Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein

Two duplicated chicken-type lysozyme genes in disc abalone Haliotis discus discus: Molecular aspects in relevance to structure, genomic organization, mRNA expression and bacteriolytic function

“…Lysozymes are one of the vital humoral elements which play important roles against pathogenic bacteria as antibacterial proteins. With the documentation of a LysC in Ezo abalone (HdLysC) by Ding et al, mollusc became the first invertebrate phylum in which three major distinct lysozymes (c-, g-and itypes) coexist [1]. In the study presented herein, we describe two gastropod LysC variants isolated from disc abalone H. discus discus at structural, transcriptional and functional levels.…”

“…To date, nearly 16 LysIs and 6 LysGs have been described from different molluscan species [11e16]. It was believed that LysC distribution in invertebrates is limited to arthropods and cephalochordates until Ding et al reported the first molluscan LysC homolog from Ezo abalone recently [1].…”

Identification and expression analysis of three c-type lysozymes in Oreochromis aureus

“…In addition, four disulfide bonds contribute to the exudation of lysozyme through making the lysozyme molecular into an ellipse. Glu 35 and Asp 52 can catalyze the hydrolysis of b-1, 4-glycosidic bond through synergy [23]. In the phylogenetic tree constructed based on lysozyme amino acid sequences, three lysozymes were firstly grouped to those of higher teleosteans, Pleuronectiformes and Tetraodontiformes fishes, and clustered to those of lower teleosteans, cypriniformes fishes at last (Fig.…”

“…Recombinant c-type lysozyme from several shrimp had lytic activities against Vibrio Species [7,32e34]. Recombinant c-type lysozyme from abalone (Haliotis discus hannai Ino) showed bacteriolytic activity against both Gram-positive and Gram-negative bacteria of the Vibrio species tested [35]. Recombinant c-type lysozyme of Japanese flounder produced in a baculovirus expression system showed lytic activity five times higher than that of HEW lysozyme [36].…”

“…ClustalW analyses showed that all three genes possessed two conserved catalytic residues in mature peptide, Glu 35 and Asp 52 , and eight structural cysteine residues which were highly conserved across species of C-type lysozymes (Fig. 1).…”