Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle

Suganuma, Keisuke; Kondoh, Daisuke; Sivakumar, Thillaiampalam; Mizushima, Daiki; Elata, Afraa; Thekisoe, Oriel; Yokoyama, Naoki; Inoue, Noboru

doi:10.1007/s00436-019-06313-x

Cited by 12 publications

(20 citation statements)

References 34 publications

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“…Confirming previous studies, ITS1 rDNA sequences of Megatrypanum trypanosomes were distributed in the TthI and TthII lineages ( Fig. 6) regardless of originating from bovids or cervids; thus, corroborating data from this (Figs 3-5), and previous studies [12,15,24,25,53]. Our analysis supported 6 genotypes of TthI (IA-IF), and 9 of TthII (IIA-II I) ( Fig.…”

Section: Small Polymorphism Between Its1 Rdna Sequences Of T Perronesupporting

confidence: 91%

“…Trypanosome cross-infections of cervids and bovids have not been confirmed experimentally or by molecular epidemiology [8,12,15,21,24,25,33,53,59]. In Venezuela, we found WTD infected with T. perronei sp.…”

Section: Host-parasite-vector Relationships and Evolutionary History mentioning

confidence: 73%

“…Although useful for assessment of genetic diversity [42,[50][51][52], the small polymorphism detected in short CATL sequences generated by TthCATL-PCR may be insufficient to discriminate reliable lineages and, specially, genotypes. Therefore, the description of novel species and intra-specific genotypes must be supported by additional markers including conserved (SSU rRNA and gGAPDH), and polymorphic (ITS rDNA and SL rRNA) sequences [15,25,42,43,50,53]. Supplementary Table S2).…”

Section: Resultsmentioning

confidence: 99%

“…Japanese sika deer were found infected exclusively with the T. sp. TSD1, whereas sympatric cattle were infected with T. theileri of both TthI and TthII lineages [53].…”

Section: Host-parasite-vector Relationships and Evolutionary History mentioning

confidence: 99%

“…All these findings, coupled with data herein reported, provide strong evidence that Megatrypanum trypanosomes exhibit a narrow host range or even host specificity. Each trypanosome species/genotypes were found in a single host species or in closely phylogenetically related hosts, those found in cervids were never detected in bovids, although one host species can harbour trypanosomes of more than one species or genotype [12,[23][24][25]53]. To date, reports of elk and WTD sharing trypanosome genotype [12] relied merely on DNA detection, and genuine infections remain to be demonstrated.…”

Section: Host-parasite-vector Relationships and Evolutionary History mentioning

confidence: 99%

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Pan-American Trypanosoma (Megatrypanum) perronei sp. n. in white-tailed deer (Odocoileus virginianus) and its deer ked Lipoptena mazamae: morphological, developmental and phylogeographical characterisation

García

Blanco

Rodrigues

et al. 2020

Preprint

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Background The subgenus Megatrypanum comprises trypanosomes of cervids and bovids from around the world. Here, Odocoileus virginianus (white-tailed deer = WTD) and its ectoparasite, the deer ked Lipoptena mazamae (hippoboscid fly), were surveyed for trypanosomes in Venezuela. Results Haemoculturing unveiled 20% infected WTD, while 47% (7/15) of blood samples and 38% (11/29) of ked guts tested positive for the Megatrypanum- specific TthCATL-PCR. CATL and SSU rRNA sequences uncovered a single species of trypanosome. Phylogeny based on SSU rRNA and gGAPDH sequences tightly cluster WTD trypanosomes from Venezuela and the USA, which were strongly supported as geographical variants of the herein described Trypanosoma ( Megatrypanum ) perronei sp. n. In our analyses, T. perronei was closest to T . sp. D30 of fallow deer (Germany), both nested into TthII alongside other trypanosomes of cervids (North American elks and European fallow, red and sika deer), and bovids (cattle, antelopes and sheep). Insights into T. perronei life cycle were obtained from early haemocultures of deer blood and co-culture with mammalian and insect cells showing flagellates resembling Megatrypanum trypanosomes previously reported in deer blood, and deer ked guts. For the first time, a trypanosome from a cervid was cultured and phylogenetically and morphologically (light and electron microscopy) characterised. Conclusions In the analyses based on SSU rRNA, gGAPDH, CATL and ITS rDNA sequences, neither cervids nor bovids trypanosomes were monophyletic but intertwined within TthI and TthII major phylogenetic lineages. One host species can harbour more than one species/genotype of trypanosomes, but each trypanosome species/genotype was found in a single host species or in phylogenetically related hosts. Molecular evidence that L. mazamae may transmit T. perronei suggests important evolutionary constraints making tight the tripartite T. perronei –WTD–deer ked association. In a plausible evolutionary scenario, T. perronei entered South America with North American WTD at the Pliocene–Pleistocene boundary following the closure of the Panama Isthmus.

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Section: Small Polymorphism Between Its1 Rdna Sequences Of T Perronesupporting

confidence: 91%

Section: Host-parasite-vector Relationships and Evolutionary History mentioning

confidence: 73%

Section: Resultsmentioning

confidence: 99%

“…Japanese sika deer were found infected exclusively with the T. sp. TSD1, whereas sympatric cattle were infected with T. theileri of both TthI and TthII lineages [53].…”

Section: Host-parasite-vector Relationships and Evolutionary History mentioning

confidence: 99%

Section: Host-parasite-vector Relationships and Evolutionary History mentioning

confidence: 99%

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Pan-American Trypanosoma (Megatrypanum) perronei sp. n. in white-tailed deer (Odocoileus virginianus) and its deer ked Lipoptena mazamae: morphological, developmental and phylogeographical characterisation

García

Blanco

Rodrigues

et al. 2020

Preprint

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Molecular Detection of Animal Trypanosomes in Different Animal Species in the Visayas Region of the Philippines

et al. 2023

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First Report of Trypanosoma theileri in Equine Host and Tabanus sp. in Malaysia

Peng

Rajdi

Mohamad

et al. 2022

Journal of Equine Veterinary Science

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Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle

Cited by 12 publications

References 34 publications

Pan-American Trypanosoma (Megatrypanum) perronei sp. n. in white-tailed deer (Odocoileus virginianus) and its deer ked Lipoptena mazamae: morphological, developmental and phylogeographical characterisation

Pan-American Trypanosoma (Megatrypanum) perronei sp. n. in white-tailed deer (Odocoileus virginianus) and its deer ked Lipoptena mazamae: morphological, developmental and phylogeographical characterisation

Molecular Detection of Animal Trypanosomes in Different Animal Species in the Visayas Region of the Philippines

First Report of Trypanosoma theileri in Equine Host and Tabanus sp. in Malaysia

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