Molecular characterization of adenovirus from clinical samples through analysis of the hexon and fiber genes

Rosa, Giuseppina La; Iaconelli, Marcello; Pourshaban, M.; Luca, Emilia; Valentini, Piero; Sica, Simona; Manzara, Stefania; Delogu, Giovanni; Muscillo, Michele

doi:10.1099/vir.0.023176-0

Cited by 14 publications

(11 citation statements)

References 54 publications

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“…Concerning type identification, it was well established that sequence analysis of PCR products in the hexon and/or fiber gene is an appropriate method to type HAdVs [Ariga et al, ; La Rosa et al, ] and our results show a high concordance rates between the two genomic regions. On all the sequenced isolates, only one gave different genotype results in the hexon and the fiber gene: sample C125‐TUN13 which was typed as B3 by sequence analysis in the hexon gene and D8 in the fiber gene.…”

Section: Discussionsupporting

confidence: 69%

“…Several studies reported a seasonal increase in the incidence of conjunctivitis cases, especially in spring and summer periods. This can be attributed to the known seasonal high transmission of some aetiological pathogens, or to allergic conjunctivitis which has a relatively higher incidence in spring [Saitoh-Inagawa et al, 1999;Marangon et al, 2007;Matsui et al, 2007;Sambursky et al, 2007;La Rosa et al, 2011;Segal et al, 2014;Erdin et al, 2015].…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection and characterization through analysis of the hexon and fiber genes of Adenoviruses causing conjunctivitis in Tunisia, North Africa

Fedaoui

Ayed²,

Yahia

et al. 2016

Journal of Medical Virology

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Human adenoviruses (HAdVs) are common causes of conjunctivitis. This study describes the epidemiological features and characterizes by phylogenetic analysis HAdVs isolated from patients with conjunctivitis in Tunisia, North Africa. Data on out-patients presenting with conjunctivitis during 2 years (2012-2013) were analyzed. Conjunctival swabs obtained from 240 patients were assessed for the presence of HAdVs by PCR amplification on the fiber and hexon genes. Positive PCR products, together with those of nine viral isolates from previous years, were sequenced and analyzed phylogenetically. Conjunctivitis represented 11.5% of all reasons of consultations with a slight increase between mid-March and mid-June. Sixty-five percent of samples (n = 156) revealed positive by at least one PCR test. PCR amplification in the hexon gene was slightly more sensitive as compared to the fiber gene. Genotyping in the two genomic regions gave concordant results for almost all isolates. HAdV-D8 was the most predominant genotype (87.6%) and was detected continuously from 2000 to 2013. Minor co-circulating genotypes including HAdV-E4, HAdV-B3, HAdV-B55, and HAdV-D37 were identified; most of them were detected by amplification in the hexon gene. In conclusion, this work reports molecular data on adenoviral conjunctivitis from a region where such information is scarce and contributes to a better knowledge of the worldwide distribution of causative genotypes. It revealed a predominance and endemic circulation of HAdV-D8, a genotype that was mainly reported from epidemic keratoconjunctivitis. It shows that PCR amplification in two different genomic regions enhances the sensitivity of HAdV detection in clinical samples and the identification of minor genotypes. J. Med. Virol. 89:304-312, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Molecular detection and characterization through analysis of the hexon and fiber genes of Adenoviruses causing conjunctivitis in Tunisia, North Africa

Fedaoui

Ayed²,

Yahia

et al. 2016

Journal of Medical Virology

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“…Owing to its hypervariable regions, the hexon region is useful for the classification and recognition of individual serotypes [ 6 ]. Indeed, the broad-range primer pair used in this study has been previously found to be capable of amplifying all known HAdVs [ 4 ]. Positive samples were subsequently tested using an assay targeting the fiber region.…”

Section: Discussionmentioning

confidence: 99%

“…Both the hexon and the fiber genes are considered “hot spots” for base mutations and recombination, which are important driving forces for AdV evolution. Therefore, sequencing the hypervariable regions of the hexon gene and the fiber gene is important for the correct identification and designation of adenovirus strains as demonstrated in previous investigations [ 4 , 7 ]. In the present study, 63% of the positive samples detected in the hexon region were also positive using the fiber assay, and BLAST analysis was concordant with the hexon analysis for these sequences.…”

Section: Discussionmentioning

confidence: 99%

“…These primers are able to amplify PCR fragments ranging from 764 to 896 bp in the first PCR, and from 688 to 821 bp in the nested PCR, depending on serotype and species [ 3 ]. For amplification of partial fibre gene sequences, published primers based on the conserved regions of this gene were used [ 4 ]. The list of primers used in the present study is shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

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Genetic Diversity of Human Adenovirus in Children with Acute Gastroenteritis, Albania, 2013–2015

Rosa

Libera

Petricca

et al. 2015

BioMed Research International

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The objectives of the present study were to assess the occurrence of human adenoviruses (HAdVs) in paediatric patients with gastroenteritis in Albania and to characterize HAdV strains. Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene. Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains. Adenovirus DNA was detected in 33/142 samples (23.2%); 14 belonged to species F (13 HAdV-41 and 1 HAdV-40), 13 to species C (1 HAdV-1, 8 HAdV-2, and 4 HAdV-5), 5 to species B (HAdV-3), and 1 to species A (HAdV-12). Rotavirus coinfection was present in 9/33 (27.2%) positive samples. In the remaining 24 positive samples (12 enteric—F species; 12 nonenteric—A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization. This is the first study investigating the presence of human adenoviruses (species A–G) as etiologic agents of viral gastroenteritis in children in Albania.

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Prevalence and quantitation of adenovirus DNA from human tonsil and adenoid tissues

Alkhalaf

Guiver²,

Cooper

2013

Journal of Medical Virology

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In this study, real-time PCR was used to quantify adenovirus DNA in cell suspensions prepared from 106 right and left tonsils and 10 adenoids obtained from 57 patients who underwent routine tonsillectomies and/or adenoidectomies. Eighty-four (72.4%) tonsils and adenoids samples were positive for HAdV by real-time PCR. The viral load ranged from 2.8 × 10(2) to 2.6 × 10(6) copies/10(7) cells and varied up to sixfold between the right and left tonsils. In some cases, only one tonsil was positive and the viral load was lower in older children. Seventy-eight of 84 positive samples could be typed by sequencing of the hexon L1 region. Species C (types 1, 2, and 5) were detected in 84.1% of the patients followed by types 3 and 7 of species B (6.8%), HAdV-E4 (6.8%), and HAdV-F41 (2.3%). In one patient adenovirus C2 was found in the left tonsil and adenovirus C5 in the right tonsil. No DNA methylation was detected in either the E1A promoter or the major late promoter region of adenovirus DNA from six tonsils and adenoids samples and two clinical isolates.

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Molecular characterization of adenovirus from clinical samples through analysis of the hexon and fiber genes

Cited by 14 publications

References 54 publications

Molecular detection and characterization through analysis of the hexon and fiber genes of Adenoviruses causing conjunctivitis in Tunisia, North Africa

Molecular detection and characterization through analysis of the hexon and fiber genes of Adenoviruses causing conjunctivitis in Tunisia, North Africa

Genetic Diversity of Human Adenovirus in Children with Acute Gastroenteritis, Albania, 2013–2015

Prevalence and quantitation of adenovirus DNA from human tonsil and adenoid tissues

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