Molecular Characterization of Ahp2, a Lytic Bacteriophage of Aeromonas hydrophila

Wang, Jianbin; Yu, Mei-Shiuan; Tseng, Tsai-Tien; Lin, Ling-Chun

doi:10.3390/v13030477

Cited by 12 publications

(13 citation statements)

References 60 publications

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“…In general, phages are highly specific, infecting only one bacterial genus or even particular strains [ 224 ]. Most Aeromonas phages showed narrow host specificity, infecting only the original host bacterium [ 51 , 93 , 150 , 214 ] or strains of a given bacterial species [ 91 , 94 , 95 , 149 , 209 , 220 , 230 , 231 ] ( Table S2 ). The majority of marine phages are highly host-specific [ 232 , 233 , 234 ], and about 73% lyse only the original host bacterium [ 234 ].…”

Section: Challenges Associated With the Use Of Phages To Control ...mentioning

confidence: 99%

“…In other studies, A. hydrophila phage adsorption reached over 90% after 40 min [ 95 ], while El- Araby et al (2016) showed a 51% and 66.8% adsorption after 20 and 30 min, respectively [ 93 ]. Phage Ahp2 showed a high adsorption efficacy with 96% of phage particles being adsorbed to A. hydrophila in the first 18 min [ 230 ]. Chen et al (2018) reported that the five isolated phages (AS-szw, AS-yj, AS-zj, AS-sw and AS-gz) showed strong adsorption to A. salmonicida surface and approximately 90% (2.7 × 10 4 PFU/mL out of 3.1 × 10 4 PFU/mL) of phage particles adsorbed to A. salmonicida within the first 5 min [ 217 ].…”

Section: Challenges Associated With the Use Of Phages To Control ...mentioning

confidence: 99%

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Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View

et al. 2022

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Aeromonas species often cause disease in farmed fish and are responsible for causing significant economic losses worldwide. Although vaccination is the ideal method to prevent infectious diseases, there are still very few vaccines commercially available in the aquaculture field. Currently, aquaculture production relies heavily on antibiotics, contributing to the global issue of the emergence of antimicrobial-resistant bacteria and resistance genes. Therefore, it is essential to develop effective alternatives to antibiotics to reduce their use in aquaculture systems. Bacteriophage (or phage) therapy is a promising approach to control pathogenic bacteria in farmed fish that requires a heavy understanding of certain factors such as the selection of phages, the multiplicity of infection that produces the best bacterial inactivation, bacterial resistance, safety, the host’s immune response, administration route, phage stability and influence. This review focuses on the need to advance phage therapy research in aquaculture, its efficiency as an antimicrobial strategy and the critical aspects to successfully apply this therapy to control Aeromonas infection in fish.

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Section: Challenges Associated With the Use Of Phages To Control ...mentioning

confidence: 99%

Section: Challenges Associated With the Use Of Phages To Control ...mentioning

confidence: 99%

Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View

et al. 2022

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“…100 μl of supernatant was removed to calculate the titer of unadsorbed phages. One-step growth and burst-size measurements were performed as previously reported 58 . All experiments were performed at least twice in triplicates.…”

Section: Methodsmentioning

confidence: 99%

“…The host range was determined by spotting 5 µl of phage lysate on the lawns of investigated cultures on TSB3S agar plates, as previously described 58 . A clear spot indicates host sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a Vibrio owensii phage phi50-12

Lin

Tsai

2022

Sci Rep

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Vibrio owensii is a widely distributed marine vibrio species that causes acute hepatopancreatic necrosis in the larvae of Panulirus ornatus and Penaeus vannamei, and is also associated with Montipora white syndrome in corals. We characterized V. owensii GRA50-12 as a potent pathogen using phenotypic, biochemical, and zebrafish models. A virulent phage, vB_VowP_phi50-12 (phi50-12), belonging to the N4-like Podoviridae, was isolated from the same habitat as that of V. owensii GRA50-12 and characterized. This phage possesses a unique sequence with no similar hits in the public databases and has a short latent time (30 min), a large burst size (106 PFU/infected cell), and a wide range of pH and temperature stabilities. Moreover, phi50-12 also demonstrated a strong lysis ability against V. owensii GRA50-12. SDS-PAGE revealed at least nine structural proteins, four of which were confirmed using LC–MS/MS analysis. The size of the phi50-12 genome was 68,059 bp, with 38.5% G + C content. A total of 101 ORFs were annotated, with 17 ORFs having closely related counterparts in the N4-like vibrio phage. Genomic sequencing confirmed the absence of antibiotic resistance genes or virulence factors. Comparative studies have shown that phi50-12 has a unique genomic arrangement, except for the well-conserved core regions of the N4-like phages. Phylogenetic analysis demonstrated that it belonged to a group of smaller genomes of N4-like vibrio phages. The therapeutic effect in the zebrafish model suggests that phi50-12 could be a potential candidate for application in the treatment of V. owensii infection or as a biocontrol agent. However, further research must be carried out to confirm the efficacy of phage50-12.

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“…Aeromonas myophage genomics and biology have been described previously in comprehensive reviews [17,20]. Recently, a number of new Aeromonas phages with podovirus morphotype were described and their genomes appeared in the NCBI GenBank database [19,38,40,[42][43][44]. To evaluate the relations between the studied AerP_220 phage and other Aeromonas phages, vConTACT2 analysis was performed for all Aeromonas phage genomes available in the GenBank database (Table S2, Figure 6).…”

Section: Protein-based Similarity Networkmentioning

confidence: 99%

A Novel Aeromonas popoffii Phage AerP_220 Proposed to Be a Member of a New Tolavirus Genus in the Autographiviridae Family

Morozova

Kozlova

Jdeed

et al. 2022

Viruses

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Aeromonas popoffii is one of the environmental Aeromonas species. A number of factors of virulence have been described for this species and it has been reported as a causative agent of urinary tract infection. The first A. popoffii bacteriophage AerP_220 along with its host strain A. popoffii CEMTC 4062 were isolated from river water. The phage has a podovirus morphotype, shows a narrow host range and is lytic against the host strain. The AerP_220 genome comprises 45,207 bp and does not contain genes responsible for antibiotic resistance and toxin production. Fifty-nine co-directional putative ORFs were found in the AerP_220 genome. Thirty-three ORFs encoded proteins with predicted functions; the products of 26 ORFs were hypothetical proteins. AerP_220 genome analysis revealed that this phage can be considered a novel species within the Autographiviridae family. Comparative genomic and proteomic analysis revealed that AerP_220 along with the Aeromonas phage vB_AspA_Tola (OM913599) are members of a new putative Tolavirus genus in the family Autographiviridae. The Gajwadongvirus and proposed Tolavirus genera along with Pantoea phage Nufs112 and phage Reminis could form a new Tolavirinae subfamily within the Autographiviridae family.

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Molecular Characterization of Ahp2, a Lytic Bacteriophage of Aeromonas hydrophila

Cited by 12 publications

References 60 publications

Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View

Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View

Isolation and characterization of a Vibrio owensii phage phi50-12

A Novel Aeromonas popoffii Phage AerP_220 Proposed to Be a Member of a New Tolavirus Genus in the Autographiviridae Family

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