Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Dufková, Lucie; Kulich, Pavel; Prodělalová, Jana

doi:10.17221/1558-vetmed

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…Second, we did not test the piglet samples for detecting bacterial pathogens (e.g. Escherichia coli ) and other viruses (porcine epidemic diarrhoea virus, porcine norovirus, porcine sapovirus, porcine astrovirus, transmissible gastroenteritis virus) which are also causing diarrhoea in piglets (Chen, Knutson, Rossow, Saif, & Marthaler, ; Dufkova, Kulich, & Prodelalova, ; Kumthip et al, ; Pizzurro et al, ; Shen et al, ). However, our study detected rotavirus among all samples of piglets ( n = 36) and study results suggested rotavirus was the primary cause of diarrhoea in piglets in this study.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of diarrhoea in piglets caused by novel rotavirus genotype G4P[49] in north‐western district of Bangladesh, February 2014

Sarkar

Esona

Gautam³

et al. 2019

Transbound Emerg Dis

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Group A rotavirus (RVA) associated diarrhoea in piglets represents one of the major causes of morbidity and mortality in pig farms worldwide. A diarrhoea outbreak occurred among nomadic piglets in north‐western district of Bangladesh in February 2014. Outbreak investigation was performed to identify the cause, epidemiologic and clinical features of the outbreak. Rectal swabs and clinical information were collected from diarrhoeic piglets (n = 36). Rectal swabs were tested for RVA RNA by real‐time reverse transcription polymerase chain reaction (rRT‐PCR) using NSP3‐specific primers. The G (VP7) and P (VP4) genes were typed by conventional RT‐PCR and sanger sequencing and full genome sequences were determined using next‐generation sequencing. We found the attack rate was 61% (50/82) among piglets in the nomadic pig herd, and the case fatality rate was 20% (10/50) among piglets with diarrhoea. All study piglets cases had watery diarrhoea, lack of appetite or reluctance to move. A novel RVA strain with a new P[49] genotype combined with G4 was identified among all piglets with diarrhoea. The genome constellation of the novel RVA strains was determined to be G4‐P[49]‐I1‐R1‐C1‐M1‐A8‐N1‐T7‐E1‐H1. Genetic analysis shows that the novel G4P[49] strain is similar to Indian and Chinese porcine or porcine‐like G4 human strains and is genetically distant from Bangladeshi human G4 strains. Identification of this novel RVA strain warrants further exploration for disease severity and zoonotic potential.

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Section: Discussionmentioning

confidence: 99%

Outbreak of diarrhoea in piglets caused by novel rotavirus genotype G4P[49] in north‐western district of Bangladesh, February 2014

Sarkar

Esona

Gautam³

et al. 2019

Transbound Emerg Dis

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“…However, the virus cannot be excluded as a causative or contributor agent of diarrhoea because studies have associated the presence of porcine SaV with enteric disease (Kim et al 2006;Martella et al 2008;Zhang et al 2008;Dufkova et al 2011). Porcine SaV has also been reported in mixed infections with porcine norovirus Scheuer et al 2013), Aichivirus C and/or porcine astrovirus (Dufkova et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Porcine SaV belongs to the genogroup GIII and is represented by the prototype Cowden strain . Based on genetic variability of the porcine SaV, the GIII genogroup can be divided into three lineages: 1, 2 and 3 (Collins et al 2009;Reuter et al 2010;Dufkova et al 2011). Novel porcine sapovirus genogroups have been proposed based on the capsid gene (GVI, GVII and GVIII) or the RdRp region (GIX?…”

Section: Introductionmentioning

confidence: 99%

Age distribution of porcine sapovirus asymptomatic infection and molecular evidence of genogroups GIII and GIX? circulation in distinct Brazilian pig production systems

Valente

Alfieri

Barry

et al. 2015

Trop Anim Health Prod

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This study aimed to evaluate the natural infection by SaV in pigs of different categories of production cycle in an important Brazilian pig-producing region. Faecal samples (n = 169) of suckling, post-weaning, finisher and breeder pig categories were analysed. Animals were from five farrow-to-weaning and nine grower-to-finish commercial pig farms. The RT-PCR assay was performed targeting the partial RNA-dependent RNA polymerase (RdRp) gene of porcine SaV genome. The virus was detected in 23.7% (40/169) of faecal samples and in 10/14 (5/5 farrow-to-weaning; 5/9 grower-to-finish) of pig farms evaluated. Porcine SaV was most frequently (p < 0.05) detected in pigs at post-weaning than in grower-to-finish and breeder categories. The phylogenetic analysis revealed that the porcine SaV strains belong to the GIII and GIX? genogroups. This study showed that the porcine SaV GIII genogroup has spread in the pig herds and provides the first evidence of GIX? genogroup circulation in South America.

