Molecular characterization of a p38 mitogen-activated protein kinase gene from Scylla paramamosain and its expression profiles during pathogenic challenge

Yu, Zehui; Geng, Yi; Huang, Anming; Wang, Kaiyu; Huang, Xiaoli; Chen, Defang; Ou, Yangping; Wang, Jun

doi:10.1016/j.jip.2017.01.001

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…The phylogenetic analysis provided evidences that Bmp38 and BmS6K homologous are highly conserved in vertebrates and invertebrates. This phylogenetic analysis is consistent with the previous studies (Lin et al 2017, Yu et al 2017c).…”

Section: Discussionsupporting

confidence: 93%

“…Similar evidence from different organisms showed p38 MAPK signaling pathway mRNA expression was increased after pathogen infection (He et al 2013, 2018; Yan et al 2013; Zhu et al 2014). A p38 protein from Scylla paramamosain showed that it is involved in immune response against bacteria and virus (Yu et al 2017c). These findings suggest that Bmp38 may be involved in immune response against BmNPV.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection

Muhammad

Toufeeq

et al. 2019

Journal of Insect Science

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Proteins p38 map kinase and ribosomal S6 kinase (S6K) as members of mitogen-activated protein kinases (MAPKs) play important roles against pathogens. In this study, Bmp38 and BmS6K were identified as differentially expressed proteins from iTRAQ database. Bmp38 and BmS6K were expressed, and recombinant proteins were purified. The bioinformatics analysis showed that both proteins have serine/threonine-protein kinases, catalytic domain (S_ TKc) with 360 and 753 amino acids, respectively. The real-time quantitative polymerase chain reaction (RT-qPCR) results suggest that Bmp38 and BmS6K had high expression in the midgut and hemolymph. The comparative expression level of Bmp38 and BmS6K in BC9 was upregulated than in P50 in the midgut after Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Western bolt results showed a positive correlation between RT-qPCR and iTRAQ data for Bmp38, but BmS6K data showed partial correlation with iTRAQ. Injection of anti-Bmp38 and anti-BmS6K serum suggested that Bmp38 may be involved against BmNPV infection, whereas BmS6K may require phosphorylation modification to inhibit BmNPV infection. Taken together, our results suggest that Bmp38 and BmS6k might play an important role in innate immunity of silkworm against BmNPV.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection

Muhammad

Toufeeq

et al. 2019

Journal of Insect Science

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“…In this study, we cloned the full-length cDNA of P. trituberculatus PtP38. Multiple sequence alignment showed that PtP38 shares the highest identity with Scylla Paramamosain (Yu et al, 2017), followed by Eriocheir sinensis (Zhu et al, 2015). The PtP38 gene is expressed in the heart, muscle, hepatopancreas, gills, stomach, eyestalk, mucosa, testis, and ovaries.…”

Section: Discussionmentioning

confidence: 99%

“…The second group was injected with 0.2 mL of PBS per 100 g of crab body weight (control group). The third group was injected with 0.1 mL of SB203580 per 100 g of crab body weight 1 h ahead and then with 0.2 mL of LPS per 100 g of crab body weight as the SB203580 + LPS group (Yu et al, 2017). The culture conditions remained the same.…”

Section: Laboratory Animals Stimulation and Samplingmentioning

confidence: 99%

“…After the in vivo infection with Vibrio splendidus, P38 mRNA expression in sea cucumber Apostichopus japonicus was significantly up-regulated (Zhan et al, 2018). After stimulation with Staphylococcus aureus, Vibrio harveyi, and White Spot Syndrome virus, the expression levels of Scylla paramamosain and Fenneropenaeus chinensis P38 MAPK were up-regulated (Yu et al, 2017). Additionally, knockdown of P38 by RNA interference (RNAi) resulted in a reduced expression of MKK3 (one of the P38 MAPKK) and ATF-2 (one of the P38 MAPK effector proteins) in Fenneropenaeus chinensis (He et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

Wei

Zhu

et al. 2021

Front. Mar. Sci.

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The P38 mitogen-activated protein kinase (MAPK) signal transduction pathway is widespread in organisms and plays important roles in immune activities. The infection mechanism of environmental gram-negative bacteria on crustaceans is an important scientific problem. In this study, the cDNA full-length sequence of Portunus trituberculatus P38 (PtP38) was cloned and its structure was analyzed by bioinformatics methods. To study the function of the PtP38 gene after a Gram-negative bacterial infection, we injected P. trituberculatus with LPS to activate the immune response instead of directly infecting with Gram-negative bacteria. With LPS stimulation, the expression of the PtP38 gene in different tissues increased significantly. At the same time, the expression of immune-related genes (ALF and crustin) in the hepatopancreas, activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), and inducible nitric oxide synthase (iNOS) enzymes], and expression of apoptosis-related genes (caspase2 and caspase3) were increased significantly. To further conform the function of PtP38 in the immune response, we injected P. trituberculatus with P38 inhibitor and subsequently injected with LPS. The results showed that the expression of immune-related genes was inhibited, the activity of antioxidant enzymes was decreased, and the expression of apoptosis-related genes were inhibited. Thus, we speculated that PtP38 may increase the immune ability by improving the expression of antimicrobial peptides, increasing the activity of oxidative stress-related enzymes, and promoting cell apoptosis in infected P. trituberculatus. This study also laid the foundation for further study of the P38 MAPK signaling pathway and immune mechanism of P. trituberculatus.

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Identification and functional analysis of inhibitor of NF-κB kinase (IKK) from Scylla paramamosain: The first evidence of three IKKs in crab species and their expression profiles under biotic and abiotic stresses

Jiang

et al. 2018

Developmental & Comparative Immunology

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Molecular characterization of a p38 mitogen-activated protein kinase gene from Scylla paramamosain and its expression profiles during pathogenic challenge

Cited by 12 publications

References 33 publications

Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection

Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection

PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

Identification and functional analysis of inhibitor of NF-κB kinase (IKK) from Scylla paramamosain: The first evidence of three IKKs in crab species and their expression profiles under biotic and abiotic stresses

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