds, for instance, with different antimicrobial, anticancer, and antioxidant properties. The search for novel Streptomyces spp. from underexplored area such as mangrove environment has been gaining attention since these microorganisms could produce pharmaceutically important metabolites. The aim of this study is to discover the diversity of Streptomyces spp. from mangrove in Sarawak and their bioactive potentials — in relation to antioxidant and cytotoxic activities. A total of 88 Streptomyces isolates were successfully recovered from the mangrove soil in Kuching, state of Sarawak, Malaysia. Phylogenetic analysis of all the isolates and their closely related type strains using 16S rRNA gene sequences resulted in 7 major clades in the phylogenetic tree reconstructed based on neighbour-joining algorithm. Of the 88 isolates, 18 isolates could be considered as potentially novel species according to the 16S rRNA gene sequence and phylogenetic analyses. Preliminary bioactivity screening conducted on the potential novel Streptomyces isolates revealed significant antioxidant activity and notable cytotoxic effect against tested colon cancer cell lines (HCT-116, HT-29, Caco-2, and SW480), with greater cytotoxicity towards SW480 and HT-29 cells. This study highlighted that the Sarawak mangrove environment is a rich reservoir containing streptomycetes that could produce novel secondary metabolites with antioxidant and cytotoxic activities.

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Section: Discussionmentioning

confidence: 99%

Diversity of Streptomyces spp. from mangrove forest of Sarawak (Malaysia) and screening of their antioxidant and cytotoxic activities

Law

Chan

et al. 2019

Sci Rep

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“…In recent years, marine actinomycetes have emerged as a rich source of novel compounds (4). The Streptomyces species isolated from mangrove environment showed divergent in their phylogenetic analysis and possessed good antibacterial and antifungal activities (5). This study therefore isolate and screen for the antimicrobial properties of actinomycetes from marine soil sediments and sponges in an attempt for a new, safe and effective antimicrobial agent.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Actinomycetes for Antimicrobial Activities from Marine Soil Sediments and Sponges

Kurup¹,

Ram²,

Kumari³

2016

West Indian Med J

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Objective: The aim of this study was to isolate and screen for the antimicrobial properties of actinomycetes from marine soil sediments and sponges. Methods: The samples of sponges and sediments were collected from various locations from the coast of Andhra Pradesh. Isolation of actinomycetes was done by plating them on starch casein agar, kuster's agar, Actinomycete isolation agar and potassium tellurite agar medium using dilution technique. Various pathogenic bacterial strains, fungal strains and yeasts were obtained from Microbial Type Culture

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Fungal cell-wall lytic enzymes, antifungal metabolite(s) production, and characterization fromStreptomyces exfoliatusMT9 for controlling fruit-rotting fungi

Choudhary

Nagpure

Gupta

2014

J. Basic Microbiol.

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An antifungal actinomycete strain MT9 was isolated from Loktak Lake, Manipur, India and its cultural characteristics, fatty acid methyl ester, 16S rRNA gene analysis suggests that strain MT9 is identical to Streptomyces exfoliatus. Strain MT9 displayed strong and broad-spectrum antagonism towards several fruit-rotting fungi by mycelial growth suppression. Crude fungal cell-wall lytic enzymes, i.e., chitinase, β-1,3-glucanase, and protease produced by S. exfoliatus MT9 were optimally active at pH 8.0 and 50 °C, pH 5.0 and 60 °C, pH 9.0 and 70 °C, respectively. All three mycolytic enzymes had good stability over a wide pH range of 5.0-10.0, with protease being more thermostable than both chitinase and β-1,3-glucanase. Interestingly zymogram analysis revealed that S. exfoliatus MT9 secretes six distinct chitinase isoenzymes with approximate molecular weights of 9.42, 13.93, 27.87, 36.43, 54.95, 103.27 kDa, six active protease isoenzymes with apparent molecular weights of 12.45, 30.20, 37.45, 46.32, 52.46, 131.46 kDa, and an active band of 119.39 kDa as β-1,3-glucanase enzyme. Extracellular fluid and its organic solvent extracts also exhibited inhibitory activity to various fruit-rotting fungi. The MIC value of n-butanol extract was 2-25 µg/ml against tested fruit-rotting fungi. Antifungal secondary metabolite(s) was found to be polyene in nature. To the best of our knowledge, this is the first report on extracellular production of fungal cell-wall lytic enzymes and antifungal metabolites by bioactive S. exfoliatus MT9 under submerged fermentation.

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Molecular Characterization of Antagonistic Streptomyces Isolated from a Mangrove Swamp

Cited by 17 publications

References 6 publications

Diversity of Streptomyces spp. from mangrove forest of Sarawak (Malaysia) and screening of their antioxidant and cytotoxic activities

Diversity of Streptomyces spp. from mangrove forest of Sarawak (Malaysia) and screening of their antioxidant and cytotoxic activities

Isolation and Screening of Actinomycetes for Antimicrobial Activities from Marine Soil Sediments and Sponges

Fungal cell-wall lytic enzymes, antifungal metabolite(s) production, and characterization fromStreptomyces exfoliatusMT9 for controlling fruit-rotting fungi

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