Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae

Camargo, Matheus da Silva; Geremia, Felipe; Sbaraini, Nicolau; Staats, Charley Christian; Filho, Marcio Silva; Schrank, Augusto

doi:10.1007/s00705-023-05716-7

Cited by 1 publication

(2 citation statements)

References 26 publications

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“…The complete genome of UvRV16 is 5164 bp in length containing two open reading frames (ORFs), ORF1 and ORF2. The tetranucleotide sequence (AUGA) observed in the overlap of the two ORFs, which is typical of members of the genus Victorivirus in the family Totiviridae , is the ORF2 start codon (AUG) and the ORF1 termination codon (UGA) (King et al 2012 ; da Silva Camargo et al 2023 ). The ORF1 (2259 bp) encodes a capsid protein (CP) with 752 amino acids (aa) that share 44.94% identity with the CP of Helminthosporium victoriae virus 190S (HvV190S, accession number O57043.2).…”

Section: Resultsmentioning

confidence: 99%

“…Phylogenetic tree was constructed by aligning the RdRP amino acid sequences of UvRV16 with five different viral genera ( Victorivirus , Leishmaniavirus , Trichomonasvirus , Totivirus and Giardiavirus ) and unclassified viruses in the family Totiviridae (Fig. 3 a) (King et al 2012 ; da Silva Camargo et al 2023 ). The results indicated that UvRV16 formed an independent branch in the genus Victorivirus (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Co-infection of Four Novel Mycoviruses from Three Lineages Confers Hypovirulence on Phytopathogenic Fungus Ustilaginoidea virens

Fan,

Zhao,

Tang

et al. 2024

Rice

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Rice false smut caused by Ustilaginoidea virens has become one of the most important diseases of rice. Mycoviruses are viruses that can infect fungi with the potential to control fungal diseases. However, little is known about the biocontrol role of hypoviruses in U. virens. In this study, we revealed that the hypovirulence-associated U. virens strain Uv325 was co-infected by four novel mycoviruses from three lineages, designated Ustilaginoidea virens RNA virus 16 (UvRV16), Ustilaginoidea virens botourmiavirus virus 8 (UvBV8), Ustilaginoidea virens botourmiavirus virus 9 (UvBV9), and Ustilaginoidea virens narnavirus virus 13 (UvNV13), respectively. The U. virens strain co-infected by four mycoviruses showed slower growth rates, reduced conidial yield, and attenuated pigmentation. We demonstrated that UvRV16 was not only the major factor responsible for the hypovirulent phenotype in U. vriens, but also able to prevent U. virens to accumulate more mycotoxin, thereby weakening the inhibitory effects on rice seed germination and seedling growth. Additionally, we indicated that UvRV16 can disrupt the antiviral response of U. virens by suppressing the transcriptional expression of multiple genes involved in autophagy and RNA silencing. In conclusion, our study provided new insights into the biological control of rice false smut.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%