Molecular characterization by MLVA of Coxiella burnetii strains infecting dairy cows and goats of north-eastern Italy

Ceglie, Letizia; Guerrini, Eulalia; Rampazzo, E.; Barberio, A.; Tilburg, Jeroen J. H. C.; Hagen, Ferry; Lucchese, Laura; Zuliani, Federica; Marangon, Stefano; Natale, Alda

doi:10.1016/j.micinf.2015.09.029

Cited by 19 publications

(16 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, five complete MLVA profiles were gained, all of which were different from the previously reported genotypes, including those recently found in Italy [ 35 ], while 15 panels were incomplete. Despite this, MLVA enabled 10 genotypes to be identified, instead of the 5 obtained by MST.…”

Section: Discussioncontrasting

confidence: 74%

Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

et al. 2018

View full text Add to dashboard Cite

BackgroundAs the epidemiology of human Q Fever generally reflects the spread of Coxiella burnetii in ruminant livestock, molecular characterization of strains is essential to prevent human outbreaks. In this study we report the genetic diversity of C. burnetii in central Italy accomplished by MST and MLVA-6 on biological samples from 20 goat, sheep and cow farms.ResultsFive MST and ten MLVA profiles emerged from the analysis establishing a part of C. burnetii strain world atlas. In particular, ST32 occurred on 12 farms (60%), prevalently in goat specimens, while ST12 (25%) was detected on 4 sheep and 1 goat samples. ST8 and a variant of this genotype were described on 2 different sheep farms, whereas ST55 was observed on a goat farm. Five complete MLVA profiles different from any other published genotypes were described in this study in addition to 15 MLVA incomplete panels. Despite this, polymorphic markers Ms23, Ms24 and Ms33 enabled the identification of samples sharing the same MST profile.ConclusionsIntegration of such data in international databases can be of further help in the attempt of building a global phylogeny and epidemiology of Q fever in animals, with a “One Health” perspective.

show abstract

Section: Discussioncontrasting

confidence: 74%

Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

et al. 2018

View full text Add to dashboard Cite

show abstract

“…All but genotype NM were clustered together and interconnected by repeated number changes in one of the tested loci. Figs 3 and 4 present the relationship between all MLVA genotypes identified in this study, published in the previously described database and publications [2, 8, 24] regarding the animal host species and geographical distribution, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Analysis revealed that the majority of MLVA genotypes circulating in the cattle population in Poland show high genetic similarity and are interconnected by repeated number changes in one of the tested loci, except genotype NM whose allelic profile is different from the others in 5 out of 6 analyzed loci. Many researchers suggest that genotypes differing in the number of short tandem repeats might be microvariants of one genotype [24, 36]. Clustering of the MLVA genotypes using the minimum spanning tree method (Figs 3 and 4) showed high genetic similarity of genotypes identified in cattle from many countries, which may indicate a clonal spread of host-adapted C .…”

Section: Discussionmentioning

confidence: 97%

“…Clustering of obtained MLVA profiles was performed with Bionumerics v.7.6 software (Applied Maths, USA). Minimum spanning trees were generated to show the relationships between MLVA genotypes obtained in this study, the genotypes from the database [23] and those described by González-Barrio et al [2], Piñero et al [8] and Ceglie et al [24]. In total, 459 samples (from cattle n = 202, humans n = 139, goats n = 53, sheep n = 36, ticks n = 11, European rabbits n = 10, red deer n = 6, rodents n = 1 and antelope n = 1) including six sequenced reference strains (Nine Mile RSA 493, Dugway 5J108-111, CbuG_Q212, CbuK_Q154, Cb175_Guyana, and Henzerling RSA 331) were used for comparison.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Shedding and genetic diversity of Coxiella burnetii in Polish dairy cattle

