Molecular characterization of an IL-1β gene from ayu, Plecoglossus altivelis

Characterization of interleukin-1β as a proinflammatory cytokine in grass carp (Ctenopharyngodon idella)

“…This finding is highly consistent with an earlier experimental observation demonstrating that sea bass IL-1b in leucocytes from head kidney, spleen, gill, and liver can be up-regulated by LPS [12]. Moreover, the baseline expression levels of gcIL-1b in various tissues measured in this study are in line with those observed in ayu [20]. However, these expression profiles are not in strict accordance with those reported in other fish [17,18].…”

“…On the other hand, this result also suggests that aspartic acid residues are not completely conserved within the ICE cleavage sites in fish. It should be noted that, as in other fish and mammalian IL1bs [20,44], gcIL-1b also lacks a signal peptide and is a nonclassically secreted protein as predicted by the SecretomeP 2.0 Server online tool. It is presently believed that IL-1b is released from cells by a non-classical secretory pathway and functions as a proinflammatory cytokine [44].…”

“…In another study, the putative mature peptide of yellowfin seabream IL-1b was generated by alignment with human IL-1b, and the recombinant IL-1b was found to induce transcription of endogenous IL-1b in a dose-dependent manner [18]. Recently, an antibody raised against recombinant ayu pro-IL-1b was also found to reduce monocyte/macrophage killing of Listonella anguillarum [20]. In the current study, we generated recombinant grass carp pro-IL-1b carrying a Trx tag, which has been verified to play a significant role in the intestinal inflammatory response.…”

“…To date, IL-1b has been identified in several teleost and cartilaginous fish species, including rainbow trout [9], common carp (Cyprinus carpio) [11], sea bass (Dicentrarchus labrax) [12], Atlantic salmon (Salmo salar) [13], channel catfish (Ictalurus punctatus) [14], Nile tilapia (Oreochromis niloticus) [15], Atlantic cod (Gadus morhua) [16], orange-spotted grouper (Epinephelus coioides) [17], yellowfin seabream (Acanthopagrus latus) [18], roughskin sculpin (Trachidermus fasciatus) [19], ayu (Plecoglossus altivelis) [20], Atlantic bluefin tuna (Thunnus thynnus) [21], and catshark (Scyliorhinus canicula) [22]. Several studies have shown that fish IL-1b proteins are involved in regulating the inflammatory response to bacterial or parasitic infection [23e26].…”

Dual-parallel inhibition of IL-10 and TGF-β1 controls LPS-induced inflammatory response via NF-κB signaling in grass carp monocytes/macrophages

“…For examples, TNF-a functions as an activator and mediator of respiratory activity and phagocytosis in trout and goldfish macrophages [35,36]. Trout IL-1b can increase the phagocytic activity of head kidney leukocytes and ayu IL-1b plays an important role in regulating bacteria killing capability of monocytes/macrophages [37,38]. Carp iNOS has been demonstrated to be responsible for bacteria clearance [20], and recombinant grass carp IL-8 induces neutrophil infiltration and erythrocyte extravasation [39].…”