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“…Human SaV (HuSaV) strains are included within genogroups GI (genotypes GI.1-GI.8), GII (genotypes GII.1-GII.8), GIV (genotype GIV.1), and GV (genotypes GV.1 and GV.2) ( Farkas et al , 2004 ; Katayama et al , 2004 ; Reuter et al , 2010 ; Dufkova et al , 2011 ; Shibata et al , 2015 ; Diez-Valcarce et al , 2018 ; Xue et al , 2019 ). Pigs are infected by genogroups GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI ( Wang et al , 2005 ; Nakamura et al , 2010 ; Reuter et al , 2010 ; Dufkova et al , 2011 ; Kuroda et al , 2017 ; Diez-Valcarce et al , 2018 ) and dogs by genogroup GXIII ( Kuroda et al , 2017 ; Diez-Valcarce et al , 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of norovirus and sapovirus detected in animals and humans in Costa Rica: Zoo-anthropozoonotic potential of human norovirus GII.4

Matamoros¹,

Worsfold²,

Campos³

et al. 2023

Open Vet J

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Background: Noroviruses (NoV) and sapoviruses (SaV) are a major cause of acute viral gastroenteritis in humans worldwide, as well as gastrointestinal infection in animals. However, it has not been determined whether these viruses are zoonotic pathogens. Aim: In this study, we investigated the presence of NoV and SaV in stool samples from dogs, pigs, cows, and humans to determine some aspects of the molecular epidemiology and the genetic relationship of several strains present in these species. Methods: Polymerase chain reaction and sequencing of NoV and SaV strains present in stool samples from humans and dogs with diarrhea, pigs and cattle with and without diarrhea were carried out during fragmented periods from 2002 to 2012. Results: Of all samples analyzed, 11.6% (123/1,061) of the samples were positive for NoV and 0.88% (9/1,023) were positive for SaV. The phylogenetic analysis confirmed 16 human strains of NoV (HuNoV) belonging to HuNoV G?/GII.P2 (1), GII.4/GII.P4 (5), G?/GII.P4 (9) and GII.6/GII.P6 (1) and allowed us to verify and assign three strains of human SaV to genotypes GI.2 (1) and GII.5 (2). In dogs, eight strains of NoV [HuNoV G?/GII.P4 (4) and canine G?/GVI.P1 (4)] and two strains of canine SaV were determined. In pigs, six strains were assigned to HuNoV G?/GII.P4 and four strains to porcine SaV were assigned to genogroup GIII (2), GVIII (1), and GXI (1). In bovines, five strains were characterized as HuNoV G?/GII.P4. Conclusions: This study showed that NoV and SaV prototype strains have been present in humans and dogs in Costa Rica. Additionally, it revealed that the zoonotic potential of SaV is very limited, while the zoonotic implications for HuNoV GII.4 are stronger due to the simultaneous circulation of strains related to HuNoV GII.4 in four species, which suggests a zoo-anthropozoonosis.

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Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Cited by 6 publications

References 27 publications

Outbreak of diarrhoea in piglets caused by novel rotavirus genotype G4P[49] in north‐western district of Bangladesh, February 2014

Outbreak of diarrhoea in piglets caused by novel rotavirus genotype G4P[49] in north‐western district of Bangladesh, February 2014

Age distribution of porcine sapovirus asymptomatic infection and molecular evidence of genogroups GIII and GIX? circulation in distinct Brazilian pig production systems

Molecular characterization of norovirus and sapovirus detected in animals and humans in Costa Rica: Zoo-anthropozoonotic potential of human norovirus GII.4

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