Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

Domenico, Marco Di; Curini, Valentina; Lollo, Valeria Di; Massimini, M.; Gialleonardo, Luigina Di; Franco, Alessia; Caprioli, Andrea; Battisti, Antonio; Cammà, Cesare

doi:10.1186/s12917-018-1499-8

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…Previously the ST20 genotype had also been identified in cattle in Hungary (Sulyok et al, 2014). Strains belonging to the ST23 group have been reported in ticks and humans in Slovakia (Di Domenico et al, 2018), but this is the first description of ST61 in cattle in Hungary and Slovakia. This sequence type has been recently described from cattle in Brazil, Argentina, and Poland (Mioni et al, 2019;Szyma nska-Czerwi nska et al, 2019).…”

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confidence: 91%

Prevalence of Coxiella burnetii in bovine placentas in Hungary and Slovakia: Detection of a novel sequence type – Short communication

2021

AVet

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Cotyledons (n = 167) from 30 Hungarian and 5 Slovakian dairy cattle herds were analysed for Coxiella burnetii by real-time PCR targeting the IS1111 gene. Eighty (88.9%) out of the 90 cotyledons from retained placentas and 31 (40.3%) out of the 77 cotyledons from normally separated placentas tested positive. Seventeen out of the 80 positive samples (21.3%) originating from retained placentas were found to be highly loaded with C. burnetii with a cycle threshold (Ct) value lower than or equal to 27.08, ranging between 11.92 and 27.08. The rest of the positive samples from retained fetal membranes and from normally separated placentas were moderately loaded with C. burnetii DNA. Five out of the ten samples showing the strongest positivity (Ct 11.92–18.28) from retained placentas were genotyped by multispacer sequence typing based on ten loci, which revealed sequence type (ST) 61, a type that had not been detected in Hungary and Slovakia previously. Retained placenta was more likely in cows with C. burnetii PCR-positive cotyledons (odds ratio: 12.61, P = 0.0023). The high C. burnetii DNA load found in retained fetal membranes may be a potential risk factor for human infection and may also be associated with the retention of fetal membranes.

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Prevalence of Coxiella burnetii in bovine placentas in Hungary and Slovakia: Detection of a novel sequence type – Short communication

2021

AVet

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“…The MLVA/VNTR uses up to 17 different genomic target regions for the differentiation of C. burnetii strains. Several genotypes could be characterized within the ruminant population across Europe [28][29][30][31]. Nevertheless, using different markers and a variable number of them hamper the comparison.…”

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confidence: 99%

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

et al. 2020

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The main reservoir of Coxiella (C.) burnetii are ruminants. They shed the pathogen through birth products, vaginal mucus, faeces and milk. A direct comparison of C. burnetii excretions between naturally infected sheep and goats was performed on the same farm to investigate species-specific differences. The animals were vaccinated with an inactivated C. burnetii phase I vaccine at the beginning of the study period for public health reasons. Vaginal and rectal swabs along with milk specimens were taken monthly during the lambing period and once again at the next lambing season. To estimate the environmental contamination of the animals’ housings, nasal swabs from every animal were taken simultaneously. Moreover, dust samples from the windowsills and straw beddings were collected. All samples were examined by qPCR targeting the IS1111 gene and the MLVA/VNTR typing method was performed. Whole genome sequencing was applied to determine the number of IS1111 copies followed by a calculation of C. burnetii genome equivalents of each sample. The cattle-associated genotype C7 was detected containing 29 IS1111 copies. Overall, goats seem to shed more C. burnetii through vaginal mucus and in particular shed more and for longer via the rectal route than sheep. This is supported by the larger quantities of C. burnetii DNA detected in caprine nasal swabs and environmental samples compared to the ovine ones. Transmission of C. burnetii from cattle to small ruminants must also be considered.

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“…Even though the role of dogs in Q Fever epidemiology raises the interest of researchers in view of the One Health perspective, data about the spreading of this infection in canine populations are scant. In particular, investigations carried out in Italy were mainly performed on farm animals and wildlife [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ], but studies on companion animals are very limited [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Retrospective Study on the Occurrence of Antibodies against Coxiella burnetii in Dogs from Central Italy

Ebani

2020

Pathogens

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Coxiella burnetii, a cause of infection in humans and several animal species, is transmitted through inhalations and oral route but also tick bites. Its spreading in ruminants has been largely investigated, whereas data about the occurrence of this infection in canine population are scanty. In this retrospective study, blood serum samples of 516 dogs were tested by indirect immunofluorescence assay to detect antibodies against C. burnetii; 42 (8.13%) were positive with titers ranging from 1:64 to 1:512. The highest seroprevalences were detected in dogs aged > 5 years, employed in hunting activity and living in a peri-urban/rural environment. Diagnosis for C. burnetii infection should be always carried out in bitches with reproductive disorders. Moreover, in view of the zoonotic impact of this infection, asymptomatic dogs exposed to ticks’ bites and/or to contact with infected farm animals should be checked, too.

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Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

Cited by 13 publications

References 37 publications

Prevalence of Coxiella burnetii in bovine placentas in Hungary and Slovakia: Detection of a novel sequence type – Short communication

Prevalence of Coxiella burnetii in bovine placentas in Hungary and Slovakia: Detection of a novel sequence type – Short communication

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

Retrospective Study on the Occurrence of Antibodies against Coxiella burnetii in Dogs from Central Italy

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