Molecular characterization of a novel double-stranded RNA virus infecting the entomopathogenic fungus Metarhizium brunneum

Wang, Ping; Yang, Guogen; Shi, Najie; Huang, Bo

doi:10.1007/s00203-022-03224-x

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…Although a high prevalence of viral sequences has been found in Metarhizium species, only three viral genomes are available to date. Notably, all available genomes belong to Partiviridae (dsRNA) family [12][13][14].…”

Section: Full Textmentioning

confidence: 99%

Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae

et al. 2023

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Section: Full Textmentioning

confidence: 99%

Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae

et al. 2023

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“…mycoviruses have been reported and characterized by the Research Center for Entomogenous Fungi of Anhui Agricultural University (RCEF). These include Metarhizium brunneum partitivirus 1 (MbPV1) and 2 (MbPV2), classified respectively to the genera Epsilonpartitivirus and Gammapartitivirus in the family Partitiviridae [21,22], and the unassigned Metarhizium brunneum bipartite mycovirus 1 (MbBV1) [23]. There is evidence that Metarhizium spp.…”

Section: Introductionmentioning

confidence: 99%

A novel partitivirus orchestrates conidiation, stress response, pathogenicity, and secondary metabolism of the entomopathogenic fungus Metarhizium majus

et al. 2023

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Mycoviruses are widely present in all major groups of fungi but those in entomopathogenic Metarhizium spp. remain understudied. In this investigation, a novel double-stranded (ds) RNA virus is isolated from Metarhizium majus and named Metarhizium majus partitivirus 1 (MmPV1). The complete genome sequence of MmPV1 comprises two monocistronic dsRNA segments (dsRNA 1 and dsRNA 2), which encode an RNA-dependent RNA polymerase (RdRp) and a capsid protein (CP), respectively. MmPV1 is classified as a new member of the genus Gammapartitivirus in the family Partitiviridae based on phylogenetic analysis. As compared to an MmPV1-free strain, two isogenic MmPV1-infected single-spore isolates were compromised in terms of conidiation, and tolerance to heat shock and UV-B irradiation, while these phenotypes were accompanied by transcriptional suppression of multiple genes involved in conidiation, heat shock response and DNA damage repair. MmPV1 attenuated fungal virulence since infection resulted in reduced conidiation, hydrophobicity, adhesion, and cuticular penetration. Additionally, secondary metabolites were significantly altered by MmPV1 infection, including reduced production of triterpenoids, and metarhizins A and B, and increased production of nitrogen and phosphorus compounds. However, expression of individual MmPV1 proteins in M. majus had no impact on the host phenotype, suggesting insubstantive links between defective phenotypes and a single viral protein. These findings indicate that MmPV1 infection decreases M. majus fitness to its environment and its insect-pathogenic lifestyle and environment through the orchestration of the host conidiation, stress tolerance, pathogenicity, and secondary metabolism.

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“…Metarhizium, like many other fungi, is often associated with the presence of virus. Four dsRNA viruses have been identified and classified from M. brunneum, including two partitiviruses (MbPV1 and MbPV2) (Wang et al, 2020, an unassigned mycovirus (MbBV1) (Wang et al, 2022), and a polymycovirus (MbPmV1) (He et al, 2023). The effects of mycovirus infection on insect pathogenic fungi have been extensively studied, with mycovirus infection causing a significant decrease in host mycelial growth, conidiation, and virulence in M. anisopliae (Melzer and Bidochka, 1998;Gimenez-Pecci et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Infection with a novel polymycovirus enhances growth, conidiation and sensitivity to UV-B irradiation of the entomopathogenic fungus Metarhizium anisopliae

Wang

Yang

et al. 2023

Front. Microbiol.

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Metarhizium anisopliae is a well-studied entomopathogenic fungus that is widely used in biological control programs. The presence of polymycoviruses in this fungus is common, but their effects on fungal development and stress tolerance are not well understood. In this study, we report the discovery of a novel double-stranded RNA virus, named Metarhizium anisopliae polymycovirus 1 (MaPmV1), which comprises four dsRNAs ranging from 2.4 to 1.4 kbp in length. Phylogenetic analysis revealed that MaPmV1 belongs to the Polymycoviridae family. Biological comparison between MaPmV1-infected (Vi) and -free (Vf) isogenic lines showed that MaPmV1 remarkably enhances the growth rate and conidiation of the host fungus. The upregulation of growth- and conidiation-related genes in Vi strains supports this finding. In addition, MaPmV1 increases the sensitivity of the host to UV-B irradiation, which is evidenced by the downregulation of DNA damage repair genes in Vi strains. However, MaPmV1 does not appear to have any significant impact on the virulence of M. anisopliae. Furthermore, overexpression of individual viral proteins in M. anisopliae did not result in any significant phenotypic alterations, indicating that MaPmV1-mediated changes are not related to a single viral protein. Overall, our findings suggest that mycoviruses can be exploited to enhance fungal development in entomopathogenic fungi, which may lead to improved conidium production on a large scale.

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Molecular characterization of a novel double-stranded RNA virus infecting the entomopathogenic fungus Metarhizium brunneum

Cited by 7 publications

References 25 publications

Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae

Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae

A novel partitivirus orchestrates conidiation, stress response, pathogenicity, and secondary metabolism of the entomopathogenic fungus Metarhizium majus

Infection with a novel polymycovirus enhances growth, conidiation and sensitivity to UV-B irradiation of the entomopathogenic fungus Metarhizium anisopliae

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