et al. 2019

View full text Add to dashboard Cite

Q fever is a worldwide zoonotic disease reported in humans and many animal species including cattle. The aims of this study were to evaluate the prevalence of Coxiella (C.) burnetii shedding in Polish dairy cattle herds and to identify the pathogen’s genotypes and sequence types (STs) using multiple-locus variable number tandem repeat analysis (MLVA) and multispacer sequence typing (MST) methods. The presence of C. burnetii DNA was detected using a commercial real-time PCR kit, targeting the IS1111 element. Overall, 1,439 samples from 279 herds were tested including: 897 individual milk specimens, 101 bulk tank milk samples, 409 genital tract swabs and 32 placentas. Furthermore, 30 consumer milk samples, including 10 from vending machines and 77 dairy products were also analyzed. C. burnetii shedding was confirmed in 31.54% of tested cattle herds as well as in 69.16% of consumer milk and dairy products. Among real-time PCR–positive samples, 49 specimens obtained from 49 cattle herds and 8 samples of purchased dairy products were selected for genotyping. Overall, five previously known MLVA genotypes (I, J, BG, BE, and NM) and three new ones (proposed as PL1, PL2, and PL3) were identified. Two MST sequence types were recorded: ST16 and a novel sequence (ST61). The new genotypes and sequence types need further research particularly into their pathogenicity to humans.

show abstract

“…C. burnetii was sometimes found in other domestic animals such as poultry, cats, dogs, rabbits, and pigeons (182)(183)(184)(185)(186). Different C. burnetii genotypes circulate in wildlife including clones that are more likely to cross species barrier for infection of livestock and humans (187)(188)(189)(190).…”

Section: The Main Known Reservoirs Of Coxiella Burnetii: Cattle Sheementioning

confidence: 99%

Coxiella burnetii in Dromedary Camels (Camelus dromedarius): A Possible Threat for Humans and Livestock in North Africa and the Near and Middle East?

et al. 2020

View full text Add to dashboard Cite

The "One Health" concept recognizes that human health is connected to animal health and to the ecosystems. Coxiella burnetii-induced human Q fever is one of the most widespread neglected zoonosis. The main animal reservoirs responsible for C. burnetii transmission to humans are domesticated ruminants, primarily goats, sheep, and cattle. Although studies are still too sparse to draw definitive conclusions, the most recent C. burnetii serosurvey studies conducted in herds and farms in Africa, North Africa, Arabian Peninsula, and Asia highlighted that seroprevalence was strikingly higher in dromedary camels (Camelus dromedarius) than in other ruminants. The C. burnetii seroprevalence in camel herds can reach more than 60% in Egypt, Saudi Arabia, and Sudan, and 70 to 80% in Algeria and Chad, respectively. The highest seroprevalence was in female camels with a previous history of abortion. Moreover, C. burnetii infection was reported in ticks of the Hyalomma dromedarii and Hyalomma impeltatum species collected on camels. Even if dromedary camels represent <3% of the domesticated ruminants in the countries of the Mediterranean basin Southern coast, these animals play a major socioeconomic role for millions of people who live in the arid zones of Africa, Middle East, and Asia. In Chad and Somalia, camels account for about 7 and 21% of domesticated ruminants, respectively. To meet the growing consumers demand of camel meat and milk (>5 million tons/year of both raw and pasteurized milk according to the Food and Agriculture Organization) sustained by a rapid increase of population (growth rate: 2.26-3.76 per year in North Africa), dromedary camel breeding tends to increase from the Maghreb to the Arabic countries. Because of possible long-term persistence of C. burnetii in camel hump adipocytes, this pathogen could represent a threat for herds and breeding farms and ultimately for public health. Because this review highlights a hyperendemia of C. burnetii in dromedary camels, a proper screening of herds and breeding farms for C. burnetii is urgently needed in countries where camel breeding is on the rise. Moreover, the risk of C. burnetii transmission from camel to human should be further evaluated.

show abstract

Molecular characterization by MLVA of Coxiella burnetii strains infecting dairy cows and goats of north-eastern Italy

Cited by 19 publications

References 12 publications

Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

Shedding and genetic diversity of Coxiella burnetii in Polish dairy cattle

Coxiella burnetii in Dromedary Camels (Camelus dromedarius): A Possible Threat for Humans and Livestock in North Africa and the Near and Middle East?

Contact Info

Product

Resources

About